Doublecortin Antibody (YA3541)(PBS only)

Cat. No.: HY-P83844A: Data Sheet User Guide for Antibodies
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Doublecortin Antibody (YA3541) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Doublecortin.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Doublecortin Antibody (YA3541) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Doublecortin.

Host

Mouse

Molecular Weight

Predicted band size: 41 kDa;

Observed band size: 41 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat, Rabbit, Monkey

SwissProt ID

O43602

Gene ID

1641 [NCBI]

Immunogen

Purified recombinant fragment of human DCX (AA: 362-411) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration

Subcellular Localization：Cytoplasm; Cell projection, neuron projection

Expression：
Tissue_specificity:It is highly expressed in fetal brain neurons (most cells in the cortical plate, intermediate zone, and ventricular zone) , but not in other fetal tissues. In humans, it is highly expressed in the frontal lobe of the brain, but very low in other brain regions, and is not detected in the heart, placenta, lungs, liver, skeletal muscle, kidneys, and pancreas.

Isoforms & Post-Translational Modification：O43602 has 2 isomers: O43602-1: 40574 Da (predicted); O43602-2: 40044 Da (predicted).
Phosphorylation by MARK1, MARK2 and PKA regulates its ability to bind microtubules (By similarity). Phosphorylation at Ser-265 and Ser-297 seems to occur only in neonatal brain, the levels falling precipitously by postnatal day 21 (By similarity);Ubiquitinated by MDM2, leading to its degradation by the proteasome. Ubiquitinated by MDM2 and subsequent degradation leads to reduce the dendritic spine density of olfactory bulb granule cells

Subunit：Interacts with tubulin (PubMed:27292316). Interacts with USP9X (PubMed:24607389)

Synonyms

DC; DBCN; LISX; SCLH; XLIS

Documentation

User Guide for Antibodies (1077 KB)