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Dopamine Receptor D3 Antibody (YA1742)

(Synonyms: D(3) dopamine receptor; Dopamine D3 receptor; DRD3; ETM1; FET1)
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Based on 1 Customer Validation

Dopamine Receptor D3 Antibody (YA1742) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Dopamine Receptor D3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Dopamine Receptor D3 Antibody (YA1742)
  • Dopamine Receptor D3 Antibody (YA1742) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Dopamine Receptor D3 Antibody (YA1742) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Dopamine Receptor D3.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 44 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 44 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Dopamine Receptor D3 aa1-50/400.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104118

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Dopamine Receptor D3 Antibody (YA1742)
    Western blot analysis of extracts from Hela(lane 2(20ug), SH-SY5Y(lane 3(20ug) and HEK293(lane 4(20ug) using Dopamine Receptor D3 Antibody (HY-P81997) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Dopamine Receptor D3 is a Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Brain

  • Isoforms & Post-Translational Modification

    P35462 has 2 isomers: P35462-1: 44195 Da (predicted); P35462-3: 40730 Da (predicted).
    Phosphorylated by GRK4 (GRK4-alpha and GRK4-gamma);Palmitoylated

  • Subunit

    Interacts with GRK4 (PubMed:19520868). Interacts with PALM (PubMed:16386234). Interacts with FLNA (via filamin repeat 21); increases PKA-mediated phosphorylation of FLNA (PubMed:26460884)

  • SwissProt ID

    P35462

  • Gene ID
    1814 [NCBI]
  • Synonyms

    D(3) dopamine receptor; Dopamine D3 receptor; DRD3; ETM1; FET1

  • Research Field

    Neuroscience

Dopamine Receptor D3 Antibody (YA1742) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug), SH-SY5Y(lane 3(20ug) and HEK293(lane 4(20ug) using Dopamine Receptor D3 Antibody (HY-P81997) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Dopamine Receptor D3 Antibody (YA1742) Product Image
    1/16
  • Experimental Validation Results for Dopamine Receptor D3 Antibody (YA1742)
  • Dopamine Receptor D3 Antibody (YA1742) Product Image
  • Experimental Validation Results for Dopamine Receptor D3 Antibody (YA1742)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug), SH-SY5Y(lane 3(20ug) and HEK293(lane 4(20ug) using Dopamine Receptor D3 Antibody (HY-P81997) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Dopamine Receptor D3 Antibody (YA1742)
  • Dopamine Receptor D3 Antibody (YA1742) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20ug), SH-SY5Y(lane 3(20ug) and HEK293(lane 4(20ug) using Dopamine Receptor D3 Antibody (HY-P81997) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Dopamine Receptor D3 Antibody (YA1742) Product Image WB