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Cystatin C Antibody (YA480)

(Synonyms: CST3; Cystatin-C; Cystatin-3; Gamma-trace; Neuroendocrine basic polypeptide; Post-gamma-globulin)
Customer Review

Based on 1 Customer Validation

Cystatin C Antibody (YA480) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cystatin C.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
ALL WB IHC-P mIHC
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Cystatin C Antibody (YA480) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

Cystatin C Antibody (YA480) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cystatin C.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 13 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 16 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Cystatin C.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Cystatin C Antibody (YA480)
    Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), U-87MG (lane 2, 15 μg), BxPC-3 (lane 3, 15 μg), Mouse testis (lane 4, 15 μg) using Cystatin C Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Cystatin C Antibody (YA480)
    Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using Cystatin C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80639, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Cystatin C Antibody (YA480)
    Immunohistochemical analysis of paraffin-embedded human Liver Cancer tissue using Cystatin C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80639, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Cystatin C Antibody (YA480)
    Immunohistochemical analysis of paraffin-embedded human Bladder cancer tissue using Cystatin C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80639, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Cystatin C Antibody (YA480)
    Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using Cystatin C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80639, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Cystatin C Antibody (YA480)
    Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using Cystatin C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80639, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Cystatin C Antibody (YA480)
    Immunohistochemical analysis of paraffin-embedded human Ovarian Cancer‌ tissue using Cystatin C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80639, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Cystatin C Antibody (YA480)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Prostate Cancer tissue using Cystatin C antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80639, 1:800 dilution) at room temperature for 30 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Cystatin C Antibody (YA480)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Prostate Cancer tissue using Cystatin C antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80639, 1:800 dilution) at room temperature for 30 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Cystatin C Antibody (YA480)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Prostate Cancer tissue using Cystatin C antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80639, 1:800 dilution) at room temperature for 30 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Cystatin C Antibody (YA480)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Liver Cancer tissue using Cystatin C antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80639, 1:800 dilution) at room temperature for 30 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Cystatin C Antibody (YA480)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Liver Cancer tissue using Cystatin C antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80639, 1:800 dilution) at room temperature for 30 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Cystatin C Antibody (YA480)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Liver Cancer tissue using Cystatin C antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80639, 1:800 dilution) at room temperature for 30 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

Background

  • Function

    Cystatin C is an As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity

  • Subcellular Localization

    Secreted

  • Expression


    Tissue_specificity:This protein is expressed in the saliva of the submandibular and sublingual glands, but not in the parotid gland saliva (at the protein level) . It is also expressed in various body fluids, such as cerebrospinal fluid and plasma. The highest expression levels are found in the epididymis, vas deferens, brain, thymus, and ovaries, and the lowest expression levels are found in the submandibular glands.

  • Subunit

    Homodimer

  • SwissProt ID

    P01034

  • Gene ID
    1471 [NCBI]
  • Synonyms

    CST3; Cystatin-C; Cystatin-3; Gamma-trace; Neuroendocrine basic polypeptide; Post-gamma-globulin

  • Research Field

    Tags & Cell Markers

Cystatin C Antibody (YA480) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), U-87MG (lane 2, 15 μg), BxPC-3 (lane 3, 15 μg), Mouse testis (lane 4, 15 μg) using Cystatin C Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using Cystatin C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80639, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Liver Cancer tissue using Cystatin C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80639, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Bladder cancer tissue using Cystatin C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80639, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using Cystatin C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80639, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using Cystatin C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80639, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Ovarian Cancer‌ tissue using Cystatin C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80639, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Prostate Cancer tissue using Cystatin C antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80639, 1:800 dilution) at room temperature for 30 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Prostate Cancer tissue using Cystatin C antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80639, 1:800 dilution) at room temperature for 30 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Prostate Cancer tissue using Cystatin C antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80639, 1:800 dilution) at room temperature for 30 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Liver Cancer tissue using Cystatin C antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80639, 1:800 dilution) at room temperature for 30 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Liver Cancer tissue using Cystatin C antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80639, 1:800 dilution) at room temperature for 30 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Liver Cancer tissue using Cystatin C antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80639, 1:800 dilution) at room temperature for 30 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Cystatin C Antibody (YA480) Product Image
    1/16
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Cystatin C Antibody (YA480) Product Image
  • Experimental Validation Results for Cystatin C Antibody (YA480)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), U-87MG (lane 2, 15 μg), BxPC-3 (lane 3, 15 μg), Mouse testis (lane 4, 15 μg) using Cystatin C Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Cystatin C Antibody (YA480)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using Cystatin C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80639, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Cystatin C Antibody (YA480)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Liver Cancer tissue using Cystatin C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80639, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Cystatin C Antibody (YA480)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Bladder cancer tissue using Cystatin C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80639, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Cystatin C Antibody (YA480)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using Cystatin C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80639, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Cystatin C Antibody (YA480)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using Cystatin C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80639, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Cystatin C Antibody (YA480)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Ovarian Cancer‌ tissue using Cystatin C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80639, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Cystatin C Antibody (YA480)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Prostate Cancer tissue using Cystatin C antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80639, 1:800 dilution) at room temperature for 30 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Cystatin C Antibody (YA480)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Prostate Cancer tissue using Cystatin C antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80639, 1:800 dilution) at room temperature for 30 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Cystatin C Antibody (YA480)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Prostate Cancer tissue using Cystatin C antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80639, 1:800 dilution) at room temperature for 30 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Cystatin C Antibody (YA480)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Liver Cancer tissue using Cystatin C antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80639, 1:800 dilution) at room temperature for 30 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Cystatin C Antibody (YA480)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Liver Cancer tissue using Cystatin C antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80639, 1:800 dilution) at room temperature for 30 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Cystatin C Antibody (YA480)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Liver Cancer tissue using Cystatin C antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80639, 1:800 dilution) at room temperature for 30 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Experimental Validation Results for Cystatin C Antibody (YA480)
  • Cystatin C Antibody (YA480) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), U-87MG (lane 2, 15 μg), BxPC-3 (lane 3, 15 μg), Mouse testis (lane 4, 15 μg) using Cystatin C Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80955, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Cystatin C Antibody (YA480) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Prostate Cancer tissue using Cystatin C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80639, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Cystatin C Antibody (YA480) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Liver Cancer tissue using Cystatin C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80639, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Cystatin C Antibody (YA480) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Bladder cancer tissue using Cystatin C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80639, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Cystatin C Antibody (YA480) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using Cystatin C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80639, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Cystatin C Antibody (YA480) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using Cystatin C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80639, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Cystatin C Antibody (YA480) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Ovarian Cancer‌ tissue using Cystatin C antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80639, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Cystatin C Antibody (YA480) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Prostate Cancer tissue using Cystatin C antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80639, 1:800 dilution) at room temperature for 30 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Cystatin C Antibody (YA480) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Prostate Cancer tissue using Cystatin C antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80639, 1:800 dilution) at room temperature for 30 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Cystatin C Antibody (YA480) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Prostate Cancer tissue using Cystatin C antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80639, 1:800 dilution) at room temperature for 30 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Cystatin C Antibody (YA480) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Liver Cancer tissue using Cystatin C antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80639, 1:800 dilution) at room temperature for 30 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Cystatin C Antibody (YA480) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Liver Cancer tissue using Cystatin C antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80639, 1:800 dilution) at room temperature for 30 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Cystatin C Antibody (YA480) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Liver Cancer tissue using Cystatin C antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80639, 1:800 dilution) at room temperature for 30 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Cystatin C Antibody (YA480) Product Image WB
  • Cystatin C Antibody (YA480) Product Image IHC-P
  • Cystatin C Antibody (YA480) Product Image IHC-P
  • Cystatin C Antibody (YA480) Product Image IHC-P
  • Cystatin C Antibody (YA480) Product Image IHC-P
  • Cystatin C Antibody (YA480) Product Image IHC-P
  • Cystatin C Antibody (YA480) Product Image IHC-P
  • Cystatin C Antibody (YA480) Product Image mIHC
  • Cystatin C Antibody (YA480) Product Image mIHC
  • Cystatin C Antibody (YA480) Product Image mIHC
  • Cystatin C Antibody (YA480) Product Image mIHC
  • Cystatin C Antibody (YA480) Product Image mIHC
  • Cystatin C Antibody (YA480) Product Image mIHC