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SERCA1 ATPase Antibody (YA2157)

(Synonyms: ATP2A; ATP2A1; SERCA1)
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Based on 1 Customer Validation

SERCA1 ATPase Antibody (YA2157) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SERCA1 ATPase.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for SERCA1 ATPase Antibody (YA2157)
  • SERCA1 ATPase Antibody (YA2157) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:2000 1:100-1:200 1:50

Product Details

Description

SERCA1 ATPase Antibody (YA2157) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SERCA1 ATPase.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 100 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 110 kDa
Species Reactivity Database

Entrez Gene: 487 Human

SwissProt: O14983 Human

Immunogen

A synthesized peptide derived from human ATP2A1/SERCA1 aa1-54/1001.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for SERCA1 ATPase Antibody (YA2157)
    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using SERCA1 ATPase antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82412, 1/200) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    SERCA1 ATPase is key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction[1].

  • Subcellular Localization

    Endoplasmic reticulum membrane; Multi-pass membrane protein; Sarcoplasmic reticulum membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Skeletal muscle, fast-twitch fibers (Type II)

    Induction:Increased contractile activity leads to a decrease in SERCA1 expression, while decreased contractile activity leads to an increase in SERCA1 expression

  • Isoforms & Post-Translational Modification

    O14983 has 3 isomers: O14983-1: 110252 Da (predicted); O14983-2: 109283 Da (predicted); O14983-3: 95199 Da (predicted).

  • Subunit

    Interacts with sarcolipin (SLN) (By similarity). Interacts with phospholamban (PLN) (By similarity). Interacts with myoregulin (MRLN). Interacts with DWORF (By similarity). Interacts with VMP1 (PubMed:28890335)

  • SwissProt ID

    O14983

  • Gene ID
    487 [NCBI]
  • Synonyms

    ATP2A; ATP2A1; SERCA1

  • Research Field

    Tags & Cell Markers

SERCA1 ATPase Antibody (YA2157) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human skeletal muscle using SERCA1 ATPase antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82412, 1/200) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • SERCA1 ATPase Antibody (YA2157) Product Image
    1/16
  • Experimental Validation Results for SERCA1 ATPase Antibody (YA2157)
  • SERCA1 ATPase Antibody (YA2157) Product Image
  • Experimental Validation Results for SERCA1 ATPase Antibody (YA2157)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human skeletal muscle using SERCA1 ATPase antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82412, 1/200) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SERCA1 ATPase Antibody (YA2157)
  • SERCA1 ATPase Antibody (YA2157) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using SERCA1 ATPase antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82412, 1/200) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • SERCA1 ATPase Antibody (YA2157) Product Image IHC-P