Copy Link Print Information

TAB3 Antibody (YA2401)

(Synonyms: MAP3K7IP 3; NAP1; NFkB activating protein 1; Tab3; TAK1 binding protein 3)
Customer Review

Based on 1 Customer Validation

TAB3 Antibody (YA2401) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TAB3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for TAB3 Antibody (YA2401)
  • Experimental Validation Results for TAB3 Antibody (YA2401)
  • Experimental Validation Results for TAB3 Antibody (YA2401)
  • Experimental Validation Results for TAB3 Antibody (YA2401)
  • TAB3 Antibody (YA2401) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

TAB3 Antibody (YA2401) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TAB3.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 90 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 79 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human TAB3 aa50-94.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104756

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TAB3 Antibody (YA2401)
    Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), HepG2 (lane 2, 15 μg), Hela (lane 3, 15 μg), Caco-2 (lane 4, 15 μg), RAW264.7 (lane 5, 15 μg) , and 3T3 (lane 6, 15 μg) using TAB3 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for TAB3 Antibody (YA2401)
    Western blot analysis of extracts from HepG2 (lane 2(20μg), NIH3T3 (lane 3(20μg), HEK293T (lane 4(20μg) using TAB3 (HY-P82656) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TAB3 Antibody (YA2401)
    Immunocytochemistry analysis of HepG2 cells labeling TAB3 with TAB3 antibody (HY-P82656) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with TAB3 antibody (HY-P82656) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for TAB3 Antibody (YA2401)
    Immunocytochemistry analysis of HepG2 cells labeling TAB3 with TAB3 antibody (HY-P82656) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with TAB3 antibody (HY-P82656) at 1/100 dilution in BSA for Immunol Staining at 4℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    TAB3 is an Adapter required to activate the JNK and NF-kappa-B signaling pathways through the specific recognition of 'Lys-63'-linked polyubiquitin chains by its RanBP2-type zinc finger (NZF). Acts as an adapter linking MAP3K7/TAK1 and TRAF6 to 'Lys-63'-linked polyubiquitin chains. The RanBP2-type zinc finger (NZF) specifically recognizes Lys-63'-linked polyubiquitin chains unanchored or anchored to the substrate proteins such as RIPK1/RIP1 and RIPK2: this acts as a scaffold to organize a large signaling complex to promote autophosphorylation of MAP3K7/TAK1, and subsequent activation of I-kappa-B-kinase (IKK) core complex by MAP3K7/TAK1; May be an oncogenic factor[1][2][3][4][5][6].

  • Expression


    Tissue_specificity:Widely expressed. Constitutively overexpressed in certain tumor tissues; Major transcript; Minor transcript

  • Isoforms & Post-Translational Modification

    Q8N5C8 has 2 isomers: Q8N5C8-1: 78653 Da (predicted); Q8N5C8-2: 75569 Da (predicted).
    Ubiquitinated; following IL1 stimulation or TRAF6 overexpression (PubMed:14633987). Ubiquitinated by AMFR via 'Lys-27'-linked polyubiquitination; leading to TAK1/MAP3K7 activation (PubMed:36593296);Degraded in a lysosome-dependent manner following interaction with TRIM38;Phosphorylated at Ser-506 by MAPKAPK2 and MAPKAPK3 following IL1 treatment;(Microbial infection) Methylated at Cys-692 by enteropathogenic E.coli protein NleE or S.flexneri protein OspZ: methylation disrupts zinc-binding and ability to bind 'Lys-63'-linked ubiquitin, leading to NF-kappa-B inactivation

  • Subunit

    Interacts with TAB1, TAB2, MAP3K7, TRAF2 and TRAF6 (PubMed:14633987, PubMed:14670075, PubMed:14766965). The minimal TAB3-containing complex (TAB1-MAP3K7-TAB3) appears not to contain TAB2 (PubMed:14670075). However, it seems sensible to consider that TAB2 may also join this complex and may act in a cooperative manner with TAB3 (PubMed:14670075). Interacts with DYNC2I2 (via the WD domains) (PubMed:19521662). Interacts with RBCK1 (PubMed:17449468). Binds 'Lys-63'-linked polyubiquitin chains (PubMed:22158122). Interacts with TRIM5 (PubMed:21512573). Interacts with TRIM38 (via B30.2/SPRY domain), leading to its translocation to lysosomes and degradation (PubMed:24434549). Interacts with ASB1 (PubMed:33431678)

  • SwissProt ID

    Q8N5C8

  • Gene ID
    257397 [NCBI]
  • Synonyms

    MAP3K7IP 3; NAP1; NFkB activating protein 1; Tab3; TAK1 binding protein 3

  • Research Field

    -

TAB3 Antibody (YA2401) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), HepG2 (lane 2, 15 μg), Hela (lane 3, 15 μg), Caco-2 (lane 4, 15 μg), RAW264.7 (lane 5, 15 μg) , and 3T3 (lane 6, 15 μg) using TAB3 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 2(20μg), NIH3T3 (lane 3(20μg), HEK293T (lane 4(20μg) using TAB3 (HY-P82656) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling TAB3 with TAB3 antibody (HY-P82656) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with TAB3 antibody (HY-P82656) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling TAB3 with TAB3 antibody (HY-P82656) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with TAB3 antibody (HY-P82656) at 1/100 dilution in BSA for Immunol Staining at 4℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • TAB3 Antibody (YA2401) Product Image
    1/16
  • Experimental Validation Results for TAB3 Antibody (YA2401)
  • Experimental Validation Results for TAB3 Antibody (YA2401)
  • Experimental Validation Results for TAB3 Antibody (YA2401)
  • Experimental Validation Results for TAB3 Antibody (YA2401)
  • TAB3 Antibody (YA2401) Product Image
  • Experimental Validation Results for TAB3 Antibody (YA2401)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), HepG2 (lane 2, 15 μg), Hela (lane 3, 15 μg), Caco-2 (lane 4, 15 μg), RAW264.7 (lane 5, 15 μg) , and 3T3 (lane 6, 15 μg) using TAB3 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for TAB3 Antibody (YA2401)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 2(20μg), NIH3T3 (lane 3(20μg), HEK293T (lane 4(20μg) using TAB3 (HY-P82656) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TAB3 Antibody (YA2401)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling TAB3 with TAB3 antibody (HY-P82656) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with TAB3 antibody (HY-P82656) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for TAB3 Antibody (YA2401)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling TAB3 with TAB3 antibody (HY-P82656) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with TAB3 antibody (HY-P82656) at 1/100 dilution in BSA for Immunol Staining at 4℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for TAB3 Antibody (YA2401)
  • Experimental Validation Results for TAB3 Antibody (YA2401)
  • Experimental Validation Results for TAB3 Antibody (YA2401)
  • Experimental Validation Results for TAB3 Antibody (YA2401)
  • TAB3 Antibody (YA2401) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), HepG2 (lane 2, 15 μg), Hela (lane 3, 15 μg), Caco-2 (lane 4, 15 μg), RAW264.7 (lane 5, 15 μg) , and 3T3 (lane 6, 15 μg) using TAB3 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • TAB3 Antibody (YA2401) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HepG2 (lane 2(20μg), NIH3T3 (lane 3(20μg), HEK293T (lane 4(20μg) using TAB3 (HY-P82656) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • TAB3 Antibody (YA2401) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling TAB3 with TAB3 antibody (HY-P82656) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with TAB3 antibody (HY-P82656) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • TAB3 Antibody (YA2401) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling TAB3 with TAB3 antibody (HY-P82656) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with TAB3 antibody (HY-P82656) at 1/100 dilution in BSA for Immunol Staining at 4℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • TAB3 Antibody (YA2401) Product Image WB
  • TAB3 Antibody (YA2401) Product Image WB
  • TAB3 Antibody (YA2401) Product Image ICC
  • TAB3 Antibody (YA2401) Product Image ICC