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Ly6c Antibody (YA6645)

(Synonyms: Ly 6c antibody; Ly6c protein antibody; Ly6c1 antibody; Ly6c2 antibody; Lymphocyte antigen 6 complex antibody; Lymphocyte antigen 6 complex locus C antibody; Lymphocyte antigen 6C antibody; Lymphocyte antigen 6C1 antibody; Lymphocyte antigen 6C2 antibody; Lymphocyte antigen Ly 6C antibody; )
Customer Review

Based on 1 Customer Validation

Ly6c Antibody (YA6645) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ly6c.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Mouse

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Ly6c Antibody (YA6645)
  • Ly6c Antibody (YA6645) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:1000

Product Details

Description

Ly6c Antibody (YA6645) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ly6c.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Mouse
  • Observed Molecular Weight
    Observed band size: 14 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 14 kDa
Immunogen

Synthetic peptide within mouse Ly6c1 aa 51-100 / 131.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719233

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Ly6c Antibody (YA6645)
    Western blot analysis of extracts from Mouse lung tissue(lane 2(20μg) , Mouse brain tissue(lane 3(20μg) ,Mouse kidney tissue(lane 4(20μg)and Mouse heart tissue( lane 5(20μg) using Ly6c Antibody (HY-P86952). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

Background

  • Subcellular Localization

    Cell membrane

  • Expression


    Positive sample: Mouse lung tissue lysate, mouse kidney tissue lysate, mouse heart tissue lysate.

  • Isoforms & Post-Translational Modification

    P0CW02: 131 amino acids, molecular weight 14193 Da.

  • SwissProt ID

    P0CW02

  • Gene ID
    17067 [NCBI]
  • Synonyms

    Ly 6c antibody; Ly6c protein antibody; Ly6c1 antibody; Ly6c2 antibody; Lymphocyte antigen 6 complex antibody; Lymphocyte antigen 6 complex locus C antibody; Lymphocyte antigen 6C antibody; Lymphocyte antigen 6C1 antibody; Lymphocyte antigen 6C2 antibody; Lymphocyte antigen Ly 6C antibody;

Ly6c Antibody (YA6645) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Mouse lung tissue(lane 2(20μg) , Mouse brain tissue(lane 3(20μg) ,Mouse kidney tissue(lane 4(20μg)and Mouse heart tissue( lane 5(20μg) using Ly6c Antibody (HY-P86952). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

  • Ly6c Antibody (YA6645) Product Image
    1/16
  • Experimental Validation Results for Ly6c Antibody (YA6645)
  • Ly6c Antibody (YA6645) Product Image
  • Experimental Validation Results for Ly6c Antibody (YA6645)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Mouse lung tissue(lane 2(20μg) , Mouse brain tissue(lane 3(20μg) ,Mouse kidney tissue(lane 4(20μg)and Mouse heart tissue( lane 5(20μg) using Ly6c Antibody (HY-P86952). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Ly6c Antibody (YA6645)
  • Ly6c Antibody (YA6645) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Mouse lung tissue(lane 2(20μg) , Mouse brain tissue(lane 3(20μg) ,Mouse kidney tissue(lane 4(20μg)and Mouse heart tissue( lane 5(20μg) using Ly6c Antibody (HY-P86952). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

  • Ly6c Antibody (YA6645) Product Image WB