RRAD Antibody (YA5557)

Cat. No.: HY-P85865: Data Sheet COA
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RRAD Antibody (YA5557) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RRAD.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

RRAD Antibody (YA5557) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RRAD.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 33 kDa;

Observed band size: 33 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P55042

Gene ID

6236 [NCBI]

Immunogen

Synthesized peptide derived from human protein. AA range: 1-100

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-500
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:1000-5000

Purity	Protein G	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from MCF-7(lane 2(20ug), SK-OV-3(lane 3(20ug) and HepG2(lane 4(20ug),SH-SY5Y(lane 5(20ug) using RRAD Antibody(HY-P85865) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：May regulate basal voltage-dependent L-type Ca(2+) currents and be required for beta-adrenergic augmentation of Ca(2+) influx in cardiomyocytes, thereby regulating increases in heart rate and contractile force (By similarity). May play an important role in cardiac antiarrhythmia via the strong suppression of voltage-gated L-type Ca(2+) currents (By similarity). Regulates voltage-dependent L-type calcium channel subunit alpha-1C trafficking to the cell membrane (By similarity). Inhibits cardiac hypertrophy tH2O2gh the calmodulin-dependent kinase II (CaMKII) pathway (PubMed:18056528). Inhibits phosphorylation and activation of CAMK2D (PubMed:18056528)

Subcellular Localization：Cell membrane

Expression：
Tissue_specificity:Most abundantly expressed in the heart. Also found in the skeletal muscle and lung. Lesser amounts in placenta and kidney. Also detected in adipose tissue. Overexpressed in muscle of type II diabetic humans

Induction:Down-regulated in failing hearts

Subunit：Interacts with calmodulin preferentially in the inactive, GDP-bound form (PubMed:18056528, PubMed:9115241). Binds CAMKII which is capable of phosphorylating RAD in vitro (PubMed:9115241). Interacts with CAMK2D (PubMed:18056528). Interacts with CACNB2; interaction may be involved in beta-adrenergic regulation of heart rate and contractile force (By similarity). Interaction with CACNB2 regulates the trafficking of CACNA1C to the cell membrane (By similarity)

RRID

AB_3719056

Synonyms

GTP-binding protein RAD; RAD1; Ras associated with diabetes;

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)