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Cat. No.: HY-P82518A

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TRIF Antibody (YA2263)(PBS only)

Name: TRIF Antibody (YA2263)(PBS only)
Brand: MedChemExpress
SKU: HY-P82518A
Availability: PreOrder

(Synonyms: TRIF; IIAE6; MyD88-3; PRVTIRB; TICAM-1)

TRIF Antibody (YA2263) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TRIF.

For research use only. We do not sell to patients.

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Applications

Application	WB WB: Western Blot	IP IP: Immunoprecipitation
Dilution Ratio	1:500-1:1000	1:20

Description

TRIF Antibody (YA2263) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TRIF.

Host Rabbit
Species Reactivity
Human, Mouse, Rat
Observed Molecular Weight

Observed band size: 98 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Calculated Molecular Weight Predicted band size: 76 kDa

Immunogen

Recombinant protein of human TRIF

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Appearance

Liquid
Formulation

Supplied in PBS, pH 7.4.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.

Western blot analysis of extracts from Raji(lane 2(20ug) and Raji(lane 3(40ug) using TRIF Antibody (HY-P82518) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Function

TRIF is involved in innate immunity against invading pathogens. Adapter used by TLR3, TLR4 (through TICAM2) and TLR5 to mediate NF-kappa-B and interferon-regulatory factor (IRF) activation, and to induce apoptosis. Ligand binding to these receptors results in TRIF recruitment through its TIR domain. Distinct protein-interaction motifs allow recruitment of the effector proteins TBK1, TRAF6 and RIPK1, which in turn, lead to the activation of transcription factors IRF3 and IRF7, NF-kappa-B and FADD respectively. Phosphorylation by TBK1 on the pLxIS motif leads to recruitment and subsequent activation of the transcription factor IRF3 to induce expression of type I interferon and exert a potent immunity against invading pathogens. Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of pro-inflammatory cytokines^[1]^[2]^[3]^[4]^[5]^[6].
Subcellular Localization

Cytoplasmic vesicle, autophagosome; Cytoplasm, cytosol; Mitochondrion
Expression

Tissue_specificity:Ubiquitously expressed but with higher levels in liver
Subunit

Homodimer (PubMed:12539043). Interacts with AZI2 and IRF7 (PubMed:12471095, PubMed:15611223). Interacts with TICAM2 in TLR4 recruitment (PubMed:12721283, PubMed:25736436).
SwissProt ID

Q8IUC6
Gene ID

148022 [NCBI]
Synonyms

TRIF; IIAE6; MyD88-3; PRVTIRB; TICAM-1
Research Field

Immunology