TDG Antibody (YA7006)

Cat. No.: HY-P87323: Ficha de datos COA
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TDG Antibody (YA7006) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TDG.

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Tamaño	Precio	Stock
20 μL		Pide un presupuesto y un plazo de entrega
50 μL		Pide un presupuesto y un plazo de entrega
100 μL		Pide un presupuesto y un plazo de entrega

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Descripciòn

Descripciòn

TDG Antibody (YA7006) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TDG.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Peso molecular

Predicted band size: 46 kDa;

Observed band size: 55 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q13569

Immunogen

Recombinant protein within human TGD aa 251-410.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000-1:5000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:10000

Sensitivity	Endogenous	Pureza	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Verification Image

Western blot analysis was performed on protein extracts (30 μg) from Rat lung (lane 2, Negative), Mouse lung (lane 3), Rat spleen (lane 4), Mouse spleen (lane 5), Rat brain (lane 6), and Mouse brain (lane 7) using TDG antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot^® Turbo^™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:2000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

Function：DNA glycosylase that plays a key role in active DNA demethylation: specifically recognizes and binds 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) in the context of CpG sites and mediates their excision through base-excision repair (BER) to install an unmethylated cytosine. Cannot remove 5-hydroxymethylcytosine (5hmC). According to an alternative model, involved in DNA demethylation by mediating DNA glycolase activity toward 5-hydroxymethyluracil (5hmU) produced by deamination of 5hmC. Also involved in DNA repair by acting as a thymine-DNA glycosylase that mediates correction of G/T mispairs to G/C pairs: in the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. Its role in the repair of canonical base damage is however minor compared to its role in DNA demethylation. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine

Subcellular Localization：Nucleus

Isoforms & Post-Translational Modification：Q13569: 410 amino acids, molecular weight 46053 Da.

Subunit：Homodimer

Synonyms

C JUN leucine zipper interactive protein antibody; C JUN leucine zipper interactive protein antibody; C-JUN leucine zipper interactive protein JZA-3 antibody; E130317C12Rik antibody; EC 3.2.2.29 antibody; G/T mismatch specific thymine DNA glycosylase antibody; G/T mismatch specific thymine DNA glycosylase antibody; G/T mismatch-specific thymine DNA glycosylase antibody; JZA 3 antibody; Jza1 antibody

Documentación

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Ficha de datos (231 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)