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RIPK2 Antibody (YA6745)

(Synonyms: CARD 3 antibody; CARD carrying kinase antibody; CARD containing ICE associated kinase antibody; CARD containing IL 1 beta ICE kinase antibody; CARD containing IL1 beta ICE kinase antibody; CARD containing interleukin 1 beta converting enzyme (ICE) associated kinase antibody; CARD containing interleukin 1 beta converting enzyme associated kinase antibody; CARD-containing IL-1 beta ICE-kinase antibody; CARD-containing interleukin-1 beta-converting enzyme-associated kinase antibody; CARD3 antibody; CARD 3 antibody; CARD carrying kinase antibody; CARD containing ICE associated kinase antibody; CARD containing IL 1 beta ICE kinase antibody; CARD containing IL1 beta ICE kinase antibody; CARD containing interleukin 1 beta converting enzyme (ICE) associated kinase antibody; CARD containing interleukin 1 beta converting enzyme associated kinase antibody; CARD-containing IL-1 beta ICE-kinase antibody; CARD-containing interleukin-1 beta-converting enzyme-associated kinase antibody; CARD3 antibody; CARDIAK antibody; CCK antibody; CLARP kinase antibody; GIG 30 antibody; GIG30 antibody; Growth inhibiting gene 30 antibody; Receptor interacting protein (RIP) like interacting caspase like apoptosis regulatory protein (CLARP) kinase antibody; Receptor interacting protein 2 antibody; Receptor interacting serine threonine kinase 2 antibody; Receptor interacting serine/threonine protein kinase 2 antibody; Receptor-interacting protein 2 antibody; Receptor-interacting serine/threonine-protein kinase 2 antibody; RICK antibody; R; IP : 2 antibody; R; IP : like interacting CLARP kinase antibody; RIP-2 antibody; RIP-like-interacting CLARP kinase antibody; RIPK 2 antibody; Ripk2 antibody; RIPK2_HUMAN antibody; TNFRSF antibody; Tyrosine-protein kinase RIPK2 antibody; UNQ277/PRO314/PRO34092 antibody; )
Customer Review

Based on 1 Customer Validation

RIPK2 Antibody (YA6745) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RIPK2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for RIPK2 Antibody (YA6745)
  • RIPK2 Antibody (YA6745) Product Image
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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:1000

Product Details

Description

RIPK2 Antibody (YA6745) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RIPK2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 61 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 61 kDa
Immunogen

Synthetic peptide within human RIPK2 aa 500-540.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for RIPK2 Antibody (YA6745)
    Western blot analysis of extracts from A549 (lane 1(20μg)) 、HeLa (lane 2(20μg)) 、HEK293 (lane 3(20μg)) and THP-1 (lane 4(20μg)) using RIPK2 Antibody (HY-P87052) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    RIPK2 is an innate immune adaptor kinase that connects cytosolic NOD1/NOD2 bacterial sensing to inflammatory signal transduction[1]. Mechanistically, NOD1 and NOD2 CARD domains initiate RIPK2-CARD oligomerization, and RIPK2 filament formation supports NOD2-dependent NF-κB signaling[1][2]. This pathway also depends on XIAP-RIPK2 association, because XIAP supports RIPK2 ubiquitination, NF-κB/MAPK activation, and cytokine production downstream of NOD2[3][4]. In disease models, pharmacologic RIPK2 inhibition reduced inflammation in acute peritonitis and spontaneous Crohn disease-like ileitis, supporting RIPK2 as a practical target for inflammatory bowel disease research[6]. Compared with related RIP kinase isoforms, RIPK2 shows a distinct signaling requirement, because replacing the RIPK2 kinase domain with the RIPK4 kinase domain failed to restore NOD2 signaling in RIPK2-deficient macrophages[5]. For experimental applications, RIPK2 inhibitors such as WEHI-345, GSK583, ponatinib, and ATP-pocket-binding compounds can suppress NOD-driven signaling, with later studies emphasizing blockade of RIPK2-XIAP interaction rather than simple catalytic inhibition[4][7][8].

  • Subcellular Localization

    Cytoplasm,Cell membrane,Endoplasmic reticulum

  • Expression


    Tissue_Specificity: Detected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node

  • Isoforms & Post-Translational Modification

    O43353 has two isomers: O43353-1: 61195 Da (predicted); O43353-2: 45582 Da (predicted).
    Polyubiquitinated via both 'Lys-63'- and 'Met-1'-linked polyubiquitin following recruitment by NOD1 or NOD2, creating docking sites for downstream effectors, triggering activation of the NF-kappa-B and MAP kinases signaling (PubMed:22607974, PubMed:29452636, PubMed:30026309)丨Autophosphorylated (PubMed:16824733, PubMed:21123652, PubMed:28545134, PubMed:29452636)丨Degraded via selective autophagy following interaction with IRGM (PubMed:36221902)丨(Microbial infection) Acetylation of Ser-174, Ser-176 and Ser-178 by Yersinia YopJ prevents phosphorylation and activation, thereby promoting cell death

  • Subunit

    Interacts (via CARD domain) with NOD2 (via CARD domain) (PubMed:15044951, PubMed:17355968, PubMed:19592251, PubMed:21887730, PubMed:27812135, PubMed:30279485, PubMed:30478312)

  • SwissProt ID

    O43353

  • Gene ID
    8767 [NCBI]
  • Synonyms

    CARD 3 antibody; CARD carrying kinase antibody; CARD containing ICE associated kinase antibody; CARD containing IL 1 beta ICE kinase antibody; CARD containing IL1 beta ICE kinase antibody; CARD containing interleukin 1 beta converting enzyme (ICE) associated kinase antibody; CARD containing interleukin 1 beta converting enzyme associated kinase antibody; CARD-containing IL-1 beta ICE-kinase antibody; CARD-containing interleukin-1 beta-converting enzyme-associated kinase antibody; CARD3 antibody; CARD 3 antibody; CARD carrying kinase antibody; CARD containing ICE associated kinase antibody; CARD containing IL 1 beta ICE kinase antibody; CARD containing IL1 beta ICE kinase antibody; CARD containing interleukin 1 beta converting enzyme (ICE) associated kinase antibody; CARD containing interleukin 1 beta converting enzyme associated kinase antibody; CARD-containing IL-1 beta ICE-kinase antibody; CARD-containing interleukin-1 beta-converting enzyme-associated kinase antibody; CARD3 antibody; CARDIAK antibody; CCK antibody; CLARP kinase antibody; GIG 30 antibody; GIG30 antibody; Growth inhibiting gene 30 antibody; Receptor interacting protein (RIP) like interacting caspase like apoptosis regulatory protein (CLARP) kinase antibody; Receptor interacting protein 2 antibody; Receptor interacting serine threonine kinase 2 antibody; Receptor interacting serine/threonine protein kinase 2 antibody; Receptor-interacting protein 2 antibody; Receptor-interacting serine/threonine-protein kinase 2 antibody; RICK antibody; R; IP : 2 antibody; R; IP : like interacting CLARP kinase antibody; RIP-2 antibody; RIP-like-interacting CLARP kinase antibody; RIPK 2 antibody; Ripk2 antibody; RIPK2_HUMAN antibody; TNFRSF antibody; Tyrosine-protein kinase RIPK2 antibody; UNQ277/PRO314/PRO34092 antibody;

[1]. Gong Q, et al. Structural basis of RIP2 activation and signaling. Nat Commun. 2018 Nov 26;9(1):4993. [Content Brief]

[2]. Pellegrini E, et al. RIP2 filament formation is required for NOD2 dependent NF-κB signalling. Nat Commun. 2018 Oct 2;9(1):4043. [Content Brief]

[3]. Goncharov T, et al. Disruption of XIAP-RIP2 Association Blocks NOD2-Mediated Inflammatory Signaling. Mol Cell. 2018 Feb 15;69(4):551-565.e7. [Content Brief]

[4]. Hrdinka M, et al. Small molecule inhibitors reveal an indispensable scaffolding role of RIPK2 in NOD2 signaling. EMBO J. 2018 Sep 3;37(17):e99372. [Content Brief]

[5]. Chirieleison SM, et al. Synthetic Biology Reveals the Uniqueness of the RIP Kinase Domain. J Immunol. 2016 May 15;196(10):4291-7. [Content Brief]

[6]. Tigno-Aranjuez JT, et al. In vivo inhibition of RIPK2 kinase alleviates inflammatory disease. J Biol Chem. 2014 Oct 24;289(43):29651-64. [Content Brief]

[7]. Nachbur U, et al. A RIPK2 inhibitor delays NOD signalling events yet prevents inflammatory cytokine production. Nat Commun. 2015 Mar 17;6:6442. [Content Brief]

[8]. Haile PA, et al. The Identification and Pharmacological Characterization of 6-(tert-Butylsulfonyl)-N-(5-fluoro-1H-indazol-3-yl)quinolin-4-amine (GSK583), a Highly Potent and Selective Inhibitor of RIP2 Kinase. J Med Chem. 2016 May 26;59(10):4867-80. [Content Brief]

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RIPK2 Antibody (YA6745) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A549 (lane 1(20μg)) 、HeLa (lane 2(20μg)) 、HEK293 (lane 3(20μg)) and THP-1 (lane 4(20μg)) using RIPK2 Antibody (HY-P87052) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • RIPK2 Antibody (YA6745) Product Image
    1/16
  • Experimental Validation Results for RIPK2 Antibody (YA6745)
  • RIPK2 Antibody (YA6745) Product Image
  • Experimental Validation Results for RIPK2 Antibody (YA6745)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A549 (lane 1(20μg)) 、HeLa (lane 2(20μg)) 、HEK293 (lane 3(20μg)) and THP-1 (lane 4(20μg)) using RIPK2 Antibody (HY-P87052) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for RIPK2 Antibody (YA6745)
  • RIPK2 Antibody (YA6745) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A549 (lane 1(20μg)) 、HeLa (lane 2(20μg)) 、HEK293 (lane 3(20μg)) and THP-1 (lane 4(20μg)) using RIPK2 Antibody (HY-P87052) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • RIPK2 Antibody (YA6745) Product Image WB