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JNK1 Antibody (YA720)(PBS only)

(Synonyms: AI849689 antibody; c Jun N terminal kinase 1 antibody; C-JUN kinase 1 antibody; c-Jun N-terminal kinase 1 antibody; EC 2.7.11.24 antibody; JNK 1 antibody; JNK antibody; JNK-46 antibody; JNK1A2 antibody; JNK21B1/2 antibody; MAP kinase 8 antibody; MAPK 8 antibody; mapk8 antibody; Mitogen activated protein kinase 8 antibody; MK08_HUMAN antibody; p54 gamma antibody; Prkm8 antibody; Protein kinase JNK1 antibody; Protein kinase, mitogen-activated, 8 antibody; SAPK 1 antibody; SAPK gamma antibody; SAPK1 antibody; Stress-activated protein kinase 1 antibody)

JNK1 Antibody (YA720) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to JNK1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for JNK1 Antibody (YA720)(PBS only)
  • JNK1 Antibody (YA720)(PBS only) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

JNK1 Antibody (YA720) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to JNK1.

  • Host Mouse
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 46/54 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 48 kDa
Immunogen

Synthetic peptide corresponding to Human JNK1.The exact sequence is proprietary to MCE.

Purification

affinity purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for JNK1 Antibody (YA720)(PBS only)
    Western blot analysis of extracts from C6(lane 2(20μg))、NIH-3T3 (lane 3(20μg)) and Hela(lane 4(20μg)), using JNK1  (HY-P80729) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80729, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    c-Jun N-terminal kinase 1 (JNK1) is a mitogen-activated protein kinase (MAPK) that regulates autophagy, apoptosis, and cell proliferation across diverse tissues[1][2]. Mechanistically, JNK1 integrates stress signals from cytokines, reactive oxygen species, and metabolic cues to modulate downstream transcription factors and cytoplasmic substrates, influencing cell survival and differentiation[3][4]. In oligodendrocyte progenitor cells, JNK1 controls branching architecture, proliferation, and myelination, indicating a critical role in central nervous system development[5]. Compared with JNK2 and JNK3, JNK1 uniquely mediates inflammatory responses in arthritis and contributes to hepatic steatosis under high-fat diet conditions[6][7]. In disease models, JNK1 inhibition reduces hypoxia-induced autophagy and sensitizes cancer cells to chemotherapeutic agents, demonstrating isoform-specific therapeutic potential[8]. The development of JNK1-selective inhibitors remains challenging due to conserved ATP-binding sites among JNK isoforms; however, computational approaches using molecular descriptors have been applied to predict candidate inhibitors targeting autophagy regulation in cancer[1]. Collectively, JNK1 functions as a central regulator of stress signaling, with distinct contributions to metabolic, inflammatory, and neurological disease models, setting it apart from its isoform counterparts and informing experimental design for isoform-specific modulation[1][2][6][7][8].

  • Subcellular Localization

    Cytoplasm; Nucleus; Synapse

  • Isoforms & Post-Translational Modification

    P45983 has 5 isomers: P45983-1: 48296 Da (predicted); P45983-2: 44229 Da (predicted); P45983-3: 44022 Da (predicted); P45983-4: 48088 Da (predicted); P45983-5: 35333 Da (predicted).
    Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K7 and MAP2K4, which activates the enzyme (PubMed:11062067). Phosphorylated by TAOK2 (PubMed:17158878). May be phosphorylated at Thr-183 and Tyr-185 by MAP3K1/MEKK1 (PubMed:17761173). Phosphorylated form is more concentrated at synapses than none-phosphorylated (By similarity)

  • Subunit

    Forms a complex with MAPK8IP1 and ARHGEF28 (By similarity). Found in a complex with SH3RF1, RAC1, MAP3K11/MLK3, MAP2K7/MKK7 and MAPK8IP1/JIP1.

  • SwissProt ID

    P45983

  • Gene ID
    5599 [NCBI]
  • Synonyms

    AI849689 antibody; c Jun N terminal kinase 1 antibody; C-JUN kinase 1 antibody; c-Jun N-terminal kinase 1 antibody; EC 2.7.11.24 antibody; JNK 1 antibody; JNK antibody; JNK-46 antibody; JNK1A2 antibody; JNK21B1/2 antibody; MAP kinase 8 antibody; MAPK 8 antibody; mapk8 antibody; Mitogen activated protein kinase 8 antibody; MK08_HUMAN antibody; p54 gamma antibody; Prkm8 antibody; Protein kinase JNK1 antibody; Protein kinase, mitogen-activated, 8 antibody; SAPK 1 antibody; SAPK gamma antibody; SAPK1 antibody; Stress-activated protein kinase 1 antibody

  • Research Field

    Signal Transduction

References

[1]. Chetna Kumari, et al. Predicting JNK1 Inhibitors Regulating Autophagy in Cancer using Random Forest Classifier. bioRxiv November 01, 2018

[2]. Kumar A, et al. JNK pathway signaling: a novel and smarter therapeutic targets for various biological diseases. Future Med Chem. 2015;7(15):2065-86. [Content Brief]

[3]. Seki E, et al. A liver full of JNK: signaling in regulation of cell function and disease pathogenesis, and clinical approaches. Gastroenterology. 2012 Aug;143(2):307-20. [Content Brief]

[4]. Shashikanth N, et al. Role of C-Jun N-Terminal Kinases on a Stressed Epithelium: Time for Testing Isoform Specificity. Biology (Basel). 2025 Jun 3;14(6):649. [Content Brief]

[5]. Lorenzati M, et al. c-Jun N-terminal kinase 1 (JNK1) modulates oligodendrocyte progenitor cell architecture, proliferation and myelination. Sci Rep. 2021 Mar 31;11(1):7264. [Content Brief]

[6]. Singh R, et al. Differential effects of JNK1 and JNK2 inhibition on murine steatohepatitis and insulin resistance. Hepatology. 2009 Jan;49(1):87-96. [Content Brief]

[7]. Denninger K, et al. JNK1, but not JNK2, is required in two mechanistically distinct models of inflammatory arthritis. Am J Pathol. 2011 Oct;179(4):1884-93. [Content Brief]

[8]. Vasilevskaya IA, et al. JNK1 Inhibition Attenuates Hypoxia-Induced Autophagy and Sensitizes to Chemotherapy. Mol Cancer Res. 2016 Aug;14(8):753-63. [Content Brief]

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JNK1 Antibody (YA720)(PBS only) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from C6(lane 2(20μg))、NIH-3T3 (lane 3(20μg)) and Hela(lane 4(20μg)), using JNK1  (HY-P80729) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80729, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • JNK1 Antibody (YA720)(PBS only) Product Image
    1/16
  • Experimental Validation Results for JNK1 Antibody (YA720)(PBS only)
  • JNK1 Antibody (YA720)(PBS only) Product Image
  • Experimental Validation Results for JNK1 Antibody (YA720)(PBS only)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from C6(lane 2(20μg))、NIH-3T3 (lane 3(20μg)) and Hela(lane 4(20μg)), using JNK1  (HY-P80729) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80729, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for JNK1 Antibody (YA720)(PBS only)
  • JNK1 Antibody (YA720)(PBS only) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from C6(lane 2(20μg))、NIH-3T3 (lane 3(20μg)) and Hela(lane 4(20μg)), using JNK1  (HY-P80729) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80729, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • JNK1 Antibody (YA720)(PBS only) Product Image WB