Peroxiredoxin 5 Antibody (YA1591)

Cat. No.: HY-P81846: Data Sheet COA
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Peroxiredoxin 5 Antibody (YA1591) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Peroxiredoxin 5.

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10 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
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Peroxiredoxin 5 Antibody (YA1591) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
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Peroxiredoxin 5 Antibody (YA1591) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Peroxiredoxin 5.

Host

Rabbit

Clonality

Recombinant, Monoclonal

분자량

Predicted band size: 22 kDa;

Observed band size: 17 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P30044

Gene ID

25824 [NCBI]

Immunogen

A synthetic peptide of human Peroxiredoxin 5

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IP IP: Immunoprecipitation	1:20

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

ALL WB IHC-P

Western blot analysis of extracts from HepG2 (lane 2(20μg) and HepG2 (lane 3 (40μg) using Peroxiredoxin 5 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using Peroxiredoxin 5 Antibody (YA1591). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81846, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using Peroxiredoxin 5 Antibody (YA1591). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81846, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

Function：Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events

Subcellular Localization：Mitochondrion; Cytoplasm; Peroxisome matrix

Expression：
Tissue_specificity:Widely expressed

Isoforms & Post-Translational Modification：P30044 has 4 isomers: P30044-1: 22086 Da (predicted); P30044-2: 17031 Da (predicted); P30044-3: 17454 Da (predicted); P30044-4: 12897 Da (predicted).
S-palmitoylated (PubMed:31740833). Palmitoylation occurs on the active site, inhibiting its reactivity; therefore PRDX5 palmitoylation status determines its antioxidant capacity (PubMed:31740833);S-palmitoylated (PubMed:31740833). Depalmitoylated by ABHD10 (PubMed:31740833)

Subunit：Monomer

RRID

AB_3103945

Database

Entrez Gene: 25824 Human ; 54683 Mouse ; 113898 Rat

SwissProt: P30044 Human ; P99029 Mouse ; Q9R063 Rat

OMIM: 606583 Human

Research Field

Cell Biology

Synonyms

ACR1; AOEB166; B166; Peroxiredoxin V; Peroxiredoxin-5; PLP; PMP20; PRDX5; PRDX6; Prx-V; PRXV; SBBI10; Thioredoxin reductase

각종 서류

Select Batch:

Data Sheet (234 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Peroxiredoxin 5 Antibody (YA1591) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Peroxiredoxin 5 Antibody (YA1591)

Peroxiredoxin 5 Antibody (YA1591) Featured Recommendations:

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Peroxiredoxin 5 Antibody (YA1591) Related Classifications

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