Arginase-1 Antibody (YA3647)(PBS only)
(Synonyms: arginase 1)Arginase-1 Antibody (YA3647) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to Arginase-1.
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Host:
Mouse
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Isotype:
IgG2b
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Application:
WB, ICC/IF, FC, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:200-1:1000 | 1:200-1:400 | 1:10000 |
Product Details
Arginase-1 Antibody (YA3647) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to Arginase-1.
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Host Mouse
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 37 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 35 kDa
Purified recombinant fragment of human ARG1 (AA:1-322) expressed in E. Coli.
affinity purified.
Non-conjugated
IgG2b
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Arginase-1 is key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys; Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion. In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival. In humans, the immunological role in the monocytic/macrophage/dendritic cell (DC) lineage is unsure[1][2][3].
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Subcellular Localization
Cytoplasm; Cytoplasmic granule
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Expression
Tissue_specificity:In the immune system, it was initially reported to be selectively expressed in granulocytes (polymorphonuclear leukocytes [PMN]) (PubMed:15546957) . It has also been detected in macrophages of mycobacterial granulomas (PubMed:23749634) . During lung disease, it is expressed in type 2 innate lymphocytes (ILC2) (PubMed:27043409) .
Induction:By arginine or homoarginine -
Isoforms & Post-Translational Modification
P05089 has 3 isomers: P05089-1: 34735 Da (predicted); P05089-2: 35664 Da (predicted); P05089-3: 25356 Da (predicted).
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Subunit
Homotrimer (PubMed:16141327, PubMed:17469833, PubMed:17562323, PubMed:18802628, PubMed:2241902). Interacts with CMTM6 (PubMed:28813417)
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SwissProt ID
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Synonyms
arginase 1
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