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TDG Antibody (YA7006)

(Synonyms: C JUN leucine zipper interactive protein antibody; C JUN leucine zipper interactive protein antibody; C-JUN leucine zipper interactive protein JZA-3 antibody; E130317C12Rik antibody; EC 3.2.2.29 antibody; G/T mismatch specific thymine DNA glycosylase antibody; G/T mismatch specific thymine DNA glycosylase antibody; G/T mismatch-specific thymine DNA glycosylase antibody; JZA 3 antibody; Jza1 antibody)

TDG Antibody (YA7006) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TDG.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for TDG Antibody (YA7006)
  • TDG Antibody (YA7006) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:2000-1:5000 1:10000

Product Details

Description

TDG Antibody (YA7006) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TDG.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 46 kDa
Immunogen

Recombinant protein within human TGD aa 251-410.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TDG Antibody (YA7006)
    Western blot analysis was performed on protein extracts (30 μg) from Rat lung (lane 2, Negative), Mouse lung (lane 3), Rat spleen (lane 4), Mouse spleen (lane 5), Rat brain (lane 6), and Mouse brain (lane 7) using TDG antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:2000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    TDG is a DNA glycosylase that plays a key role in active DNA demethylation: specifically recognizes and binds 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) in the context of CpG sites and mediates their excision through base-excision repair (BER) to install an unmethylated cytosine. Cannot remove 5-hydroxymethylcytosine (5hmC). According to an alternative model, involved in DNA demethylation by mediating DNA glycolase activity toward 5-hydroxymethyluracil (5hmU) produced by deamination of 5hmC. Also involved in DNA repair by acting as a thymine-DNA glycosylase that mediates correction of G/T mispairs to G/C pairs: in the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. Its role in the repair of canonical base damage is however minor compared to its role in DNA demethylation. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine

  • Subcellular Localization

    Nucleus

  • Isoforms & Post-Translational Modification

    Q13569: 410 amino acids, molecular weight 46053 Da.

  • Subunit

    Homodimer

  • SwissProt ID

    Q13569

  • Synonyms

    C JUN leucine zipper interactive protein antibody; C JUN leucine zipper interactive protein antibody; C-JUN leucine zipper interactive protein JZA-3 antibody; E130317C12Rik antibody; EC 3.2.2.29 antibody; G/T mismatch specific thymine DNA glycosylase antibody; G/T mismatch specific thymine DNA glycosylase antibody; G/T mismatch-specific thymine DNA glycosylase antibody; JZA 3 antibody; Jza1 antibody

TDG Antibody (YA7006) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (30 μg) from Rat lung (lane 2, Negative), Mouse lung (lane 3), Rat spleen (lane 4), Mouse spleen (lane 5), Rat brain (lane 6), and Mouse brain (lane 7) using TDG antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:2000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • TDG Antibody (YA7006) Product Image
    1/16
  • Experimental Validation Results for TDG Antibody (YA7006)
  • TDG Antibody (YA7006) Product Image
  • Experimental Validation Results for TDG Antibody (YA7006)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (30 μg) from Rat lung (lane 2, Negative), Mouse lung (lane 3), Rat spleen (lane 4), Mouse spleen (lane 5), Rat brain (lane 6), and Mouse brain (lane 7) using TDG antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:2000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for TDG Antibody (YA7006)
  • TDG Antibody (YA7006) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (30 μg) from Rat lung (lane 2, Negative), Mouse lung (lane 3), Rat spleen (lane 4), Mouse spleen (lane 5), Rat brain (lane 6), and Mouse brain (lane 7) using TDG antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:2000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • TDG Antibody (YA7006) Product Image WB