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Osteopontin Antibody (YA6362)

(Synonyms: BNSP; Bone sialoprotein 1; BSP I; BSPI; Early T lymphocyte activation 1; ETA 1; ETA1; MGC110940; Nephropontin; OPN; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; OSTP_HUMAN; secreted phosphoprotein 1(osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); Secreted phosphoprotein 1; SPP 1; SPP-1; SPP1; SPP1/CALPHA1 fusion; Urinary stone protein; Uropontin.)
Customer Review

Based on 1 Customer Validation

Osteopontin Antibody (YA6362) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Osteopontin.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    IHC-P, WB, IHC-F, IF-Tissue

  • Reactivity :

    Human

  • Formulation:

    Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P IF
  • Experimental Validation Results for Osteopontin Antibody (YA6362)
  • Experimental Validation Results for Osteopontin Antibody (YA6362)
  • Experimental Validation Results for Osteopontin Antibody (YA6362)
  • Experimental Validation Results for Osteopontin Antibody (YA6362)
  • Experimental Validation Results for Osteopontin Antibody (YA6362)
  • Experimental Validation Results for Osteopontin Antibody (YA6362)
  • Osteopontin Antibody (YA6362) Product Image
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Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:100-500 1:500-2000 1:100-500 1:100-500

Product Details

Description

Osteopontin Antibody (YA6362) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Osteopontin.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 63 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 35 kDa
Immunogen

Recombinant human Osteopontin protein aa 17-314/314

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Osteopontin Antibody (YA6362)
    Western blot analysis of extracts from Recombinant human Osteopontin protein(50ng)using Osteopontin Antibody (HY-P86670). Proteins were transferred to a NC membrane and blocked with 5% Non-fat milk powder in TBST for 1 hour at room temperature. The primary antibody (1/1000) was used in 5% Non-fat milk powder in TBST at 4°C overnight. HRP-conjugated Goat Anti-Rabbit Secondary Antibody (1/20,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Osteopontin Antibody (YA6362)
    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using Osteopontin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 15 minutes. The tissues were blocked in 10% normal goat serum for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (Osteopontin,HY-P86670, 1/200) at 4°C overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Osteopontin Antibody (YA6362)
    Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue using Osteopontin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 15 minutes. The tissues were blocked in 10% normal goat serum for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (Osteopontin,HY-P86670, 1/200) at 4°C overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Osteopontin Antibody (YA6362)
    Immunofluorescence analysis of paraffin-embedded human lung cancer using Osteopontin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 15 minutes. The tissues were blocked in 10% normal goat serum for 30 minutes at room temperature, then incubated with Osteopontin antibody (HY-P86670) at 1/200 dilution overnight at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003) was used as the secondary antibody at 1/200 dilution. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Osteopontin Antibody (YA6362)
    Immunofluorescence analysis of paraffin-embedded human kidney using Osteopontin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 15 minutes. The tissues were blocked in 10% normal goat serum for 30 minutes at room temperature, then incubated with Osteopontin antibody (HY-P86670) at 1/200 dilution overnight at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003) was used as the secondary antibody at 1/200 dilution. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Osteopontin Antibody (YA6362)
    Immunofluorescence analysis of paraffin-embedded mouse liver using Osteopontin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 15 minutes. The tissues were blocked in 10% normal goat serum for 30 minutes at room temperature, then incubated with Osteopontin antibody (HY-P86670) at 1/200 dilution overnight at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003) was used as the secondary antibody at 1/200 dilution. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Osteopontin (OPN) is a secreted, multifunctional, extracellular matrix (ECM), calcium-binding, glycosylated phosphoprotein that is associated with various normal biological functions and pathological processes. OPN is physiologically produced by a wide range of cells and has been identified in various body fluids, playing a crucial role in bone remodeling and vascularization. OPN is also expressed by multiple immune cells, acting as a chemotactic factor, a mediator of cell activation and cytokine production, and an anti-apoptotic factor, thereby regulating inflammation and immune responses. OPN can be involved in the tumorigenesis of various cancer types, including head and neck squamous cell carcinoma (HNSCC), by promoting cell proliferation, adhesion, invasion, and migration through multiple signaling pathways[1][2].

  • Subcellular Localization

    Secreted

  • Expression


    Tissue_specificity:Protein levels were detected in cerebrospinal fluid and urine (PubMed:25326458, PubMed:36213313, PubMed:37453717) . In bone. Found in plasma.

  • Isoforms & Post-Translational Modification

    P10451 has 4 isomers: P10451-1: 35423 Da (predicted); P10451-3: 32355 Da (predicted); P10451-4: 33017 Da (predicted); P10451-5: 33843 Da (predicted).
    Forms covalent cross-links mediated by transglutaminase TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers, increasing its collagen binding properties;Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif (PubMed:15869464, PubMed:22582013, PubMed:26091039). The phosphorylated form inhibits hydroxyapatite crystallization. Dephosphorylation via a mechanism involving ALPL/TNAP promotes hydroxyapatite crystallization (By similarity);O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62

  • Subunit

    Interacts (via N-terminus) with integrin ITGA9:ITGB1

  • SwissProt ID

    P10451

  • Gene ID
    6696 [NCBI]
  • Synonyms

    BNSP; Bone sialoprotein 1; BSP I; BSPI; Early T lymphocyte activation 1; ETA 1; ETA1; MGC110940; Nephropontin; OPN; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; OSTP_HUMAN; secreted phosphoprotein 1(osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); Secreted phosphoprotein 1; SPP 1; SPP-1; SPP1; SPP1/CALPHA1 fusion; Urinary stone protein; Uropontin.

References

[1]. El-Zawawy H T, et al. Osteopontin as a marker in thyroid disease: relation to body mass index[J]. Endocrine and Metabolic Science, 2020, 1(1-2): 100049.

[2]. Ghita I, et al. Cytokine profiling in plasma distinguishes the histological inflammatory subtype of head and neck squamous cell carcinoma and a novel regulatory role of osteopontin[J]. Frontiers in oral health, 2022, 3: 993638. [Content Brief]

Osteopontin Antibody (YA6362) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Recombinant human Osteopontin protein(50ng)using Osteopontin Antibody (HY-P86670). Proteins were transferred to a NC membrane and blocked with 5% Non-fat milk powder in TBST for 1 hour at room temperature. The primary antibody (1/1000) was used in 5% Non-fat milk powder in TBST at 4°C overnight. HRP-conjugated Goat Anti-Rabbit Secondary Antibody (1/20,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using Osteopontin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 15 minutes. The tissues were blocked in 10% normal goat serum for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (Osteopontin,HY-P86670, 1/200) at 4°C overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue using Osteopontin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 15 minutes. The tissues were blocked in 10% normal goat serum for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (Osteopontin,HY-P86670, 1/200) at 4°C overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunofluorescence analysis of paraffin-embedded human lung cancer using Osteopontin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 15 minutes. The tissues were blocked in 10% normal goat serum for 30 minutes at room temperature, then incubated with Osteopontin antibody (HY-P86670) at 1/200 dilution overnight at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003) was used as the secondary antibody at 1/200 dilution. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunofluorescence analysis of paraffin-embedded human kidney using Osteopontin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 15 minutes. The tissues were blocked in 10% normal goat serum for 30 minutes at room temperature, then incubated with Osteopontin antibody (HY-P86670) at 1/200 dilution overnight at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003) was used as the secondary antibody at 1/200 dilution. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunofluorescence analysis of paraffin-embedded mouse liver using Osteopontin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 15 minutes. The tissues were blocked in 10% normal goat serum for 30 minutes at room temperature, then incubated with Osteopontin antibody (HY-P86670) at 1/200 dilution overnight at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003) was used as the secondary antibody at 1/200 dilution. The Nuclear counterstain was DAPI (Blue).

  • Osteopontin Antibody (YA6362) Product Image
    1/16
  • Experimental Validation Results for Osteopontin Antibody (YA6362)
  • Experimental Validation Results for Osteopontin Antibody (YA6362)
  • Experimental Validation Results for Osteopontin Antibody (YA6362)
  • Experimental Validation Results for Osteopontin Antibody (YA6362)
  • Experimental Validation Results for Osteopontin Antibody (YA6362)
  • Experimental Validation Results for Osteopontin Antibody (YA6362)
  • Osteopontin Antibody (YA6362) Product Image
  • Experimental Validation Results for Osteopontin Antibody (YA6362)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Recombinant human Osteopontin protein(50ng)using Osteopontin Antibody (HY-P86670). Proteins were transferred to a NC membrane and blocked with 5% Non-fat milk powder in TBST for 1 hour at room temperature. The primary antibody (1/1000) was used in 5% Non-fat milk powder in TBST at 4°C overnight. HRP-conjugated Goat Anti-Rabbit Secondary Antibody (1/20,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Osteopontin Antibody (YA6362)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using Osteopontin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 15 minutes. The tissues were blocked in 10% normal goat serum for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (Osteopontin,HY-P86670, 1/200) at 4°C overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Osteopontin Antibody (YA6362)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue using Osteopontin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 15 minutes. The tissues were blocked in 10% normal goat serum for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (Osteopontin,HY-P86670, 1/200) at 4°C overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Osteopontin Antibody (YA6362)
    1/1
    MedchemExpress Validation
     Immunofluorescence analysis of paraffin-embedded human lung cancer using Osteopontin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 15 minutes. The tissues were blocked in 10% normal goat serum for 30 minutes at room temperature, then incubated with Osteopontin antibody (HY-P86670) at 1/200 dilution overnight at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003) was used as the secondary antibody at 1/200 dilution. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Osteopontin Antibody (YA6362)
    1/1
    MedchemExpress Validation
     Immunofluorescence analysis of paraffin-embedded human kidney using Osteopontin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 15 minutes. The tissues were blocked in 10% normal goat serum for 30 minutes at room temperature, then incubated with Osteopontin antibody (HY-P86670) at 1/200 dilution overnight at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003) was used as the secondary antibody at 1/200 dilution. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Osteopontin Antibody (YA6362)
    1/1
    MedchemExpress Validation
     Immunofluorescence analysis of paraffin-embedded mouse liver using Osteopontin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 15 minutes. The tissues were blocked in 10% normal goat serum for 30 minutes at room temperature, then incubated with Osteopontin antibody (HY-P86670) at 1/200 dilution overnight at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003) was used as the secondary antibody at 1/200 dilution. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Osteopontin Antibody (YA6362)
  • Experimental Validation Results for Osteopontin Antibody (YA6362)
  • Experimental Validation Results for Osteopontin Antibody (YA6362)
  • Experimental Validation Results for Osteopontin Antibody (YA6362)
  • Experimental Validation Results for Osteopontin Antibody (YA6362)
  • Experimental Validation Results for Osteopontin Antibody (YA6362)
  • Osteopontin Antibody (YA6362) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Recombinant human Osteopontin protein(50ng)using Osteopontin Antibody (HY-P86670). Proteins were transferred to a NC membrane and blocked with 5% Non-fat milk powder in TBST for 1 hour at room temperature. The primary antibody (1/1000) was used in 5% Non-fat milk powder in TBST at 4°C overnight. HRP-conjugated Goat Anti-Rabbit Secondary Antibody (1/20,000) was used for 1 hour at room temperature.

  • Osteopontin Antibody (YA6362) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using Osteopontin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 15 minutes. The tissues were blocked in 10% normal goat serum for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (Osteopontin,HY-P86670, 1/200) at 4°C overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Osteopontin Antibody (YA6362) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue using Osteopontin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 15 minutes. The tissues were blocked in 10% normal goat serum for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (Osteopontin,HY-P86670, 1/200) at 4°C overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Osteopontin Antibody (YA6362) Product Image
    MedchemExpress Validation

    Immunofluorescence analysis of paraffin-embedded human lung cancer using Osteopontin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 15 minutes. The tissues were blocked in 10% normal goat serum for 30 minutes at room temperature, then incubated with Osteopontin antibody (HY-P86670) at 1/200 dilution overnight at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003) was used as the secondary antibody at 1/200 dilution. The Nuclear counterstain was DAPI (Blue).

  • Osteopontin Antibody (YA6362) Product Image
    MedchemExpress Validation

    Immunofluorescence analysis of paraffin-embedded human kidney using Osteopontin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 15 minutes. The tissues were blocked in 10% normal goat serum for 30 minutes at room temperature, then incubated with Osteopontin antibody (HY-P86670) at 1/200 dilution overnight at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003) was used as the secondary antibody at 1/200 dilution. The Nuclear counterstain was DAPI (Blue).

  • Osteopontin Antibody (YA6362) Product Image
    MedchemExpress Validation

    Immunofluorescence analysis of paraffin-embedded mouse liver using Osteopontin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 15 minutes. The tissues were blocked in 10% normal goat serum for 30 minutes at room temperature, then incubated with Osteopontin antibody (HY-P86670) at 1/200 dilution overnight at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003) was used as the secondary antibody at 1/200 dilution. The Nuclear counterstain was DAPI (Blue).

  • Osteopontin Antibody (YA6362) Product Image WB
  • Osteopontin Antibody (YA6362) Product Image IHC-P
  • Osteopontin Antibody (YA6362) Product Image IHC-P
  • Osteopontin Antibody (YA6362) Product Image IF
  • Osteopontin Antibody (YA6362) Product Image IF
  • Osteopontin Antibody (YA6362) Product Image IF