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VE-Cadherin Antibody (YA3491)

(Synonyms: VE-cadherin; 7B4 antigen; VECD; Vascular endothelial cell cadherin; 7B 4; 7B4; 7B4 antigen; Cadherin 5; Cadherin 5 type 2; Cadherin-5; Cadherin5; CD 144; CD144 antigen; CDH 5; CDH5; CDH5 protein; Vascular endothelial cadherin; VE Cad antibody VEC; CADH5_HUMAN.)
2 Cited Publications
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Based on 2 publication(s) in Google Scholar

VE-Cadherin Antibody (YA3491) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to VE-Cadherin.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for VE-Cadherin Antibody (YA3491)
  • Experimental Validation Results for VE-Cadherin Antibody (YA3491)
  • VE-Cadherin Antibody (YA3491) Product Image
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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:2000 1:100 1:1000

Product Details

Description

VE-Cadherin Antibody (YA3491) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to VE-Cadherin.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 130 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 88 kDa
Species Reactivity Database

Entrez Gene: 1003 Human

SwissProt: P33151 Human

Immunogen

Synthetic peptide within human VECadherin aa 1-650 / 784.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103207

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for VE-Cadherin Antibody (YA3491)
    Immunocytochemistry analysis of HUVEC cells labeling VE-Cadherin with VE-Cadherin Antibody (HY-P81097) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with VE-Cadherin Antibody (HY-P81097) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for VE-Cadherin Antibody (YA3491)
    Immunocytochemistry analysis of HUVEC cells labeling VE-Cadherin with VE-Cadherin Antibody (HY-P81097) at 1/200 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with VE-Cadherin Antibody (HY-P81097) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    VE-Cadherin is a Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Plays a role in coupling actin fibers to cell junctions in endothelial cells, via acting as a cell junctional complex anchor for AMOTL2 and MAGI1. Acts in concert with KRIT1 and PALS1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. Associates with CTNND1/p120-catenin to control CADH5 endocytosis[1][2][3].

  • Subcellular Localization

    Cell junction, adherens junction; Cell membrane; Single-pass type I membrane protein; Cytoplasm

  • Expression


    Tissue_specificity:Expression in endothelial cells (protein level) (PubMed: 27338829) . Expression in the brain (PubMed: 2059658) .
    Positive sample: EA.hy926 cell lysates, human lung tissue lysate, human kidney tissue lysate, EA.hy926.

  • Isoforms & Post-Translational Modification

    P33151 has 2 isomers: P33151-1: 87528 Da (predicted); P33151-2: 74499 Da (predicted).
    Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB (By similarity);O-glycosylated

  • Subunit

    Part of a complex composed of AMOTL2, MAGI1 and CDH5, within the complex AMOTL2 acts as a scaffold protein for the interaction of MAGI1 with CDH5 (By similarity). The complex is required for coupling actin fibers to cell junctions in endothelial cells (By similarity). Within the complex AMOTL2 (via its N-terminus) interacts with CDH5 (By similarity). Interacts (via cadherin 5 domain) with PTPRB (By similarity). Interacts with TRPC4 (PubMed:19996314). Interacts with KRIT1 (PubMed:20332120). Interacts with PARD3 (By similarity). Interacts with RTN4 (isoform B) (PubMed:21183689). Interacts with PALS1; the interaction promotes PALS1 localization to cell junctions and is required for CDH5-mediated vascular lumen formation and endothelial cell (PubMed:27466317). Interacts with CTNND1/p120-catenin; the interaction controls CADH5 endocytosis (By similarity)

  • SwissProt ID

    P33151

  • Gene ID
    1003 [NCBI]
  • Synonyms

    VE-cadherin; 7B4 antigen; VECD; Vascular endothelial cell cadherin; 7B 4; 7B4; 7B4 antigen; Cadherin 5; Cadherin 5 type 2; Cadherin-5; Cadherin5; CD 144; CD144 antigen; CDH 5; CDH5; CDH5 protein; Vascular endothelial cadherin; VE Cad antibody VEC; CADH5_HUMAN.

References

[1]. Brasch J, et al. Structure and binding mechanism of vascular endothelial cadherin: a divergent classical cadherin. J Mol Biol. 2011 Apr 22;408(1):57-73. [Content Brief]

[2]. Shimoyama Y, et al. Identification of three human type-II classic cadherins and frequent heterophilic interactions between different subclasses of type-II classic cadherins. Biochem J. 2000 Jul 1;349(Pt 1):159-67. [Content Brief]

[3]. Lampugnani MG, et al. CCM1 regulates vascular-lumen organization by inducing endothelial polarity. J Cell Sci. 2010 Apr 1;123(Pt 7):1073-80. [Content Brief]

VE-Cadherin Antibody (YA3491) Related Classifications

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HUVEC cells labeling VE-Cadherin with VE-Cadherin Antibody (HY-P81097) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with VE-Cadherin Antibody (HY-P81097) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HUVEC cells labeling VE-Cadherin with VE-Cadherin Antibody (HY-P81097) at 1/200 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with VE-Cadherin Antibody (HY-P81097) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • VE-Cadherin Antibody (YA3491) Product Image
    1/16
  • Experimental Validation Results for VE-Cadherin Antibody (YA3491)
  • Experimental Validation Results for VE-Cadherin Antibody (YA3491)
  • VE-Cadherin Antibody (YA3491) Product Image
  • Experimental Validation Results for VE-Cadherin Antibody (YA3491)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HUVEC cells labeling VE-Cadherin with VE-Cadherin Antibody (HY-P81097) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with VE-Cadherin Antibody (HY-P81097) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for VE-Cadherin Antibody (YA3491)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HUVEC cells labeling VE-Cadherin with VE-Cadherin Antibody (HY-P81097) at 1/200 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with VE-Cadherin Antibody (HY-P81097) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for VE-Cadherin Antibody (YA3491)
  • Experimental Validation Results for VE-Cadherin Antibody (YA3491)
  • VE-Cadherin Antibody (YA3491) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HUVEC cells labeling VE-Cadherin with VE-Cadherin Antibody (HY-P81097) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with VE-Cadherin Antibody (HY-P81097) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • VE-Cadherin Antibody (YA3491) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HUVEC cells labeling VE-Cadherin with VE-Cadherin Antibody (HY-P81097) at 1/200 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with VE-Cadherin Antibody (HY-P81097) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • VE-Cadherin Antibody (YA3491) Product Image ICC
  • VE-Cadherin Antibody (YA3491) Product Image ICC