EIF4E2 Antibody (YA8334)(PBS only)

Art. -Nr.: HY-P88650A: Data Sheet User Guide for Antibodies
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EIF4E2 Antibody (YA8334) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to EIF4E2.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Größe		Verfügbarkeit
50 μL		Angebot einholen
100 μL		Angebot einholen

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Beschreibung

Beschreibung

EIF4E2 Antibody (YA8334) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to EIF4E2.

Host

Mouse

Molekulargewicht

Predicted band size: 28.2 kDa

Species Reactivity

Human, Mouse

SwissProt ID

O60573

Immunogen

Full length human recombinant protein of human EIF4E2 produced in HEK293T cell.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-250
FC FC: Flow Cytometry	1:100

Reinheit	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Background

Function：Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation. Acts as a repressor of translation initiation (PubMed:17368478, PubMed:22751931, PubMed:25624349, PubMed:33581076, PubMed:9582349). In contrast to EIF4E, it is unable to bind eIF4G (EIF4G1, EIF4G2 or EIF4G3), suggesting that it acts by competing with EIF4E and block assembly of eIF4F at the cap (By similarity). In P-bodies, component of a complex that promotes miRNA-mediated translational repression (PubMed:28487484). Involved in virus-induced host response by mediating miRNA MIR34A-induced translational silencing which controls IFNB1 production by a negative feedback mechanism (PubMed:28487484, PubMed:33581076)|Component of the 4EHP-GYF2 complex, a multiprotein complex that acts as a repressor of translation initiation (PubMed:22751931, PubMed:35878012). In association with GIGYF2, assists ribosome-associated quality control (RQC) by sequestering the mRNA cap, blocking ribosome initiation and decreasing the translational load on problematic messages. Part of a pathway that works in parallel to RQC-mediated degradation of the stalled nascent polypeptide. GIGYF2 and EIF4E2 work downstream and independently of ZNF598, which seems to work as a scaffold that can recruit them to faulty mRNA even if alternative recruitment mechanisms may exist (PubMed:32726578)|(Microbial infection) Upon SARS coronavirus-2/SARS-CoV-2 infection, the interaction with non-structural protein 2 (nsp2) with GIGYF2 enhances GIGYF2 binding to EIF4E2 and increases repression of translation initiation of genes involved in antiviral innate immune response such as IFNB1

Subcellular Localization：Cytoplasm,Cytoplasm, P-body

Isoforms & Post-Translational Modification：O60573 has two isomers: O60573-1: 28362 Da (predicted); O60573-2: 27026 Da (predicted).
Ubiquitinated by ARIH1 (PubMed:14623119, PubMed:25624349)丨ISGylation enhances its cap structure-binding activity and translation-inhibition activity

Subunit：Interacts with EIF4EBP1, EIF4EBP2 and EIF4EBP3 (PubMed:15153109, PubMed:17368478)

Synonyms

4E-LP; 4EHP; EIF4EL3; IF4e

Dokumentation

User Guide for Antibodies (1077 KB)