Themis Antibody (YA7028)

Cat. No.: HY-P87345: Data Sheet User Guide for Antibodies
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Themis Antibody (YA7028) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Themis.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Themis Antibody (YA7028) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Themis.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 73 kDa;

Observed band size: 73 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Rat

SwissProt ID

Q8N1K5

Immunogen

Synthetic peptide within C-terminal Human Themis.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:100
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:200
FC FC: Flow Cytometry	1:50-1:100

Sensitivity	Endogenous	Purity	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk

Subcellular Localization：Cytoplasm,Nucleus

Isoforms & Post-Translational Modification：Q8N1K5 has four isomers: Q8N1K5-1: 73452 Da (predicted); Q8N1K5-2: 69464 Da (predicted); Q8N1K5-3: 62568 Da (predicted); Q8N1K5-4: 77515 Da (predicted).
Phosphorylated on Tyr residues quickly after TCR stimulation

Subunit：Interacts with PLCG1, ITK, GRB2, and LAT

Synonyms

C6orf190 antibody; C6orf207 antibody; MGC163388 antibody; Protein THEMIS antibody; SIGNALING PHOSPHOPROTEIN SPECIFIC FOR T CELLS antibody; SPOT antibody; THEMIS antibody; THMS1_HUMAN antibody; Thymocyte expressed molecule involved in selection antibody; Thymocyte selection associated antibody

Documentation

User Guide for Antibodies (1077 KB)