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Cubilin Antibody (YA2866)

(Synonyms: Cubilin; Cubn; IFCR; MGA1; Cubilin precursor; megaloblastic anemia 1)
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Based on 1 Customer Validation

Cubilin Antibody (YA2866) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cubilin.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Cubilin Antibody (YA2866)
  • Cubilin Antibody (YA2866) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

Cubilin Antibody (YA2866) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cubilin.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 399 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 399 kDa
Species Reactivity Database

Entrez Gene: 8029 Human

SwissProt: O60494 Human

Immunogen

A synthesized peptide derived from human Cubilin aa3350-3530.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105236

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Cubilin Antibody (YA2866)
    Immunohistochemical analysis of paraffin-embedded human kidney tissue using Cubilin Antibody (HY-P83121, 1/50). The section was pretreated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 10 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Cubilin is an Endocytic receptor which plays a role in lipoprotein, vitamin and iron metabolism by facilitating their uptake. Acts together with LRP2 to mediate endocytosis of high-density lipoproteins, GC, hemoglobin, ALB, TF and SCGB1A1. Acts together with AMN to mediate endocytosis of the CBLIF-cobalamin complex. Binds to ALB, MB, Kappa and lambda-light chains, TF, hemoglobin, GC, SCGB1A1, APOA1, high density lipoprotein, and the CBLIF-cobalamin complex. Ligand binding requires calcium. Serves as important transporter in several absorptive epithelia, including intestine, renal proximal tubules and embryonic yolk sac. May play an important role in the development of the peri-implantation embryo through internalization of APOA1 and cholesterol. Binds to LGALS3 at the maternal-fetal interface[1][2][3][4][5].

  • Subcellular Localization

    Apical cell membrane; Peripheral membrane protein; Cell membrane; Peripheral membrane protein; Membrane, coated pit; Endosome; Lysosome membrane; Peripheral membrane protein

  • Expression


    Tissue_specificity:Detected in kidney cortex (at protein level) (PubMed:9572993) . Expressed in kidney proximal tubule cells, placenta, visceral yolk-sac cells and in absorptive intestinal cells. Expressed in the epithelium of intestine and kidney

  • Subunit

    Interacts with AMN (PubMed:14576052, PubMed:20237569, PubMed:29402915, PubMed:30523278). Component of the cubam complex composed of one CUBN trimer and one AMN chain (PubMed:30523278).

  • SwissProt ID

    O60494

  • Gene ID
    8029 [NCBI]
  • Synonyms

    Cubilin; Cubn; IFCR; MGA1; Cubilin precursor; megaloblastic anemia 1

  • Research Field

    Signal Transduction

Cubilin Antibody (YA2866) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human kidney tissue using Cubilin Antibody (HY-P83121, 1/50). The section was pretreated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 10 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cubilin Antibody (YA2866) Product Image
    1/16
  • Experimental Validation Results for Cubilin Antibody (YA2866)
  • Cubilin Antibody (YA2866) Product Image
  • Experimental Validation Results for Cubilin Antibody (YA2866)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human kidney tissue using Cubilin Antibody (HY-P83121, 1/50). The section was pretreated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 10 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cubilin Antibody (YA2866)
  • Cubilin Antibody (YA2866) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using Cubilin Antibody (HY-P83121, 1/50). The section was pretreated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 10 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cubilin Antibody (YA2866) Product Image IHC-P