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NUCB1 (Nucleobindin 1) Antibody (YA8011)

(Synonyms: CALNUC; NUC)
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Based on 1 Customer Validation

NUCB1 (Nucleobindin 1) Antibody (YA8011) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NUCB1 (Nucleobindin 1).

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for NUCB1 (Nucleobindin 1) Antibody (YA8011)
  • NUCB1 (Nucleobindin 1) Antibody (YA8011) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:2000 1:150-500 1:100-250 1:100

Product Details

Description

NUCB1 (Nucleobindin 1) Antibody (YA8011) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NUCB1 (Nucleobindin 1).

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Calculated Molecular Weight Predicted band size: 53.7 kDa
Immunogen

Full length human recombinant protein of human NUCB1 produced in HEk293T cell.

Sensitivity

Endogenous

Purification

Affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Solution

  • Formulation

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for NUCB1 (Nucleobindin 1) Antibody (YA8011)
    Western blot analysis was performed on protein extracts (25 μg) from Mouse kidney (lane 2) using NUCB1 (Nucleobindin 1) antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:2000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    NUCB1 is a Major calcium-binding protein of the Golgi which may have a role in calcium homeostasis. Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates alpha subunits of guanine nucleotide-binding proteins (G proteins)

  • Subcellular Localization

    Golgi apparatus, cis-Golgi network membrane,Cytoplasm,Secreted

  • Expression


    Tissue_Specificity: Expressed both in fetal and adult heart, lung, liver, kidney and brain, and in adult skeletal muscle, placenta and pancreas

  • Isoforms & Post-Translational Modification

    Q02818: 461 amino acids, molecular weight 53879 Da.

  • Subunit

    Interacts (via GBA motif) with guanine nucleotide-binding protein G(i) alpha subunits GNAI1, GNAI2 and GNAI3 with higher affinity for GNAI1 and GNAI3 than for GNAI2

  • SwissProt ID

    Q02818

  • Synonyms

    CALNUC; NUC

NUCB1 (Nucleobindin 1) Antibody (YA8011) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from Mouse kidney (lane 2) using NUCB1 (Nucleobindin 1) antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:2000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • NUCB1 (Nucleobindin 1) Antibody (YA8011) Product Image
    1/16
  • Experimental Validation Results for NUCB1 (Nucleobindin 1) Antibody (YA8011)
  • NUCB1 (Nucleobindin 1) Antibody (YA8011) Product Image
  • Experimental Validation Results for NUCB1 (Nucleobindin 1) Antibody (YA8011)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from Mouse kidney (lane 2) using NUCB1 (Nucleobindin 1) antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:2000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for NUCB1 (Nucleobindin 1) Antibody (YA8011)
  • NUCB1 (Nucleobindin 1) Antibody (YA8011) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from Mouse kidney (lane 2) using NUCB1 (Nucleobindin 1) antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:2000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • NUCB1 (Nucleobindin 1) Antibody (YA8011) Product Image WB