Osteopontin Antibody (YA6362)(PBS only)

Cat. No.: HY-P86670A: Ficha de datos User Guide for Antibodies
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Osteopontin Antibody (YA6362) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Osteopontin.

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Tamaño		Stock
50 μL		Obtener un presupuesto
100 μL		Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Descripciòn

Descripciòn

Osteopontin Antibody (YA6362) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Osteopontin.

Host

Rabbit

Peso molecular

Predicted band size: 35 kDa

Species Reactivity

Human

SwissProt ID

P10451

Gene ID

6696 [NCBI]

Immunogen

Recombinant human Osteopontin protein aa 17-314/314

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-500
WB WB: Western Blot	1:500-2000
IHC-F IHC-F: Immunohistochemistry-Frozen	1:100-500
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-500

Pureza	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Background

Function：Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction; Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity

Subcellular Localization：Secreted

Expression：
Tissue_specificity:Protein levels were detected in cerebrospinal fluid and urine (PubMed:25326458, PubMed:36213313, PubMed:37453717) . In bone. Found in plasma.

Isoforms & Post-Translational Modification：P10451 has 4 isomers: P10451-1: 35423 Da (predicted); P10451-3: 32355 Da (predicted); P10451-4: 33017 Da (predicted); P10451-5: 33843 Da (predicted).
Forms covalent cross-links mediated by transglutaminase TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers, increasing its collagen binding properties;Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif (PubMed:15869464, PubMed:22582013, PubMed:26091039). The phosphorylated form inhibits hydroxyapatite crystallization. Dephosphorylation via a mechanism involving ALPL/TNAP promotes hydroxyapatite crystallization (By similarity);O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62

Subunit：Interacts (via N-terminus) with integrin ITGA9:ITGB1

Synonyms

BNSP; Bone sialoprotein 1; BSP I; BSPI; Early T lymphocyte activation 1; ETA 1; ETA1; MGC110940; Nephropontin; OPN; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; OSTP_HUMAN; secreted phosphoprotein 1(osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); Secreted phosphoprotein 1; SPP 1; SPP-1; SPP1; SPP1/CALPHA1 fusion; Urinary stone protein; Uropontin.

Documentación

User Guide for Antibodies (1077 KB)