dUTPase Antibody (YA6846)

製品番号: HY-P87153: データシート SDS COA User Guide for Antibodies
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dUTPase Antibody (YA6846) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to dUTPase.

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容量	価格（税別）	在庫状況	数量
10 μL	$93	在庫あり	Estimated Time of Arrival: December 31
50 μL	$240	在庫あり	Estimated Time of Arrival: December 31
100 μL	$384	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
説明
参考文献

製品説明

dUTPase Antibody (YA6846) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to dUTPase.

Host

Rabbit

Clonality

Recombinant,Monoclonal

分子量

Predicted band size: 27 kDa;

Observed band size: 20 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P33316

Gene ID

1854 [NCBI]

Immunogen

Recombinant protein within Human dUTPase aa 153-252 / 252.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100
FC FC: Flow Cytometry	1:500-1:1000

純度	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Hela(lane 2(20ug) , A549(lane 3(20ug) and Jurkat(lane 4(20ug) using dUTPase Antibody (HY-P87153) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Catalyzes the cleavage of 2'-deoxyuridine 5'-triphosphate (dUTP) into 2'-deoxyuridine 5'-monophosphate (dUMP) and inorganic pyrophosphate and through its action efficiently prevents uracil misincorporation into DNA and at the same time provides dUMP, the substrate for de novo thymidylate biosynthesis (PubMed:17880943, PubMed:8631816, PubMed:8805593). Inhibits peroxisome proliferator-activated receptor (PPAR) activity by binding of its N-terminal to PPAR, preventing the latter's dimerization with retinoid X receptor (By similarity). Essential for embryonic development (By similarity)

Subcellular Localization：Nucleus,Mitochondrion

Expression：
Tissue_Specificity: Found in a variety of tissues. Isoform 3 expression is constitutive, while isoform 2 expression correlates with the onset of DNA replication (at protein level). Isoform 2 degradation coincides with the cessation of nuclear DNA replication (at protein level)

Isoforms & Post-Translational Modification：P33316 has two isomers: P33316-3: 26563 Da (predicted); P33316-2: 17748 Da (predicted).
Nuclear isoform 2 is phosphorylated in vivo on Ser-11, a reaction that can be catalyzed in vitro by CDC2

Subunit：Homotrimer

RRID

AB_3719272

Synonyms

Deoxyuridine 5' triphosphate nucleotidohydrolase, nuclear isoform antibody; Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial antibody; Deoxyuridine triphosphatase antibody; dut antibody; DUT_HUMAN antibody; dUTP nucleotidohydrolase antibody; dUTP pyrophosphatase antibody; dUTPase antibody; FLJ20622 antibody;

ドキュメンテーション

Select Batch:

データシート (231 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

参考文献

[1]. /biology-dictionary/baf3-cell.html [Content Brief]