USP48 Antibody (YA7808)

Cat. No.: HY-P88124: Data Sheet COA
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USP48 Antibody (YA7808) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to USP48.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

USP48 Antibody (YA7808) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to USP48.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 119 kDa;

Observed band size: 119 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q86UV5

Immunogen

Human recombinant protein fragment corresponding to amino acids 361-743 of human USP48 produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG2a

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from PC-3 (lane2(20μg), Hela (lane3(20μg), Mouse kidney tissue (lane4(20μg) and Rat kidney tissue (lane5(20μg) using USP48 Antibody (HY-P88124). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.

Background

Function：Deubiquitinase that recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of polyubiquitin precursors as well as that of ubiquitinated proteins (PubMed:16214042, PubMed:34059922). Plays a role in the regulation of NF-kappa-B activation by TNF receptor superfamily via its interactions with RELA and TRAF2. May also play a regulatory role at postsynaptic sites. Plays an important role in cell cycle progression by deubiquitinating Aurora B/AURKB and thereby extending its stability (PubMed:34445214). In the context of H.pylori infection, stabilizes nuclear RELA through deubiquitination, thereby promoting the transcriptional activity of RELA to prolong TNFAIP3 de novo synthesis. Consequently, TNFAIP3 suppresses caspase activity and apoptotic cell death (PubMed:35913642). Also functions in the modulation of the ciliary and synaptic transport as well as cytoskeleton organization, which are key for photoreceptor function and homeostasis. To achieve this, stabilizes the levels of the retinal degeneration-associated proteins ARL3 and UNC119 using distinct mechanisms (PubMed:36293380). Plays a positive role in pyroptosis by stabilizing gasdermin E/GSDME through removal of its 'Lys-48'-linked ubiquitination (PubMed:36607699)

Subcellular Localization：Cytoplasm,Nucleus,Cell projection, cilium

Expression：
Tissue_Specificity: Widely expressed. Expressed in the fetal inner ear (PubMed:34059922)

Isoforms & Post-Translational Modification：Q86UV5 has 8 isomers: Q86UV5-1: 119032 Da (predicted); Q86UV5-2: 112959 Da (predicted); Q86UV5-3: 81449 Da (predicted); Q86UV5-4: 45481 Da (predicted); Q86UV5-5: 59454 Da (predicted); Q86UV5-6: 17094 Da (predicted); Q86UV5-7: 56151 Da (predicted); Q86UV5-8: 120526 Da (predicted).

Subunit：Interacts with TRAF2 and RELA

Synonyms

RAP1GA1; USP31

Documentation

Select Batch:

Data Sheet (231 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)