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Fatty Acid Synthase Antibody (YA766)

(Synonyms: FASN; FAS; Fatty acid synthase)
2 Cited Publications
Customer Review

Based on 2 publication(s) in Google Scholar

Fatty Acid Synthase Antibody (YA766) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Fatty Acid Synthase.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat, Monkey

  • Formulation:

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA766)
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA766)
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA766)
  • Fatty Acid Synthase Antibody (YA766) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:200 1:20

Product Details

Description

Fatty Acid Synthase Antibody (YA766) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Fatty Acid Synthase.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat, Monkey
  • Observed Molecular Weight
    Observed band size: 273 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 273 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Fatty Acid Synthase.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3662785

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA766)
    Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), Hela (lane 2, 15 μg), and 3T3 (lane 3, 15 μg) using Fatty Acid Synthase (2F9) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:500 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA766)
    Immunocytochemistry analysis of C2C12 cells labeling Fatty Acid Synthase Antibody (HY-P80668) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Fatty Acid Synthase Antibody (HY-P80668) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA766)
    Immunocytochemistry analysis of C2C12 cells labeling Fatty Acid Synthase Antibody (HY-P80668) at 1/200 dilution.Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Fatty Acid Synthase Antibody (HY-P80668) at 1/200 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Fatty Acid Synthase is a Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain; (Microbial infection) Fatty acid synthetase activity is required for SARS coronavirus-2/SARS-CoV-2 replication

  • Subcellular Localization

    Cytoplasm; Melanosome

  • Expression


    Tissue_specificity:It is widely distributed. It is significantly expressed in the brain, lungs, liver, and breast.

  • Subunit

    Homodimer which is arranged in a head to tail fashion (PubMed:17618296, PubMed:18022563, Ref.34). Interacts with CEACAM1; this interaction is insulin and phosphorylation-dependent; reduces fatty-acid synthase activity

  • SwissProt ID

    P49327

  • Gene ID
    2194 [NCBI]
  • Synonyms

    FASN; FAS; Fatty acid synthase

  • Research Field

    Cardiovascular

Fatty Acid Synthase Antibody (YA766) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), Hela (lane 2, 15 μg), and 3T3 (lane 3, 15 μg) using Fatty Acid Synthase (2F9) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:500 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of C2C12 cells labeling Fatty Acid Synthase Antibody (HY-P80668) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Fatty Acid Synthase Antibody (HY-P80668) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of C2C12 cells labeling Fatty Acid Synthase Antibody (HY-P80668) at 1/200 dilution.Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Fatty Acid Synthase Antibody (HY-P80668) at 1/200 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Fatty Acid Synthase Antibody (YA766) Product Image
    1/16
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA766)
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA766)
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA766)
  • Fatty Acid Synthase Antibody (YA766) Product Image
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA766)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), Hela (lane 2, 15 μg), and 3T3 (lane 3, 15 μg) using Fatty Acid Synthase (2F9) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:500 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA766)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of C2C12 cells labeling Fatty Acid Synthase Antibody (HY-P80668) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Fatty Acid Synthase Antibody (HY-P80668) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA766)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of C2C12 cells labeling Fatty Acid Synthase Antibody (HY-P80668) at 1/200 dilution.Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Fatty Acid Synthase Antibody (HY-P80668) at 1/200 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA766)
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA766)
  • Experimental Validation Results for Fatty Acid Synthase Antibody (YA766)
  • Fatty Acid Synthase Antibody (YA766) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), Hela (lane 2, 15 μg), and 3T3 (lane 3, 15 μg) using Fatty Acid Synthase (2F9) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:500 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Fatty Acid Synthase Antibody (YA766) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of C2C12 cells labeling Fatty Acid Synthase Antibody (HY-P80668) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Fatty Acid Synthase Antibody (HY-P80668) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Fatty Acid Synthase Antibody (YA766) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of C2C12 cells labeling Fatty Acid Synthase Antibody (HY-P80668) at 1/200 dilution.Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Fatty Acid Synthase Antibody (HY-P80668) at 1/200 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Fatty Acid Synthase Antibody (YA766) Product Image WB
  • Fatty Acid Synthase Antibody (YA766) Product Image ICC
  • Fatty Acid Synthase Antibody (YA766) Product Image ICC