VE-Cadherin Antibody (YA3491)

Cat. No.: HY-P81097: Data Sheet COA
SDS
User Guide for Antibodies
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VE-Cadherin Antibody (YA3491) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to VE-Cadherin.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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VE-Cadherin Antibody (YA3491) Featured Recommendations:

2 Publications Citing Use of MCE VE-Cadherin Antibody (YA3491)

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

VE-Cadherin Antibody (YA3491) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to VE-Cadherin.

Host

Rabbit

Clonality

Monoclonal, Recombinant

Molecular Weight

Predicted band size: 88 kDa;

Observed band size: 130 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P33151

Gene ID

1003 [NCBI]

Immunogen

Synthetic peptide within human VECadherin aa 1-650 / 784.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100
FC FC: Flow Cytometry	1:1000

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Cadherins are calcium-dependent cell adhesion proteins (By similarity). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types (PubMed:21269602). This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions (By similarity). It associates with alpha-catenin forming a link to the cytoskeleton (PubMed:10861224). Plays a role in coupling actin fibers to cell junctions in endothelial cells, via acting as a cell junctional complex anchor for AMOTL2 and MAGI1 (By similarity). Acts in concert with KRIT1 and PALS1 to establish and maintain correct endothelial cell polarity and vascular lumen (By similarity). These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B (PubMed:20332120). Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction (PubMed:20332120). Associates with CTNND1/p120-catenin to control CADH5 endocytosis (By similarity)

Subcellular Localization：Cell junction, adherens junction; Cell membrane; Single-pass type I membrane protein; Cytoplasm

Expression：
Tissue_specificity:Expression in endothelial cells (protein level) (PubMed: 27338829) . Expression in the brain (PubMed: 2059658) .
Positive sample: EA.hy926 cell lysates, human lung tissue lysate, human kidney tissue lysate, EA.hy926.

Isoforms & Post-Translational Modification：P33151 has 2 isomers: P33151-1: 87528 Da (predicted); P33151-2: 74499 Da (predicted).
Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB (By similarity);O-glycosylated

Subunit：Part of a complex composed of AMOTL2, MAGI1 and CDH5, within the complex AMOTL2 acts as a scaffold protein for the interaction of MAGI1 with CDH5 (By similarity). The complex is required for coupling actin fibers to cell junctions in endothelial cells (By similarity). Within the complex AMOTL2 (via its N-terminus) interacts with CDH5 (By similarity). Interacts (via cadherin 5 domain) with PTPRB (By similarity). Interacts with TRPC4 (PubMed:19996314). Interacts with KRIT1 (PubMed:20332120). Interacts with PARD3 (By similarity). Interacts with RTN4 (isoform B) (PubMed:21183689). Interacts with PALS1; the interaction promotes PALS1 localization to cell junctions and is required for CDH5-mediated vascular lumen formation and endothelial cell (PubMed:27466317). Interacts with CTNND1/p120-catenin; the interaction controls CADH5 endocytosis (By similarity)

RRID

AB_3103207

Database

Entrez Gene: 1003 Human

SwissProt: P33151 Human

OMIM: 601120 Human

Synonyms

VE-cadherin; 7B4 antigen; VECD; Vascular endothelial cell cadherin; 7B 4; 7B4; 7B4 antigen; Cadherin 5; Cadherin 5 type 2; Cadherin-5; Cadherin5; CD 144; CD144 antigen; CDH 5; CDH5; CDH5 protein; Vascular endothelial cadherin; VE Cad antibody VEC; CADH5_HUMAN.

Documentation

Select Batch:

Data Sheet (235 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

VE-Cadherin Antibody (YA3491) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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VE-Cadherin Antibody (YA3491)

VE-Cadherin Antibody (YA3491) Featured Recommendations:

2 Publications Citing Use of MCE VE-Cadherin Antibody (YA3491)

Product Detail

Background

Documentation

VE-Cadherin Antibody (YA3491) Related Classifications

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