DDX50 Antibody (YA8178)(PBS only)

Cat. No.: HY-P88494A: Data Sheet User Guide for Antibodies
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DDX50 Antibody (YA8178) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to DDX50.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

DDX50 Antibody (YA8178) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to DDX50.

Host

Mouse

Molecular Weight

Predicted band size: 82.4 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q9BQ39

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-287 of human DDX50 produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:800-1500

Purity	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG2b

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：ATP-dependent RNA helicase that may play a role in various aspects of RNA metabolism including pre-mRNA splicing or ribosomal RNA production (PubMed:12027455). Also acts as a viral restriction factor and promotes the activation of the NF-kappa-B and IRF3 signaling pathways following its stimulation with viral RNA or infection with RNA and DNA viruses (PubMed:35215908). For instance, decreases vaccinia virus, herpes simplex virus, Zika virus or dengue virus replication during the early stage of infection (PubMed:28181036, PubMed:35215908). Mechanistically, acts via the adapter TICAM1 and independently of the DDX1-DDX21-DHX36 helicase complex to induce the production of interferon-beta (PubMed:35215908)

Subcellular Localization：Nucleus, nucleolus,Cytoplasm

Expression：
Tissue_Specificity: Highest expression in skeletal muscle, liver, heart, placenta, and kidney

Isoforms & Post-Translational Modification：Q9BQ39: 737 amino acids, molecular weight 82565 Da.

Subunit：Interacts with C1QBP

Synonyms

GU2; GUB; mcdrh; RH-II/GuB

Documentation

User Guide for Antibodies (1077 KB)