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CD3 epsilon Antibody (YA6260)

(Synonyms: T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD antigen CD3e; )
Customer Review

Based on 1 Customer Validation

CD3 epsilon Antibody (YA6260) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD3 epsilon.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for CD3 epsilon Antibody (YA6260)
  • Experimental Validation Results for CD3 epsilon Antibody (YA6260)
  • Experimental Validation Results for CD3 epsilon Antibody (YA6260)
  • Experimental Validation Results for CD3 epsilon Antibody (YA6260)
  • Experimental Validation Results for CD3 epsilon Antibody (YA6260)
  • Experimental Validation Results for CD3 epsilon Antibody (YA6260)
  • Experimental Validation Results for CD3 epsilon Antibody (YA6260)
  • CD3 epsilon Antibody (YA6260) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:200-1:1000 1:1000-1:5000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

CD3 epsilon Antibody (YA6260) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD3 epsilon.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 23 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 23 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719184

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD3 epsilon Antibody (YA6260)
    Western blot analysis of extracts from Jurkat (lane2, 20μg), Mouse bone tissue (lane3, 20μg), Rat spleen tissue (lane4, 20μg) and Mouse spleen tissue (lane5, 20μg) using CD3 epsilon Antibody (HY-P86568). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD3 epsilon Antibody (YA6260)
    Immunohistochemical analysis of paraffin-embedded mouse appendix tissue using CD3 epsilon Antibody (HY-P86568, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD3 epsilon Antibody (YA6260)
    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using CD3 epsilon Antibody (HY-P86568, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD3 epsilon Antibody (YA6260)
    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue using CD3 epsilon Antibody (HY-P86568, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD3 epsilon Antibody (YA6260)
    Immunohistochemical analysis of paraffin-embedded rat spleen tissue using CD3 epsilon Antibody (HY-P86568, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD3 epsilon Antibody (YA6260)
    Immunohistochemical analysis of paraffin-embedded rat lymph node tissue using CD3 epsilon Antibody (HY-P86568, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD3 epsilon Antibody (YA6260)
    Immunohistochemical analysis of paraffin-embedded rat thymus tissue using CD3 epsilon Antibody (HY-P86568, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    CD3 epsilon is a Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Also participates in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region. In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx[1][2][3][4].

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein

  • Subunit

    The TCR-CD3 complex is composed of a CD3D/CD3E and a CD3G/CD3E heterodimers that preferentially associate with TCRalpha and TCRbeta, respectively, to form TCRalpha/CD3E/CD3G and TCRbeta/CD3G/CD3E trimers. In turn, the hexamer interacts with CD3Z homodimer to form the TCR-CD3 complex. Alternatively, TCRalpha and TCRbeta can be replaced by TCRgamma and TCRdelta. Interacts with CD6 (PubMed:15294938). Interacts (via Proline-rich sequence) with NCK1; the interaction is ligand dependent but independent of tyrosine kinase activation (PubMed:12110186, PubMed:15972658, PubMed:38614099). Interacts with NUMB; this interaction is important for TCR-CD3 internalization and subsequent degradation (PubMed:26507128)

  • SwissProt ID

    P07766

  • Gene ID
    916 [NCBI]
  • Synonyms

    T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD antigen CD3e;

[1]. Barber EK, et al. The CD4 and CD8 antigens are coupled to a protein-tyrosine kinase (p56lck) that phosphorylates the CD3 complex. Proc Natl Acad Sci U S A. 1989 May;86(9):3277-81. [Content Brief]

[2]. Borroto A, et al. The CD3 epsilon subunit of the TCR contains endocytosis signals. J Immunol. 1999 Jul 1;163(1):25-31. [Content Brief]

[3]. Martin-Blanco N, et al. CD3ε recruits Numb to promote TCR degradation. Int Immunol. 2016 Mar;28(3):127-37. [Content Brief]

[4]. Deng S, et al. ITPRIPL1 binds CD3ε to impede T cell activation and enable tumor immune evasion. Cell. 2024 Apr 25;187(9):2305-2323.e33. [Content Brief]

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CD3 epsilon Antibody (YA6260) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane2, 20μg), Mouse bone tissue (lane3, 20μg), Rat spleen tissue (lane4, 20μg) and Mouse spleen tissue (lane5, 20μg) using CD3 epsilon Antibody (HY-P86568). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse appendix tissue using CD3 epsilon Antibody (HY-P86568, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using CD3 epsilon Antibody (HY-P86568, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse thymus tissue using CD3 epsilon Antibody (HY-P86568, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat spleen tissue using CD3 epsilon Antibody (HY-P86568, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat lymph node tissue using CD3 epsilon Antibody (HY-P86568, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat thymus tissue using CD3 epsilon Antibody (HY-P86568, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CD3 epsilon Antibody (YA6260) Product Image
    1/16
  • Experimental Validation Results for CD3 epsilon Antibody (YA6260)
  • Experimental Validation Results for CD3 epsilon Antibody (YA6260)
  • Experimental Validation Results for CD3 epsilon Antibody (YA6260)
  • Experimental Validation Results for CD3 epsilon Antibody (YA6260)
  • Experimental Validation Results for CD3 epsilon Antibody (YA6260)
  • Experimental Validation Results for CD3 epsilon Antibody (YA6260)
  • Experimental Validation Results for CD3 epsilon Antibody (YA6260)
  • CD3 epsilon Antibody (YA6260) Product Image
  • Experimental Validation Results for CD3 epsilon Antibody (YA6260)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane2, 20μg), Mouse bone tissue (lane3, 20μg), Rat spleen tissue (lane4, 20μg) and Mouse spleen tissue (lane5, 20μg) using CD3 epsilon Antibody (HY-P86568). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD3 epsilon Antibody (YA6260)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse appendix tissue using CD3 epsilon Antibody (HY-P86568, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD3 epsilon Antibody (YA6260)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using CD3 epsilon Antibody (HY-P86568, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD3 epsilon Antibody (YA6260)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse thymus tissue using CD3 epsilon Antibody (HY-P86568, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD3 epsilon Antibody (YA6260)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat spleen tissue using CD3 epsilon Antibody (HY-P86568, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD3 epsilon Antibody (YA6260)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat lymph node tissue using CD3 epsilon Antibody (HY-P86568, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD3 epsilon Antibody (YA6260)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat thymus tissue using CD3 epsilon Antibody (HY-P86568, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD3 epsilon Antibody (YA6260)
  • Experimental Validation Results for CD3 epsilon Antibody (YA6260)
  • Experimental Validation Results for CD3 epsilon Antibody (YA6260)
  • Experimental Validation Results for CD3 epsilon Antibody (YA6260)
  • Experimental Validation Results for CD3 epsilon Antibody (YA6260)
  • Experimental Validation Results for CD3 epsilon Antibody (YA6260)
  • Experimental Validation Results for CD3 epsilon Antibody (YA6260)
  • CD3 epsilon Antibody (YA6260) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Jurkat (lane2, 20μg), Mouse bone tissue (lane3, 20μg), Rat spleen tissue (lane4, 20μg) and Mouse spleen tissue (lane5, 20μg) using CD3 epsilon Antibody (HY-P86568). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • CD3 epsilon Antibody (YA6260) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse appendix tissue using CD3 epsilon Antibody (HY-P86568, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CD3 epsilon Antibody (YA6260) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using CD3 epsilon Antibody (HY-P86568, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CD3 epsilon Antibody (YA6260) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue using CD3 epsilon Antibody (HY-P86568, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CD3 epsilon Antibody (YA6260) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue using CD3 epsilon Antibody (HY-P86568, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CD3 epsilon Antibody (YA6260) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat lymph node tissue using CD3 epsilon Antibody (HY-P86568, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CD3 epsilon Antibody (YA6260) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat thymus tissue using CD3 epsilon Antibody (HY-P86568, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CD3 epsilon Antibody (YA6260) Product Image WB
  • CD3 epsilon Antibody (YA6260) Product Image IHC-P
  • CD3 epsilon Antibody (YA6260) Product Image IHC-P
  • CD3 epsilon Antibody (YA6260) Product Image IHC-P
  • CD3 epsilon Antibody (YA6260) Product Image IHC-P
  • CD3 epsilon Antibody (YA6260) Product Image IHC-P
  • CD3 epsilon Antibody (YA6260) Product Image IHC-P