RBBP4 Antibody (YA1843)(PBS only)

Cat. No.: HY-P82098A: Data Sheet User Guide for Antibodies
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RBBP4 Antibody (YA1843) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RBBP4.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

RBBP4 Antibody (YA1843) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RBBP4.

Host

Rabbit

Molecular Weight

Predicted band size: 48 kDa;

Observed band size: 48 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat, Hamster

SwissProt ID

Q09028

Gene ID

5928 [NCBI]

Immunogen

A synthetic peptide of human RbAp48

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IP IP: Immunoprecipitation	1:20

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA (PubMed:10866654). Component of the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair (PubMed:8858152). Component of the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression (PubMed:9150135). Component of the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling (PubMed:16428440, PubMed:28977666, PubMed:39460621). Component of the PRC2 complex, which promotes repression of homeotic genes during development (PubMed:29499137, PubMed:31959557). Component of the NURF (nucleosome remodeling factor) complex (PubMed:14609955, PubMed:15310751)

Subcellular Localization：Nucleus; Chromosome, telomere

Expression：
Tissue_specificity:Expression in neuroblastoma cells

Isoforms & Post-Translational Modification：Q09028 has 4 isomers: Q09028-1: 47656 Da (predicted); Q09028-2: 47585 Da (predicted); Q09028-3: 46158 Da (predicted); Q09028-4: 43482 Da (predicted).

Subunit：Binds directly to helix 1 of the histone fold of histone H4, a region that is not accessible when H4 is in chromatin (PubMed:24920672, PubMed:8858152, PubMed:9427644). Subunit of the chromatin assembly factor 1 (CAF-1) complex, which is composed of RBBP4, CHAF1B and CHAF1A (PubMed:8858152). Subunit of the core histone deacetylase (HDAC) complex, which is composed of HDAC1, HDAC2, RBBP4 and RBBP7 (PubMed:9150135). The core HDAC complex associates with SIN3A, ARID4B/SAP180, SAP18, SAP30, SAP130, SUDS3/SAP45 and possibly ARID4A/RBP1 and ING1 to form the SIN3 HDAC complex (PubMed:11118440, PubMed:11784859, PubMed:9150135, PubMed:9651585). Component of the nucleosome remodeling and deacetylase (NuRD) repressor complex, composed of core proteins MTA1, MTA2, MTA3, RBBP4, RBBP7, HDAC1, HDAC2, MBD2, MBD3, and peripherally associated proteins CDK2AP1, CDK2AP2, GATAD2A, GATAD2B, CHD3, CHD4 and CHD5 (PubMed:10444591, PubMed:11102443, PubMed:16428440, PubMed:28977666, PubMed:33283408, PubMed:9790534, PubMed:39460621). The exact stoichiometry of the NuRD complex is unknown, and some subunits such as MBD2 and MBD3, GATAD2A and GATAD2B, and CHD3, CHD4 and CHD5 define mutually exclusive NuRD complexes (PubMed:16428440, PubMed:28977666, PubMed:33283408). Interacts with ZNF512B; the interaction is direct and may play a role in repressing gene expression (PubMed:39460621). The NuRD complex may also interact with MBD3L1 and MBD3L2 (PubMed:15456747, PubMed:15701600). Component of the PRC2 complex, which consists of the core subunits EED, EZH1 or EZH2, SUZ12, and RBBP4, and various combinations of accessory subunits including AEBP2, JARID2, PHF19, MTF2 and EPOP (PubMed:12351676, PubMed:12435631, PubMed:29499137, PubMed:31959557). Forms a monomeric PRC2.2 (class 2) complex consisting of at least SUZ12, RBBP4, AEBP2 and JARID2 (PubMed:29499137). Forms a dimeric PRC2.1 (class 1, PRC-PCL) complex consisting of at least SUZ12, RBBP4, and PHF19; PHF19 stabilizes the dimeric structure which enhances PRC2 interaction with chromatin (PubMed:31959557). Component of the NURF-1 ISWI chromatin remodeling complex (also called the nucleosome-remodeling factor (NURF) complex) at least composed of SMARCA1 (isoform 2), BPTF, RBBP4 and RBBP7 (PubMed:14609955, PubMed:15310751). Within the complex interacts with isoform 2 of SMARCA1 (PubMed:14609955, PubMed:15310751). Component of the BPFT-SMARCA1 complex at least composed of SMARCA1 (isoform 1), BPFT, RBBP4 and RBBP7; the complex is catalytically inactive and does not remodel chromatin (PubMed:15310751). Within the complex interacts with isoform 1 of SMARCA1 (PubMed:15310751). Interacts with the ISWI chromatin remodeling complex component SMARCA5; the interaction is direct (PubMed:12198550). Interacts with the viral protein-binding domain of the retinoblastoma protein (RB1) (PubMed:10734134, PubMed:7503932). Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2 (PubMed:17531812, PubMed:17671431). The complex exists in quiescent cells where it represses cell cycle-dependent genes (PubMed:17531812, PubMed:17671431). It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2 (PubMed:17531812, PubMed:17671431). Found in a complex composed of at least SINHCAF, SIN3A, HDAC1, SAP30, RBBP4, OGT and TET1 (By similarity). Interacts with ZNF827; the interaction is direct and recruits RBBP4 to telomeres (PubMed:30045876). Interacts with MTA1; the interaction is direct and mutually exclusive with binding histone H4 (PubMed:12920132, PubMed:24920672). Interacts with ARMC12 (via ARM domains) (PubMed:30026490). Interacts with BRCA1 (PubMed:10220405). Interacts with CDK2AP1 (PubMed:20523938). Interacts with CREBBP, and this interaction may be enhanced by the binding of phosphorylated CREB1 to CREBBP (PubMed:10866654). Interacts with ERCC6 (PubMed:26030138). Interacts with HDAC7 (By similarity). Interacts with PHF6 (PubMed:24554700). Interacts with PWWP2B (By similarity). Interacts with SPEN/MINT (PubMed:11331609). Interacts with SUV39H1 (By similarity)

Research Field

Epigenetics and Nuclear Signaling

Synonyms

NURF55; RBAP48; lin-53

Documentation

User Guide for Antibodies (1077 KB)