CHRNA7 Antibody (YA3981)

Cat. No.: HY-P84284: Fiche technique COA
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CHRNA7 Antibody (YA3981) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CHRNA7.

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Size	Stock	Quantité
10 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtenir un devis

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Description

Description

CHRNA7 Antibody (YA3981) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CHRNA7.

Host

Mouse

Clonality

Monoclonal

Masse moléculaire

Predicted band size: 56.4 kDa;

Observed band size: 55 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Rat

SwissProt ID

P36544

Gene ID

1139 [NCBI]

Immunogen

Purified recombinant fragment of human CHRNA7 (AA: extra 52-259) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Pureté	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Livraison

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Jurkat(lane 2(20μg) , Hela (lane 3(20μg) ,RAW264.7(lane 4(20μg)and C6( lane 5(20μg) using CHRNA7 Antibody（HY-P84284）.Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in TBST for 2 hour at room temperature. The primary antibody ( 1/750) and Loading control antibody (Beta Actin, HY-P83730，1/10000) was used in 5% nonfat powdered milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001，1/10,000) was used for 1 hour at room temperature.

Background

Function：Component of neuronal acetylcholine receptors (nAChRs) that function as pentameric, ligand-gated cation channels with high calcium permeability among other activities. nAChRs are excitatory neurotrasnmitter receptors formed by a collection of nAChR subunits known to mediate synaptic transmission in the nervous system and the neuromuscular junction. Each nAchR subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, cation permeability, and binding to allosteric modulators (PubMed:15609996, PubMed:33735609, PubMed:8145738). CHRNA7 forms homopentameric neuronal acetylcholine receptors abundantly expressed in the central nervous system, characterized by fast desensitization and high calcium permeability (PubMed:31560909, PubMed:33735609, PubMed:38382524, PubMed:8145738). Also forms heteropentamers with CHRNB2, mainly expressed in basal forebrain cholinergic neurons. Involved in the modulation of calcium-dependent signaling pathways and influences the release of neurotransmitters, including dopamine, glutamate and GABA (PubMed:33239400). Also expressed in non-neuronal cells such as immune cells like lymphocytes, monocytes and macrophages (PubMed:12508119, PubMed:16968406, PubMed:25259522). In T cells, activation induces metabotropic signaling that results in an increase of intracellular Ca2+ concentrations, independent of ionotropic receptor functions (PubMed:17709503). In macrophages, required for acetylcholine-mediated inhibition of TNF and other inflammatory cytokine release (PubMed:12508119). Once activated by acetylcholine, nicotine or other agonists, selectively inhibits production of pro-inflammatory cytokines while leaving anti-inflammatory cytokines undisturbed (PubMed:12508119, PubMed:25259522). Stimulates the cholinergic anti-inflammatory pathway, controlling inflammation by inhibiting NFKB nuclear translocation and activating the JAK2-STAT3 pathway, independently of ion channel activity (PubMed:16968406, PubMed:25259522). Also expressed in the urothelium where it modulates reflex bladder activity by increasing intracellular calcium through internal stores and decreasing basal ATP release (By similarity)

Subcellular Localization：Postsynaptic cell membrane; Multi-pass membrane protein; Cell membrane; Multi-pass membrane protein

Expression：
Tissue_specificity:Expression in neurons (PubMed: 8145738) . Expression in macrophages (protein level) (PubMed: 12508119) .

Isoforms & Post-Translational Modification：P36544 has 3 isomers: P36544-1: 56449 Da (predicted); P36544-2: 59235 Da (predicted); P36544-3: 11485 Da (predicted).
Glycosylations at Asn-46, Asn-90 and Asn-133 are essential for TMEM35A/NACHO-mediated proper subunit assembly and trafficking to the cell membrane

Subunit：Homopentamer (PubMed:33735609, PubMed:38382524). Can also form heteropentamers with CHRNB2, mainly found in basal forebrain cholinergic neurons (PubMed:33239400, PubMed:38161283). Interacts with RIC3; which is required for proper folding and assembly (PubMed:15504725, PubMed:16120769). Interacts with LYPD6 (PubMed:27344019). Interacts with CANX (PubMed:32783947)

RRID

AB_3718875

Synonyms

NACHRA7; CHRNA7-2

Documentation

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Fiche technique (235 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)