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Gli1 Antibody (YA414)

Customer Review

Based on 1 Customer Validation

Gli1 Antibody (YA414) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Gli1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for Gli1 Antibody (YA414)
  • Experimental Validation Results for Gli1 Antibody (YA414)
  • Experimental Validation Results for Gli1 Antibody (YA414)
  • Gli1 Antibody (YA414) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

Gli1 Antibody (YA414) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Gli1.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 150 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 118 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Gli1.AA range:38-82.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102317

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Gli1 Antibody (YA414)
    Western blot analysis of extracts from K-562(lane 2(20μg)，Hela(lane 3(20μg) and HEK293(lane 4(20μg) using Gli1(HY-P80143 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Gli1 Antibody (YA414)
    Immunohistochemical analysis of paraffin-embedded rat testis tissue using Gli1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Gli1 Antibody (YA414)
    Immunohistochemical analysis of paraffin-embedded rat testis tissue using Gli1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Gli1 acts as a transcriptional activator. Binds to the DNA consensus sequence 5'-GACCACCCA-3'. Regulates the transcription of specific genes during normal development. Plays a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling. Plays a role in cell proliferation and differentiation via its role in SHH signaling; Acts as a transcriptional activator, but activates a different set of genes than isoform 1. Activates expression of CD24, unlike isoform 1. Mediates SHH signaling. Promotes cancer cell migration[1][2][3][4][5][6][7][8][9][10].

  • Subcellular Localization

    Cytoplasm; Nucleus; Cytoplasm; Nucleus

  • Expression


    Tissue_specificity:Protein expression was detected in the testes (PubMed: 2105456) . It was also expressed in the testes, uterine myometrium, and fallopian tubes. Expression was also found in brain tissue, with the highest expression levels in the cerebellum, optic nerve, and olfactory tract (PubMed: 19878745) . Isomer 1 was detected in the brain, spleen, pancreas, liver, kidney, and placenta; isomer 2 was not detected in these tissues (PubMed: 19706761) .

    Induction:Isoform 1 and isoform 2 are amplified in glioblastoma cells

  • Subunit

    Interacts with STK36 (PubMed:10806483). Interacts with ZIC1; the interaction enhances transcription activation (PubMed:11238441)

  • SwissProt ID

    P08151

  • Gene ID
    14632 [NCBI]
  • Research Field

    Epigenetics and Nuclear Signaling

Gli1 Antibody (YA414) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K-562(lane 2(20μg)，Hela(lane 3(20μg) and HEK293(lane 4(20μg) using Gli1(HY-P80143 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat testis tissue using Gli1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat testis tissue using Gli1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Gli1 Antibody (YA414) Product Image
    1/16
  • Experimental Validation Results for Gli1 Antibody (YA414)
  • Experimental Validation Results for Gli1 Antibody (YA414)
  • Experimental Validation Results for Gli1 Antibody (YA414)
  • Gli1 Antibody (YA414) Product Image
  • Experimental Validation Results for Gli1 Antibody (YA414)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K-562(lane 2(20μg)，Hela(lane 3(20μg) and HEK293(lane 4(20μg) using Gli1(HY-P80143 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Gli1 Antibody (YA414)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat testis tissue using Gli1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Gli1 Antibody (YA414)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat testis tissue using Gli1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Gli1 Antibody (YA414)
  • Experimental Validation Results for Gli1 Antibody (YA414)
  • Experimental Validation Results for Gli1 Antibody (YA414)
  • Gli1 Antibody (YA414) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K-562(lane 2(20μg)，Hela(lane 3(20μg) and HEK293(lane 4(20μg) using Gli1(HY-P80143 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Gli1 Antibody (YA414) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat testis tissue using Gli1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Gli1 Antibody (YA414) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat testis tissue using Gli1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Gli1 Antibody (YA414) Product Image WB
  • Gli1 Antibody (YA414) Product Image IHC-P
  • Gli1 Antibody (YA414) Product Image IHC-P