Osteopontin Antibody (YA6362)

Cat. No.: HY-P86670: Data Sheet COA
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Osteopontin Antibody (YA6362) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Osteopontin.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Osteopontin Antibody (YA6362) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Osteopontin.

Host

Rabbit

Clonality

Monoclonal

Molecular Weight

Predicted band size: 35 kDa;

Observed band size: 63 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P10451

Gene ID

6696 [NCBI]

Immunogen

Recombinant human Osteopontin protein aa 17-314/314

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-500
WB WB: Western Blot	1:500-2000
IHC-F IHC-F: Immunohistochemistry-Frozen	1:100-500
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-500

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Solution

Formulation

Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

ALL WB IHC-P IF

Western blot analysis of extracts from Recombinant human Osteopontin protein(50ng)using Osteopontin Antibody (HY-P86670). Proteins were transferred to a NC membrane and blocked with 5% Non-fat milk powder in TBST for 1 hour at room temperature. The primary antibody (1/1000) was used in 5% Non-fat milk powder in TBST at 4°C overnight. HRP-conjugated Goat Anti-Rabbit Secondary Antibody (1/20,000) was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using Osteopontin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 15 minutes. The tissues were blocked in 10% normal goat serum for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (Osteopontin,HY-P86670, 1/200) at 4°C overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue using Osteopontin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 15 minutes. The tissues were blocked in 10% normal goat serum for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (Osteopontin,HY-P86670, 1/200) at 4°C overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunofluorescence analysis of paraffin-embedded human lung cancer using Osteopontin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 15 minutes. The tissues were blocked in 10% normal goat serum for 30 minutes at room temperature, then incubated with Osteopontin antibody (HY-P86670) at 1/200 dilution overnight at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003) was used as the secondary antibody at 1/200 dilution. The Nuclear counterstain was DAPI (Blue).
Immunofluorescence analysis of paraffin-embedded human kidney using Osteopontin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 15 minutes. The tissues were blocked in 10% normal goat serum for 30 minutes at room temperature, then incubated with Osteopontin antibody (HY-P86670) at 1/200 dilution overnight at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003) was used as the secondary antibody at 1/200 dilution. The Nuclear counterstain was DAPI (Blue).
Immunofluorescence analysis of paraffin-embedded mouse liver using Osteopontin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 15 minutes. The tissues were blocked in 10% normal goat serum for 30 minutes at room temperature, then incubated with Osteopontin antibody (HY-P86670) at 1/200 dilution overnight at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003) was used as the secondary antibody at 1/200 dilution. The Nuclear counterstain was DAPI (Blue).

Background

Function：Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction; Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity

Subcellular Localization：Secreted

Expression：
Tissue_specificity:Protein levels were detected in cerebrospinal fluid and urine (PubMed:25326458, PubMed:36213313, PubMed:37453717) . In bone. Found in plasma.

Isoforms & Post-Translational Modification：P10451 has 4 isomers: P10451-1: 35423 Da (predicted); P10451-3: 32355 Da (predicted); P10451-4: 33017 Da (predicted); P10451-5: 33843 Da (predicted).
Forms covalent cross-links mediated by transglutaminase TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers, increasing its collagen binding properties;Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif (PubMed:15869464, PubMed:22582013, PubMed:26091039). The phosphorylated form inhibits hydroxyapatite crystallization. Dephosphorylation via a mechanism involving ALPL/TNAP promotes hydroxyapatite crystallization (By similarity);O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62

Subunit：Interacts (via N-terminus) with integrin ITGA9:ITGB1

Synonyms

BNSP; Bone sialoprotein 1; BSP I; BSPI; Early T lymphocyte activation 1; ETA 1; ETA1; MGC110940; Nephropontin; OPN; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; OSTP_HUMAN; secreted phosphoprotein 1(osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); Secreted phosphoprotein 1; SPP 1; SPP-1; SPP1; SPP1/CALPHA1 fusion; Urinary stone protein; Uropontin.

Documentation

Select Batch:

Data Sheet (234 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)