MTA1 Antibody (YA4233)

Cat. No.: HY-P84536: Ficha de datos User Guide for Antibodies
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MTA1 Antibody (YA4233) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to MTA1.

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Tamaño	Stock	Cantidad
20 μL	Obtener un presupuesto 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
50 μL	Obtener un presupuesto 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
100 μL	Obtener un presupuesto 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
250 μL	Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Descripciòn

Descripciòn

MTA1 Antibody (YA4233) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to MTA1.

Host

Mouse

Clonality

Monoclonal

Peso molecular

Predicted band size: 81 kDa;

Observed band size: 81 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q13330

Gene ID

9112 [NCBI]

Immunogen

Purified recombinant fragment of human MTA1 (AA: 541-715) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Pureza	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG2b

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Background

Function：Transcriptional coregulator which can act as both a transcriptional corepressor and coactivator (PubMed:16617102, PubMed:17671180, PubMed:17922032, PubMed:21965678, PubMed:24413532). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed:16428440, PubMed:28977666). In the NuRD complex, regulates transcription of its targets by modifying the acetylation status of the target chromatin and cofactor accessibility to the target DNA (PubMed:17671180). In conjunction with other components of NuRD, acts as a transcriptional corepressor of BRCA1, ESR1, TFF1 and CDKN1A (PubMed:17922032, PubMed:24413532). Acts as a transcriptional coactivator of BCAS3, and SUMO2, independent of the NuRD complex (PubMed:16617102, PubMed:17671180, PubMed:21965678). Stimulates the expression of WNT1 by inhibiting the expression of its transcriptional corepressor SIX3 (By similarity). Regulates p53-dependent and -independent DNA repair processes following genotoxic stress (PubMed:19837670). Regulates the stability and function of p53/TP53 by inhibiting its ubiquitination by COP1 and MDM2 thereby regulating the p53-dependent DNA repair (PubMed:19837670). Plays a role in the regulation of the circadian clock and is essential for the generation and maintenance of circadian rhythms under constant light and for normal entrainment of behavior to light-dark (LD) cycles (By similarity). Positively regulates the CLOCK-BMAL1 heterodimer mediated transcriptional activation of its own transcription and the transcription of CRY1 (By similarity). Regulates deacetylation of BMAL1 by regulating SIRT1 expression, resulting in derepressing CRY1-mediated transcription repression (By similarity). With TFCP2L1, promotes establishment and maintenance of pluripotency in embryonic stem cells (ESCs) and inhibits endoderm differentiation (By similarity); Binds to ESR1 and sequesters it in the cytoplasm and enhances its non-genomic responses

Subcellular Localization：Nucleus; Cytoplasm; Nucleus; Nucleus envelope; Cytoplasm; Cytoplasm, cytoskeleton

Expression：
Tissue_specificity:Widely expressed. High expression in brain, liver, kidney, and cardiac muscle, ovaries, adrenal glands and virgin mammary glands. Higher in tumors than in adjacent normal tissue from the same individual. Up-regulated in a wide variety of cancers including breast, liver, ovarian, and colorectal cancer and its expression levels are closely correlated with tumor aggressiveness and metastasis

Isoforms & Post-Translational Modification：Q13330 has 3 isomers: Q13330-1: 80786 Da (predicted); Q13330-2: 49017 Da (predicted); Q13330-3: 79192 Da (predicted).
Phosphorylation by CSNK1G2/CK1 triggered by estrogen enhances corepression of estrogen receptor (ER);Acetylation is essential for its transcriptional coactivator activity;Sumoylation positively regulates its transcriptional corepressor activity but does not affect the protein stability. Sumoylated preferentially by SUMO2 or SUMO3 than SUMO1. Sumoylation is enhanced by PIAS1/3/4 and preferentially sumoylated by SUMO2 in the presence of PIAS1/3/4. Desumoylated by SENP1;Ubiquitinated by COP1, which leads to proteasomal degradation

Subunit：Component of the nucleosome remodeling and deacetylase (NuRD) repressor complex, composed of core proteins MTA1, MTA2, MTA3, RBBP4, RBBP7, HDAC1, HDAC2, MBD2, MBD3, and peripherally associated proteins CDK2AP1, CDK2AP2, GATAD2A, GATAD2B, CHD3, CHD4 and CHD5 (PubMed:16428440, PubMed:28977666, PubMed:33283408, PubMed:9885572). The exact stoichiometry of the NuRD complex is unknown, and some subunits such as MBD2 and MBD3, GATAD2A and GATAD2B, and CHD3, CHD4 and CHD5 define mutually exclusive NuRD complexes (PubMed:16428440, PubMed:28977666, PubMed:33283408). Interacts with RBBP4; the interaction is direct (PubMed:24920672). Interacts with BMAL1 (By similarity). Interacts with CLOCK (By similarity). Interacts with COP1 (PubMed:19805145). Interacts with CSNK1G2 in the cytoplasm (PubMed:15077195). Interacts with EP300 (PubMed:16617102). Interacts with HDAC2 (PubMed:17671180, PubMed:19805145, PubMed:21965678, PubMed:24970816). Interacts with IFI16 (PubMed:24413532). Interacts with ITGB3BP/CENPR (PubMed:15254226). Interacts with MBD3L2 (PubMed:15701600). Interacts with MDM2 (PubMed:19837670). Interacts with NACC2 (PubMed:22926524). Interacts with p53/TP53 (PubMed:19837670). Interacts with PIAS1 (PubMed:21965678). Interacts with PIAS3 (PubMed:21965678). Interacts with PIAS4 (PubMed:21965678). Interacts with PWWP2A (PubMed:30327463). Interacts with PWWP2B (By similarity). Interacts with SENP1 (PubMed:21965678). Interacts with SENP2 (PubMed:21965678). Interacts with SIX3; facilitates the binding of SIX3 to the core DNA motif of SIX3 promoter (PubMed:20682799). Interacts with SUMO1 (PubMed:21965678). Interacts with SUMO2 (PubMed:21965678). Interacts with TFCP2L1; which is indispensable for TFCP2L1-mediated self-renewal-promoting effect and endoderm-inhibiting action (By similarity). Interacts with TFAP2C (PubMed:24413532). Interacts with TPR (PubMed:24970816). Interacts with UBE2I/UBC9 (PubMed:21965678)

Documentación

User Guide for Antibodies (1077 KB)