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Cytokeratin 10 Antibody (YA3784)

(Synonyms: BIE; EHK; K10; KPP; BCIE; CK10)
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Based on 1 Customer Validation

Cytokeratin 10 Antibody (YA3784) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Cytokeratin 10.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, FC, ELISA

  • Reactivity :

    Human, Mouse, Monkey, Rat

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Cytokeratin 10 Antibody (YA3784)
  • Cytokeratin 10 Antibody (YA3784) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

Cytokeratin 10 Antibody (YA3784) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Cytokeratin 10.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Monkey, Rat
  • Observed Molecular Weight
    Observed band size: 59 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 59 kDa
Immunogen

Purified recombinant fragment of human KRT10 (AA: 345-454 ) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Cytokeratin 10 Antibody (YA3784)
    Western blot analysis was performed on protein extracts (25 μg) from Rat skin (lane 2, Negative), Mouse skin (lane 3), Rat stomach (lane 4), HeLa (lane 5), A549 (lane 6), and THP-1 (lane 7) using Cytokeratin 10 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    Cytokeratin 10 plays a role in the establishment of the epidermal barrier on plantar skin. Involved in the maintenance of cell layer development and keratin filament bundles in suprabasal cells of the epithelium; (Microbial infection) Acts as a mediator of S.aureus adherence to desquamated nasal epithelial cells via clfB, and hence may play a role in nasal colonization; (Microbial infection) Binds S.pneumoniae PsrP, mediating adherence of the bacteria to lung cell lines. Reduction of levels of KRT10 keratin decrease adherence, overexpression increases adherence. Neither protein has to be glycosylated for the interaction to occur

  • Subcellular Localization

    Secreted, extracellular space; Cell surface; Cytoplasm

  • Expression


    Tissue_specificity:It can be found in all cell layers above the basal layer, including the stratum corneum. It is expressed on the surface of lung cell lines (PubMed:19627498) . It is localized on the surface of exfoliated nasal epithelial cells (protein level) (PubMed:12427098) .

    Induction:Repressed in keratinocytes by all-trans retinoic acid (ATRA) , via reduction of mRNA stability

  • Subunit

    Heterotetramer of two type I and two type II keratins. Heterodimer with KRT1 (PubMed:24940650, PubMed:27595935). Two heterodimers of KRT1 and KRT10 form a heterotetramer (PubMed:27595935). The KRT10 subunit in the heterotetramer is probably disulfide-linked (PubMed:27595935). Interacts with PLEC isoform 1C, when in a heterodimer with KRT1 (PubMed:24940650)

  • SwissProt ID

    P13645

  • Gene ID
    3858 [NCBI]
  • Synonyms

    BIE; EHK; K10; KPP; BCIE; CK10

Cytokeratin 10 Antibody (YA3784) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from Rat skin (lane 2, Negative), Mouse skin (lane 3), Rat stomach (lane 4), HeLa (lane 5), A549 (lane 6), and THP-1 (lane 7) using Cytokeratin 10 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Cytokeratin 10 Antibody (YA3784) Product Image
    1/16
  • Experimental Validation Results for Cytokeratin 10 Antibody (YA3784)
  • Cytokeratin 10 Antibody (YA3784) Product Image
  • Experimental Validation Results for Cytokeratin 10 Antibody (YA3784)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from Rat skin (lane 2, Negative), Mouse skin (lane 3), Rat stomach (lane 4), HeLa (lane 5), A549 (lane 6), and THP-1 (lane 7) using Cytokeratin 10 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Cytokeratin 10 Antibody (YA3784)
  • Cytokeratin 10 Antibody (YA3784) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from Rat skin (lane 2, Negative), Mouse skin (lane 3), Rat stomach (lane 4), HeLa (lane 5), A549 (lane 6), and THP-1 (lane 7) using Cytokeratin 10 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Cytokeratin 10 Antibody (YA3784) Product Image WB