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Firefly Luciferase Antibody (YA1495)

(Synonyms: Luciferase; Firefly; Luciferin 4 monooxygenase)
Customer Review

Based on 1 Customer Validation

Firefly Luciferase Antibody (YA1495) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Firefly Luciferase.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Firefly

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Firefly Luciferase Antibody (YA1495)
  • Firefly Luciferase Antibody (YA1495) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:1000-1:2000 1:50-1:200

Product Details

Description

Firefly Luciferase Antibody (YA1495) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Firefly Luciferase.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Firefly
  • Observed Molecular Weight
    Observed band size: 55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 61 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from Firefly Luciferase aa152-201.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103856

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Firefly Luciferase Antibody (YA1495)
    Western blot analysis of extracts from HEK293T(lane 2(20ug) and HEK293T(lane 3(40ug) using Firefly Luciferase Antibody (HY-P81750) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Firefly Luciferase is a Produces green light with a wavelength of 562 nm

  • Subcellular Localization

    Peroxisome

  • SwissProt ID

    P08659

  • Gene ID
    116160065 [NCBI]
  • Synonyms

    Luciferase; Firefly; Luciferin 4 monooxygenase

  • Research Field

    Tags & Cell Markers

Firefly Luciferase Antibody (YA1495) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from HEK293T(lane 2(20ug) and HEK293T(lane 3(40ug) using Firefly Luciferase Antibody (HY-P81750) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Firefly Luciferase Antibody (YA1495) Product Image
    1/16
  • Experimental Validation Results for Firefly Luciferase Antibody (YA1495)
  • Firefly Luciferase Antibody (YA1495) Product Image
  • Experimental Validation Results for Firefly Luciferase Antibody (YA1495)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from HEK293T(lane 2(20ug) and HEK293T(lane 3(40ug) using Firefly Luciferase Antibody (HY-P81750) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Firefly Luciferase Antibody (YA1495)
  • Firefly Luciferase Antibody (YA1495) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from HEK293T(lane 2(20ug) and HEK293T(lane 3(40ug) using Firefly Luciferase Antibody (HY-P81750) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Firefly Luciferase Antibody (YA1495) Product Image WB