2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. Firefly Luciferase Antibody (YA1495)

Firefly Luciferase Antibody (YA1495)

Art. -Nr.: HY-P81750
COA
SDS
User Guide for Antibodies Technical Support

Firefly Luciferase Antibody (YA1495) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Firefly Luciferase.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Verification Image

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  • Beschreibung

Beschreibung

Firefly Luciferase Antibody (YA1495) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Firefly Luciferase.
Host

Rabbit
Clonality

Recombinant,Monoclonal
Molekulargewicht
Predicted band size: 61 kDa;
Observed band size: 55 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Firefly
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from Firefly Luciferase aa152-201.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:1000-1:2000
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:50-1:200
Sensitivity Endogenous Reinheit Affinity Chromatography
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Versand

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis of extracts from HEK293T(lane 2(20ug) and HEK293T(lane 3(40ug) using Firefly Luciferase Antibody (HY-P81750) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background
Function：Produces green light with a wavelength of 562 nm
Subcellular Localization：Peroxisome
RRID
Database

Entrez Gene: 116160065 

SwissProt: P08659 Firefly

Research Field

Tags & Cell Markers
Synonyms
Luciferase; Firefly; Luciferin 4 monooxygenase
Dokumentation
SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Firefly Luciferase Antibody (YA1495) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Firefly Luciferase Antibody (YA1495)
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HY-P81750
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SAFETY DATA SHEET (SDS)

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