ZFP36L2 Antibody
(Synonyms: ERF2, RNF162C, TIS11D, ZFP36L2, mRNA decay activator protein ZFP36L2, Butyrate response factor 2, EGF-response factor 2, TPA-induced sequence 11d, ERF-2, ZFP36-like 2)ZFP36L2 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to ZFP36L2.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 |
Product Details
ZFP36L2 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to ZFP36L2.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 51 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 51 kDa
Synthetic peptide corresponding to the center region of human ZFP36L2.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
ZFP36L2 is a Zinc-finger RNA-binding protein that destabilizes several cytoplasmic AU-rich element (ARE)-containing mRNA transcripts by promoting their poly(A) tail removal or deadenylation, and hence provide a mechanism for attenuating protein synthesis. Acts as a 3'-untranslated region (UTR) ARE mRNA-binding adapter protein to communicate signaling events to the mRNA decay machinery. Functions by recruiting the CCR4-NOT deadenylase complex and probably other components of the cytoplasmic RNA decay machinery to the bound ARE-containing mRNAs, and hence promotes ARE-mediated mRNA deadenylation and decay processes. Binds to 3'-UTR ARE of numerous mRNAs. Promotes ARE-containing mRNA decay of the low-density lipoprotein (LDL) receptor (LDLR) mRNA in response to phorbol 12-myristate 13-acetate (PMA) treatment in a p38 MAPK-dependent manner. Positively regulates early adipogenesis by promoting ARE-mediated mRNA decay of immediate early genes (IEGs). Plays a role in mature peripheral neuron integrity by promoting ARE-containing mRNA decay of the transcriptional repressor REST mRNA. Plays a role in ovulation and oocyte meiotic maturation by promoting ARE-mediated mRNA decay of the luteinizing hormone receptor LHCGR mRNA. Acts as a negative regulator of erythroid cell differentiation: promotes glucocorticoid-induced self-renewal of erythroid cells by binding mRNAs that are induced or highly expressed during terminal erythroid differentiation and promotes their degradation, preventing erythroid cell differentiation. In association with ZFP36L1 maintains quiescence on developing B lymphocytes by promoting ARE-mediated decay of several mRNAs encoding cell cycle regulators that help B cells progress through the cell cycle, and hence ensuring accurate variable-diversity-joining (VDJ) recombination process and functional immune cell formation. Together with ZFP36L1 is also necessary for thymocyte development and prevention of T-cell acute lymphoblastic leukemia (T-ALL) transformation by promoting ARE-mediated mRNA decay of the oncogenic transcription factor NOTCH1 mRNA[1][2][3][4].
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Subcellular Localization
Nucleus; Cytoplasm
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Expression
Tissue_Specificity: Expressed mainly in the basal epidermal layer, weakly in the suprabasal epidermal layers. Expressed in epidermal keratinocytes (at protein level). Expressed in oocytes.
Induction: Up-regulated by butyrate in colorectal cancer cells. Up-regulated in keratinocytes after wounding. Up-regulated strongly by granulocyte macrophage colony-stimulating factor (GM-CSF) in keratinocytes. Up-regulated moderately by transforming growth factor (TGF-beta), epidermal growth factor (EGF), tumor necrosis factor (TNF) and fibroblast growth factor (FGF1) in keratinocytes. Up-regulated also by glucocorticoid dexamethasone in keratinocytes. -
Isoforms & Post-Translational Modification
ZFP36L2 has an amino acid length of 494, molecular weight is 51063 Da.
Phosphorylated by RPS6KA1 at Ser-490 and Ser-492 upon phorbol 12-myristate 13-acetate (PMA) treatment; this phosphorylation results in dissociation of the CCR4-NOT-deadenylase complex and induces p38 MAPK-mediated stabilization of the low-density lipoprotein (LDL) receptor (LDLR) mRNA. -
Subunit
Associates with the cytoplasmic CCR4-NOT deadenylase to trigger ARE-containing mRNA deadenylation and decay processes.
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SwissProt ID
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Synonyms
ERF2, RNF162C, TIS11D, ZFP36L2, mRNA decay activator protein ZFP36L2, Butyrate response factor 2, EGF-response factor 2, TPA-induced sequence 11d, ERF-2, ZFP36-like 2
Documentation
[1]. Hudson BP, et al. Recognition of the mRNA AU-rich element by the zinc finger domain of TIS11d. Nat Struct Mol Biol. 2004 Mar;11(3):257-64. [Content Brief]
[2]. Adachi S, et al. ZFP36L1 and ZFP36L2 control LDLR mRNA stability via the ERK-RSK pathway. Nucleic Acids Res. 2014 Sep;42(15):10037-49. [Content Brief]
[3]. Zheng W, et al. Biallelic variants in ZFP36L2 cause female infertility characterised by recurrent preimplantation embryo arrest. J Med Genet. 2022 Sep;59(9):850-857. [Content Brief]
[4]. Morgan BR, et al. A computational study of RNA binding and specificity in the tandem zinc finger domain of TIS11d. Protein Sci. 2010 Jun;19(6):1222-34. [Content Brief]
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