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dUTPase Antibody (YA6846)

(Synonyms: Deoxyuridine 5' triphosphate nucleotidohydrolase, nuclear isoform antibody; Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial antibody; Deoxyuridine triphosphatase antibody; dut antibody; DUT_HUMAN antibody; dUTP nucleotidohydrolase antibody; dUTP pyrophosphatase antibody; dUTPase antibody; FLJ20622 antibody; )
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Based on 1 Customer Validation

dUTPase Antibody (YA6846) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to dUTPase.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for dUTPase Antibody (YA6846)
  • dUTPase Antibody (YA6846) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000-1:2000 1:1000 1:100 1:500-1:1000

Product Details

Description

dUTPase Antibody (YA6846) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to dUTPase.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 20 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 27 kDa
Immunogen

Recombinant protein within Human dUTPase aa 153-252 / 252.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719272

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for dUTPase Antibody (YA6846)
    Western blot analysis of extracts from Hela(lane 2(20ug) , A549(lane 3(20ug) and Jurkat(lane 4(20ug) using dUTPase Antibody (HY-P87153) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    dUTPase catalyzes the cleavage of 2'-deoxyuridine 5'-triphosphate (dUTP) into 2'-deoxyuridine 5'-monophosphate (dUMP) and inorganic pyrophosphate and through its action efficiently prevents uracil misincorporation into DNA and at the same time provides dUMP, the substrate for de novo thymidylate biosynthesis. Inhibits peroxisome proliferator-activated receptor (PPAR) activity by binding of its N-terminal to PPAR, preventing the latter's dimerization with retinoid X receptor. Essential for embryonic development[1][2][3].

  • Subcellular Localization

    Nucleus,Mitochondrion

  • Expression


    Tissue_Specificity: Found in a variety of tissues. Isoform 3 expression is constitutive, while isoform 2 expression correlates with the onset of DNA replication (at protein level). Isoform 2 degradation coincides with the cessation of nuclear DNA replication (at protein level)

  • Isoforms & Post-Translational Modification

    P33316 has two isomers: P33316-3: 26563 Da (predicted); P33316-2: 17748 Da (predicted).
    Nuclear isoform 2 is phosphorylated in vivo on Ser-11, a reaction that can be catalyzed in vitro by CDC2

  • Subunit

    Homotrimer

  • SwissProt ID

    P33316

  • Gene ID
    1854 [NCBI]
  • Synonyms

    Deoxyuridine 5' triphosphate nucleotidohydrolase, nuclear isoform antibody; Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial antibody; Deoxyuridine triphosphatase antibody; dut antibody; DUT_HUMAN antibody; dUTP nucleotidohydrolase antibody; dUTP pyrophosphatase antibody; dUTPase antibody; FLJ20622 antibody;

dUTPase Antibody (YA6846) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) , A549(lane 3(20ug) and Jurkat(lane 4(20ug) using dUTPase Antibody (HY-P87153) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • dUTPase Antibody (YA6846) Product Image
    1/16
  • Experimental Validation Results for dUTPase Antibody (YA6846)
  • dUTPase Antibody (YA6846) Product Image
  • Experimental Validation Results for dUTPase Antibody (YA6846)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) , A549(lane 3(20ug) and Jurkat(lane 4(20ug) using dUTPase Antibody (HY-P87153) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for dUTPase Antibody (YA6846)
  • dUTPase Antibody (YA6846) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20ug) , A549(lane 3(20ug) and Jurkat(lane 4(20ug) using dUTPase Antibody (HY-P87153) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • dUTPase Antibody (YA6846) Product Image WB