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UFSP2 Antibody (YA6822)

(Synonyms: C4orf20 antibody; Chromosome 4 open reading frame 20 antibody; FLJ11200 antibody; Ufm1 specific protease 2 antibody; UFM1-specific peptidase 2 antibody; Ufm1-specific protease 2 antibody; UfSP2 antibody; UFSP2_HUMAN antibody; )
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Based on 1 Customer Validation

UFSP2 Antibody (YA6822) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to UFSP2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for UFSP2 Antibody (YA6822)
  • UFSP2 Antibody (YA6822) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:2000 1:200-1:500

Product Details

Description

UFSP2 Antibody (YA6822) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to UFSP2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 53 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 53 kDa
Immunogen

Synthetic peptide within human UFSP2 aa 420-469 / 469.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for UFSP2 Antibody (YA6822)
    Western blot analysis of extracts from HEK293T (lane2(20μg), Hela (lane3(20μg), HepG2 (lane4(20μg) and Neuro-2a (lane5(20μg) using UFSP2 Antibody (HY-P87129). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    UFSP2 is a Thiol-dependent isopeptidase that specifically cleaves UFM1, a ubiquitin-like modifier protein, from conjugated proteins, such as CD274/PD-L1, CYB5R3, DDRGK1, MRE11, RPL26/uL24, TRIP4 and RPL26/uL24. While it is also able to mediate the processing of UFM1 precursors, a prerequisite for conjugation reactions, UFSP2 mainly acts as a protein deUFMylase that mediates deconjugation of UFM1 from target proteins. Mediates deUFMylation of RPL26/uL24, a critical step to release the UFM1 ribosome E3 ligase (UREL) complex during the recycling of 60S ribosome subunits from the endoplasmic reticulum. Catalyzes deUFMylation of TRIP4, regulating intracellular nuclear receptors transactivation and thereby regulate cell proliferation and differentiation[1][2][3][4][5][6][7][8][9][10][11][12][13].

  • Subcellular Localization

    Endoplasmic reticulum,Cytoplasm,Nucleus

  • Expression


    Tissue_Specificity: Expressed in brain

  • Isoforms & Post-Translational Modification

    Q9NUQ7: 469 amino acids, molecular weight 53261 Da.

  • SwissProt ID

    Q9NUQ7

  • Gene ID
    55325 [NCBI]
  • Synonyms

    C4orf20 antibody; Chromosome 4 open reading frame 20 antibody; FLJ11200 antibody; Ufm1 specific protease 2 antibody; UFM1-specific peptidase 2 antibody; Ufm1-specific protease 2 antibody; UfSP2 antibody; UFSP2_HUMAN antibody;

UFSP2 Antibody (YA6822) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293T (lane2(20μg), Hela (lane3(20μg), HepG2 (lane4(20μg) and Neuro-2a (lane5(20μg) using UFSP2 Antibody (HY-P87129). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • UFSP2 Antibody (YA6822) Product Image
    1/16
  • Experimental Validation Results for UFSP2 Antibody (YA6822)
  • UFSP2 Antibody (YA6822) Product Image
  • Experimental Validation Results for UFSP2 Antibody (YA6822)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293T (lane2(20μg), Hela (lane3(20μg), HepG2 (lane4(20μg) and Neuro-2a (lane5(20μg) using UFSP2 Antibody (HY-P87129). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for UFSP2 Antibody (YA6822)
  • UFSP2 Antibody (YA6822) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293T (lane2(20μg), Hela (lane3(20μg), HepG2 (lane4(20μg) and Neuro-2a (lane5(20μg) using UFSP2 Antibody (HY-P87129). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • UFSP2 Antibody (YA6822) Product Image WB