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Kallikrein 5 Antibody (YA2889)

(Synonyms: HK5; Kallikrein 5; Kallikrein L2; KLK 5; KLK L2; SCTE)
Customer Review

Based on 1 Customer Validation

Kallikrein 5 Antibody (YA2889) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Kallikrein 5.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, FC

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for Kallikrein 5 Antibody (YA2889)
  • Experimental Validation Results for Kallikrein 5 Antibody (YA2889)
  • Experimental Validation Results for Kallikrein 5 Antibody (YA2889)
  • Kallikrein 5 Antibody (YA2889) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:200 1:50-1:100

Product Details

Description

Kallikrein 5 Antibody (YA2889) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Kallikrein 5.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 45kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 32 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Kallikrein 5 aa240-293/293.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105259

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Kallikrein 5 Antibody (YA2889)
    Western blot analysis of extracts from MCF-7 (lane2, 20μg), Mouse brain tissue (lane3, 20μg), DU145 (lane4, 20μg) and SK-BR-3 (lane5, 20μg) using Kallikrein 5 Antibody (HY-P83144). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Kallikrein 5 Antibody (YA2889)
    Immunocytochemistry analysis of MCF-7 cells labeling Kallikrein 5 with Kallikrein 5 Antibody (HY-P83144) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Kallikrein 5 Antibody (HY-P83144) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Kallikrein 5 Antibody (YA2889)
    Immunocytochemistry analysis of MCF-7 cells labeling Kallikrein 5 with Kallikrein 5 Antibody (HY-P83144) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Kallikrein 5 Antibody (HY-P83144) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Kallikrein 5 may be involved in desquamation

  • Subcellular Localization

    Secreted

  • Expression


    Tissue_specificity:Expressed in skin, breast, brain and testis. Expressed at the stratum granulosum of palmar skin

  • Subunit

    Interacts with SPINK9

  • SwissProt ID

    Q9Y337

  • Gene ID
    25818 [NCBI]
  • Synonyms

    HK5; Kallikrein 5; Kallikrein L2; KLK 5; KLK L2; SCTE

  • Research Field

    Signal Transduction

Kallikrein 5 Antibody (YA2889) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7 (lane2, 20μg), Mouse brain tissue (lane3, 20μg), DU145 (lane4, 20μg) and SK-BR-3 (lane5, 20μg) using Kallikrein 5 Antibody (HY-P83144). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling Kallikrein 5 with Kallikrein 5 Antibody (HY-P83144) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Kallikrein 5 Antibody (HY-P83144) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling Kallikrein 5 with Kallikrein 5 Antibody (HY-P83144) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Kallikrein 5 Antibody (HY-P83144) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Kallikrein 5 Antibody (YA2889) Product Image
    1/16
  • Experimental Validation Results for Kallikrein 5 Antibody (YA2889)
  • Experimental Validation Results for Kallikrein 5 Antibody (YA2889)
  • Experimental Validation Results for Kallikrein 5 Antibody (YA2889)
  • Kallikrein 5 Antibody (YA2889) Product Image
  • Experimental Validation Results for Kallikrein 5 Antibody (YA2889)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7 (lane2, 20μg), Mouse brain tissue (lane3, 20μg), DU145 (lane4, 20μg) and SK-BR-3 (lane5, 20μg) using Kallikrein 5 Antibody (HY-P83144). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Kallikrein 5 Antibody (YA2889)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling Kallikrein 5 with Kallikrein 5 Antibody (HY-P83144) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Kallikrein 5 Antibody (HY-P83144) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Kallikrein 5 Antibody (YA2889)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling Kallikrein 5 with Kallikrein 5 Antibody (HY-P83144) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Kallikrein 5 Antibody (HY-P83144) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Kallikrein 5 Antibody (YA2889)
  • Experimental Validation Results for Kallikrein 5 Antibody (YA2889)
  • Experimental Validation Results for Kallikrein 5 Antibody (YA2889)
  • Kallikrein 5 Antibody (YA2889) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from MCF-7 (lane2, 20μg), Mouse brain tissue (lane3, 20μg), DU145 (lane4, 20μg) and SK-BR-3 (lane5, 20μg) using Kallikrein 5 Antibody (HY-P83144). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Kallikrein 5 Antibody (YA2889) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling Kallikrein 5 with Kallikrein 5 Antibody (HY-P83144) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Kallikrein 5 Antibody (HY-P83144) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Kallikrein 5 Antibody (YA2889) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling Kallikrein 5 with Kallikrein 5 Antibody (HY-P83144) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Kallikrein 5 Antibody (HY-P83144) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Kallikrein 5 Antibody (YA2889) Product Image WB
  • Kallikrein 5 Antibody (YA2889) Product Image ICC
  • Kallikrein 5 Antibody (YA2889) Product Image ICC