Atg16L1 Antibody (YA2187)

Cat. No.: HY-P82442: Data Sheet COA
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Atg16L1 Antibody (YA2187) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Atg16L1.

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Atg16L1 Antibody (YA2187) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
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Atg16L1 Antibody (YA2187) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Atg16L1.

Host

Rabbit

Clonality

Recombinant,Monoclonal

분자량

Predicted band size: 68 kDa;

Observed band size: 68 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q676U5

Gene ID

55054 [NCBI]

Immunogen

Recombinant protein of human ATG16L1

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), A431 (lane 2, 15 μg), HCT116 (lane 3, 15 μg), HepG2 (lane 4, 15 μg), 3T3 (lane 5, 15 μg), and PC-12 (lane 6, 15 μg) using ATG16L1 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Plays an essential role in both canonical and non-canonical autophagy: interacts with ATG12-ATG5 to mediate the lipidation to ATG8 family proteins (MAP1LC3A, MAP1LC3B, MAP1LC3C, GABARAPL1, GABARAPL2 and GABARAP) (PubMed:23376921, PubMed:23392225, PubMed:24553140, PubMed:24954904, PubMed:27273576, PubMed:29317426, PubMed:30778222, PubMed:33909989). Acts as a molecular hub, coordinating autophagy pathways via distinct domains that support either canonical or non-canonical signaling (PubMed:29317426, PubMed:30778222). During canonical autophagy, interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to ATG8 proteins, to produce a membrane-bound activated form of ATG8 (PubMed:23376921, PubMed:23392225, PubMed:24553140, PubMed:24954904, PubMed:27273576). Thereby, controls the elongation of the nascent autophagosomal membrane (PubMed:23376921, PubMed:23392225, PubMed:24553140, PubMed:24954904, PubMed:27273576). As part of the ATG8 conjugation system with ATG5 and ATG12, required for recruitment of LRRK2 to stressed lysosomes and induction of LRRK2 kinase activity in response to lysosomal stress (By similarity). Also involved in non-canonical autophagy, a parallel pathway involving conjugation of ATG8 proteins to single membranes at endolysosomal compartments, probably by catalyzing conjugation of phosphatidylserine (PS) to ATG8 (PubMed:33909989). Non-canonical autophagy plays a key role in epithelial cells to limit lethal infection by influenza A (IAV) virus (By similarity). Regulates mitochondrial antiviral signaling (MAVS)-dependent type I interferon (IFN-I) production (PubMed:22749352, PubMed:25645662). Negatively regulates NOD1- and NOD2-driven inflammatory cytokine response (PubMed:24238340). Instead, promotes an autophagy-dependent antibacterial pathway together with NOD1 or NOD2 (PubMed:20637199). Plays a role in regulating morphology and function of Paneth cell (PubMed:18849966)

Subcellular Localization：Cytoplasm; Preautophagosomal structure membrane; Peripheral membrane protein; Endosome membrane; Peripheral membrane protein; Lysosome membrane; Peripheral membrane protein

Isoforms & Post-Translational Modification：Q676U5 has 5 isomers: Q676U5-1: 68265 Da (predicted); Q676U5-2: 66296 Da (predicted); Q676U5-3: 53220 Da (predicted); Q676U5-4: 47686 Da (predicted); Q676U5-5: 49486 Da (predicted).
Proteolytic cleavage by activated CASP3 leads to degradation and may regulate autophagy upon cellular stress and apoptotic stimuli;Phosphorylation at Ser-139 promotes association with the ATG12-ATG5 conjugate to form the ATG12-ATG5-ATG16L1 complex

Subunit：Homodimer (PubMed:25484072). Homooligomer (By similarity). Heterooligomer with ATG16L2 (By similarity). Interacts with WIPI1 (PubMed:28561066). Interacts with WIPI2 (PubMed:24954904, PubMed:28561066). Interacts with RB1CC1; the interaction is required for ULK1 complex-dependent autophagy (PubMed:23262492, PubMed:23392225, PubMed:24954904). Interacts with ATG5 (PubMed:23202584, PubMed:24191030, PubMed:25484072, PubMed:26812546). Part of the minor complex composed of 4 sets of ATG12-ATG5 and ATG16L1 (400 kDa); this complex interacts with ATG3 leading to disruption of ATG7 interaction and promotion of ATG8-like proteins lipidation (PubMed:23202584, PubMed:24191030, PubMed:26083323, PubMed:29317426, PubMed:30778222). Part of the major complex composed of 8 sets of ATG12-ATG5 and ATG16L1 (800 kDa) (PubMed:23202584, PubMed:26083323, PubMed:29317426, PubMed:30778222). Interacts with RAB33B (GTP- and GDP-bound forms); the complex consists of a tetramer where two RAB33B molecules bind independently one molecule of the ATG16L1 homodimer; the interaction promotes ATG12-ATG5-ATG16L1 complex recruitment to phagophores (PubMed:21808068, PubMed:32960676). Interacts (via WD repeats) with TMEM59; the interaction mediates unconventional autophagic activity of TMEM59 (PubMed:23376921, PubMed:27273576). Interacts with TLR2 (PubMed:23376921). Interacts (via WD repeats) with MEFV (PubMed:26347139). Interacts with PPP1CA; the interaction dephosphorylates ATG16L1 causing dissociation of ATG12-ATG5-ATG16L1 complex (PubMed:26083323). Interacts (via N-terminal) with CLTC (PubMed:20639872). Interacts with NOD1 (By similarity). Interacts with NOD2 (PubMed:20637199). Interacts with TUFM (PubMed:22749352). Interacts with TRIM16 (PubMed:30143514). Interacts (via WD repeats) with SPATA33 (PubMed:33087875). Interacts with IRGM (PubMed:25891078)

Database

Entrez Gene: 55054 Human ; 77040 Mouse ; 363278 Rat

SwissProt: Q676U5 Human ; Q8C0J2 Mouse ;

OMIM: 611081 Human

Research Field

Cell Biology

Synonyms

ATG16L1; APG16L; Autophagy-related protein 16-1; APG16-like 1

각종 서류

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Data Sheet (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Atg16L1 Antibody (YA2187) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Atg16L1 Antibody (YA2187)

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Atg16L1 Antibody (YA2187) Related Classifications

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