Dopamine Receptor D3 Antibody (YA1742)

Cat. No.: HY-P81997: Data Sheet COA
SDS
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Dopamine Receptor D3 Antibody (YA1742) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Dopamine Receptor D3.

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10 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
250 μL	견적 받기

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Dopamine Receptor D3 Antibody (YA1742) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
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Dopamine Receptor D3 Antibody (YA1742) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Dopamine Receptor D3.

Host

Rabbit

Clonality

Recombinant, Monoclonal

분자량

Predicted band size: 44 kDa;

Observed band size: 44 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P35462

Gene ID

1814 [NCBI]

Immunogen

A synthesized peptide derived from human Dopamine Receptor D3 aa1-50/400.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Hela(lane 2(20ug), SH-SY5Y(lane 3(20ug) and HEK293(lane 4(20ug) using Dopamine Receptor D3 Antibody (HY-P81997) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation

Subcellular Localization：Cell membrane; Multi-pass membrane protein

Expression：
Tissue_specificity:Brain

Isoforms & Post-Translational Modification：P35462 has 2 isomers: P35462-1: 44195 Da (predicted); P35462-3: 40730 Da (predicted).
Phosphorylated by GRK4 (GRK4-alpha and GRK4-gamma);Palmitoylated

Subunit：Interacts with GRK4 (PubMed:19520868). Interacts with PALM (PubMed:16386234). Interacts with FLNA (via filamin repeat 21); increases PKA-mediated phosphorylation of FLNA (PubMed:26460884)

RRID

AB_3104118

Database

Entrez Gene: 1814 Human ; 13490 Mouse ; 29238 Rat

SwissProt: P35462 Human ; P30728 Mouse ; P19020 Rat

OMIM: 190300 Human

Research Field

Neuroscience

Synonyms

D(3) dopamine receptor; Dopamine D3 receptor; DRD3; ETM1; FET1

각종 서류

Select Batch:

Data Sheet (232 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Dopamine Receptor D3 Antibody (YA1742) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Dopamine Receptor D3 Antibody (YA1742)

Dopamine Receptor D3 Antibody (YA1742) Featured Recommendations:

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Dopamine Receptor D3 Antibody (YA1742) Related Classifications

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