Phospho-Elongation Factor 2 (Thr56/Thr58) Antibody (YA2581)(PBS only)

Cat. No.: HY-P82836A: Data Sheet User Guide for Antibodies
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Phospho-Elongation Factor 2 (Thr56/Thr58) Antibody (YA2581) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Elongation Factor 2 (Thr56/Thr58).

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

사이즈		재고
50 μL		견적 받기
100 μL		견적 받기

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
제품 설명
References

제품 설명

Phospho-Elongation Factor 2 (Thr56/Thr58) Antibody (YA2581) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Elongation Factor 2 (Thr56/Thr58).

Host

Rabbit

분자량

Predicted band size: 95 kDa;

Observed band size: 95 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P13639

Gene ID

1938 [NCBI]

Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Thr56/Thr58 of human EEF2/Elongation factor 2

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Background

Function：Catalyzes the GTP-dependent ribosomal translocation step during translation elongation (PubMed:26593721). During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively (PubMed:26593721). Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (PubMed:26593721)

Subcellular Localization：Cytoplasm; Nucleus

Subunit：Binds to 80S ribosomes (PubMed:27115996, PubMed:30355441). Actively translating ribosomes show mutually exclusive binding of eIF5a (EIF5A or EIF5A2) and EEF2/eEF2 (PubMed:27115996).

Research Field

Epigenetics and Nuclear Signaling

Synonyms

EEF2; EF2; Elongation factor 2; EF-2

각종 서류

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/nci-h460-cell.html [Content Brief]