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GADD45A Antibody (YA1076)

(Synonyms: DDIT1; GADD45)
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Based on 1 Customer Validation

GADD45A Antibody (YA1076) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to GADD45A.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for GADD45A Antibody (YA1076)
  • GADD45A Antibody (YA1076) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

GADD45A Antibody (YA1076) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to GADD45A.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 22 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 18 kDa
Species Reactivity Database
Immunogen

Purified recombinant human GADD45 α protein expressed in E.coli

Sensitivity

Transfected

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3103126

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for GADD45A Antibody (YA1076)
    Western blot analysis of extracts from Jurkat(lane 2(20ug) , NIH/3T3(lane 3(20ug) and HEK293(lane 4(20ug) using GADD45A Antibody (HY-P81331) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    GADD45A is an In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity. Might affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase

  • Subcellular Localization

    Nucleus

  • Expression


    Induction:By UV irradiation, X-rays, growth arrest and alkylating agents. The induction is mediated by some kinase (s) other than PKC

  • Isoforms & Post-Translational Modification

    P24522 has 2 isomers: P24522-1: 18336 Da (predicted); P24522-2: 14622 Da (predicted).

  • Subunit

    Interacts with MAPK14 (By similarity). Predominantly monomeric but also forms dimers and other oligomers as concentration increases. Interacts with GADD45GIP1. Interacts weakly with PCNA. Interacts with AURKA, likely to compete with dimerization

  • SwissProt ID

    P24522

  • Gene ID
    1647 [NCBI]
  • Synonyms

    DDIT1; GADD45

  • Research Field

    Epigenetics and Nuclear Signaling

GADD45A Antibody (YA1076) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Jurkat(lane 2(20ug) , NIH/3T3(lane 3(20ug) and HEK293(lane 4(20ug) using GADD45A Antibody (HY-P81331) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • GADD45A Antibody (YA1076) Product Image
    1/16
  • Experimental Validation Results for GADD45A Antibody (YA1076)
  • GADD45A Antibody (YA1076) Product Image
  • Experimental Validation Results for GADD45A Antibody (YA1076)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Jurkat(lane 2(20ug) , NIH/3T3(lane 3(20ug) and HEK293(lane 4(20ug) using GADD45A Antibody (HY-P81331) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for GADD45A Antibody (YA1076)
  • GADD45A Antibody (YA1076) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Jurkat(lane 2(20ug) , NIH/3T3(lane 3(20ug) and HEK293(lane 4(20ug) using GADD45A Antibody (HY-P81331) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • GADD45A Antibody (YA1076) Product Image WB