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MYL12B Antibody (YA6893)

(Synonyms: ML12B_HUMAN antibody)
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Based on 1 Customer Validation

MYL12B Antibody (YA6893) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MYL12B.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for MYL12B Antibody (YA6893)
  • MYL12B Antibody (YA6893) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000 1:100 1:500-1:1000 1:1000

Product Details

Description

MYL12B Antibody (YA6893) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MYL12B.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 20 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size：20 kDa
Immunogen

Synthetic peptide within Human MYL12B aa 123-172 / 172.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MYL12B Antibody (YA6893)
    Western blot analysis of extracts from K562 (lane 1(20μg)) 、Jurkat (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using MYL12B Antibody (HY-P87205) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    MYL12B is a Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Phosphorylation triggers actin polymerization in vascular smooth muscle. Implicated in cytokinesis, receptor capping, and cell locomotion

  • Expression


    Tissue_Specificity: Ubiquitously expressed in various hematopoietic cells

  • Isoforms & Post-Translational Modification

    O14950: 172 amino acids, molecular weight 19779 Da.

  • Subunit

    Myosin is a hexamer of 2 heavy chains and 4 light chains: interacts with myosin heavy chain MYO19

  • SwissProt ID

    O14950

  • Synonyms

    ML12B_HUMAN antibody

MYL12B Antibody (YA6893) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 1(20μg)) 、Jurkat (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using MYL12B Antibody (HY-P87205) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • MYL12B Antibody (YA6893) Product Image
    1/16
  • Experimental Validation Results for MYL12B Antibody (YA6893)
  • MYL12B Antibody (YA6893) Product Image
  • Experimental Validation Results for MYL12B Antibody (YA6893)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 1(20μg)) 、Jurkat (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using MYL12B Antibody (HY-P87205) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for MYL12B Antibody (YA6893)
  • MYL12B Antibody (YA6893) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562 (lane 1(20μg)) 、Jurkat (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using MYL12B Antibody (HY-P87205) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • MYL12B Antibody (YA6893) Product Image WB