Arginase-1 Antibody (YA3647)(PBS only)

Cat. No.: HY-P83950A: Data Sheet User Guide for Antibodies
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Arginase-1 Antibody (YA3647) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to Arginase-1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

Arginase-1 Antibody (YA3647) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to Arginase-1.

Host

Mouse

Molecular Weight

Predicted band size: 35 kDa;

Observed band size: 37 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P05089

Gene ID

383 [NCBI]

Immunogen

Purified recombinant fragment of human ARG1 (AA:1-322) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG2b

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys; Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion (PubMed:15546957, PubMed:16709924, PubMed:19380772). In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival (By similarity). In humans, the immunological role in the monocytic/macrophage/dendritic cell (DC) lineage is unsure

Subcellular Localization：Cytoplasm; Cytoplasmic granule

Expression：
Tissue_specificity:In the immune system, it was initially reported to be selectively expressed in granulocytes (polymorphonuclear leukocytes [PMN]) (PubMed:15546957) . It has also been detected in macrophages of mycobacterial granulomas (PubMed:23749634) . During lung disease, it is expressed in type 2 innate lymphocytes (ILC2) (PubMed:27043409) .

Induction:By arginine or homoarginine

Isoforms & Post-Translational Modification：P05089 has 3 isomers: P05089-1: 34735 Da (predicted); P05089-2: 35664 Da (predicted); P05089-3: 25356 Da (predicted).

Subunit：Homotrimer (PubMed:16141327, PubMed:17469833, PubMed:17562323, PubMed:18802628, PubMed:2241902). Interacts with CMTM6 (PubMed:28813417)

Synonyms

arginase 1

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/mda-mb-436-cell.html [Content Brief]