SMURF1 Antibody (YA6771)

Cat. No.: HY-P87078: Data Sheet COA
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User Guide for Antibodies
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SMURF1 Antibody (YA6771) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SMURF1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

SMURF1 Antibody (YA6771) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SMURF1.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 86 kDa;

Observed band size: 87 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9HCE7

Gene ID

57154 [NCBI]

Immunogen

Recombinant protein within human SMURF1 aa

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Hela (lane 1(20μg)) 、MCF7 (lane 2(20μg)) 、HEK293 (lane 3(20μg)) and LNCap (lane 4(20μg)) using SMURF1 Antibody (HY-P87078) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

Function：E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Promotes ubiquitination and subsequent proteasomal degradation of MAVS (PubMed:23087404). Acts as an antagonist of TGF-beta signaling by ubiquitinating TGFBR1 and targeting it for degradation (PubMed:21791611). Plays a role in dendrite formation by melanocytes (PubMed:23999003)

Subcellular Localization：Cytoplasm,Cell membrane

Expression：
Tissue_Specificity: Expressed in melanocytes (PubMed:23999003)

Isoforms & Post-Translational Modification：Q9HCE7 has two isomers: Q9HCE7-1: 86114 Da (predicted); Q9HCE7-2: 83440 Da (predicted).
Auto-ubiquitinated in presence of NDFIP1 (PubMed:23087404)

Subunit：Interacts with TRAF4

RRID

AB_3719256

Synonyms

E3 ubiquitin-protein ligase SMURF1 antibody; hSMURF1 antibody; KIAA1625 antibody; Smad specific E3 ubiquitin ligase 1 antibody; SMAD specific E3 ubiquitin protein ligase 1 antibody; Smad ubiquitination regulatory factor 1 antibody; SMAD-specific E3 ubiquitin-protein ligase 1 antibody; SMUF1_HUMAN antibody; SMURF 1 antibody; smurf1 antibody;

Documentation

Select Batch:

Data Sheet (231 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)