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RRAD Antibody (YA5557)

(Synonyms: GTP-binding protein RAD; RAD1; Ras associated with diabetes; )
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Based on 1 Customer Validation

RRAD Antibody (YA5557) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RRAD.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, ICC/IF, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for RRAD Antibody (YA5557)
  • Experimental Validation Results for RRAD Antibody (YA5557)
  • Experimental Validation Results for RRAD Antibody (YA5557)
  • RRAD Antibody (YA5557) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-2000 1:100-500 1:1000-5000

Product Details

Description

RRAD Antibody (YA5557) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RRAD.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 33 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 33 kDa
Immunogen

Synthesized peptide derived from human protein. AA range: 1-100

Purification

Protein G

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3719056

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for RRAD Antibody (YA5557)
    Western blot analysis of extracts from MCF-7(lane 2(20ug), SK-OV-3(lane 3(20ug) and HepG2(lane 4(20ug),SH-SY5Y(lane 5(20ug) using RRAD Antibody(HY-P85865) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for RRAD Antibody (YA5557)
    Immunocytochemistry analysis of A431 cells labeling RRAD with RRAD Antibody (HY-P85865) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with RRAD Antibody (HY-P85865) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for RRAD Antibody (YA5557)
    Immunocytochemistry analysis of U2OS cells labeling RRAD with RRAD Antibody (HY-P85865) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with RRAD Antibody (HY-P85865) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    RRAD may regulate basal voltage-dependent L-type Ca(2+) currents and be required for beta-adrenergic augmentation of Ca(2+) influx in cardiomyocytes, thereby regulating increases in heart rate and contractile force. May play an important role in cardiac antiarrhythmia via the strong suppression of voltage-gated L-type Ca(2+) currents. Regulates voltage-dependent L-type calcium channel subunit alpha-1C trafficking to the cell membrane. Inhibits cardiac hypertrophy tH2O2gh the calmodulin-dependent kinase II (CaMKII) pathway. Inhibits phosphorylation and activation of CAMK2D[1].

  • Subcellular Localization

    Cell membrane

  • Expression


    Tissue_specificity:Most abundantly expressed in the heart. Also found in the skeletal muscle and lung. Lesser amounts in placenta and kidney. Also detected in adipose tissue. Overexpressed in muscle of type II diabetic humans

    Induction:Down-regulated in failing hearts

  • Subunit

    Interacts with calmodulin preferentially in the inactive, GDP-bound form (PubMed:18056528, PubMed:9115241). Binds CAMKII which is capable of phosphorylating RAD in vitro (PubMed:9115241). Interacts with CAMK2D (PubMed:18056528). Interacts with CACNB2; interaction may be involved in beta-adrenergic regulation of heart rate and contractile force (By similarity). Interaction with CACNB2 regulates the trafficking of CACNA1C to the cell membrane (By similarity)

  • SwissProt ID

    P55042

  • Gene ID
    6236 [NCBI]
  • Synonyms

    GTP-binding protein RAD; RAD1; Ras associated with diabetes;

RRAD Antibody (YA5557) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7(lane 2(20ug), SK-OV-3(lane 3(20ug) and HepG2(lane 4(20ug),SH-SY5Y(lane 5(20ug) using RRAD Antibody(HY-P85865) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling RRAD with RRAD Antibody (HY-P85865) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with RRAD Antibody (HY-P85865) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of U2OS cells labeling RRAD with RRAD Antibody (HY-P85865) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with RRAD Antibody (HY-P85865) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • RRAD Antibody (YA5557) Product Image
    1/16
  • Experimental Validation Results for RRAD Antibody (YA5557)
  • Experimental Validation Results for RRAD Antibody (YA5557)
  • Experimental Validation Results for RRAD Antibody (YA5557)
  • RRAD Antibody (YA5557) Product Image
  • Experimental Validation Results for RRAD Antibody (YA5557)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7(lane 2(20ug), SK-OV-3(lane 3(20ug) and HepG2(lane 4(20ug),SH-SY5Y(lane 5(20ug) using RRAD Antibody(HY-P85865) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for RRAD Antibody (YA5557)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling RRAD with RRAD Antibody (HY-P85865) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with RRAD Antibody (HY-P85865) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for RRAD Antibody (YA5557)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of U2OS cells labeling RRAD with RRAD Antibody (HY-P85865) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with RRAD Antibody (HY-P85865) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for RRAD Antibody (YA5557)
  • Experimental Validation Results for RRAD Antibody (YA5557)
  • Experimental Validation Results for RRAD Antibody (YA5557)
  • RRAD Antibody (YA5557) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from MCF-7(lane 2(20ug), SK-OV-3(lane 3(20ug) and HepG2(lane 4(20ug),SH-SY5Y(lane 5(20ug) using RRAD Antibody(HY-P85865) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • RRAD Antibody (YA5557) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A431 cells labeling RRAD with RRAD Antibody (HY-P85865) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with RRAD Antibody (HY-P85865) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • RRAD Antibody (YA5557) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of U2OS cells labeling RRAD with RRAD Antibody (HY-P85865) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with RRAD Antibody (HY-P85865) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • RRAD Antibody (YA5557) Product Image WB
  • RRAD Antibody (YA5557) Product Image ICC
  • RRAD Antibody (YA5557) Product Image ICC