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Telomeric Repeat Binding Factor 2 Antibody (YA2304)

(Synonyms: Terf2; TRBF2; TRF2)
Customer Review

Based on 1 Customer Validation

Telomeric Repeat Binding Factor 2 Antibody (YA2304) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Telomeric Repeat Binding Factor 2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for Telomeric Repeat Binding Factor 2 Antibody (YA2304)
  • Experimental Validation Results for Telomeric Repeat Binding Factor 2 Antibody (YA2304)
  • Experimental Validation Results for Telomeric Repeat Binding Factor 2 Antibody (YA2304)
  • Experimental Validation Results for Telomeric Repeat Binding Factor 2 Antibody (YA2304)
  • Experimental Validation Results for Telomeric Repeat Binding Factor 2 Antibody (YA2304)
  • Experimental Validation Results for Telomeric Repeat Binding Factor 2 Antibody (YA2304)
  • Experimental Validation Results for Telomeric Repeat Binding Factor 2 Antibody (YA2304)
  • Telomeric Repeat Binding Factor 2 Antibody (YA2304) Product Image
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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200

Product Details

Description

Telomeric Repeat Binding Factor 2 Antibody (YA2304) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Telomeric Repeat Binding Factor 2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 66 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 60 kDa
Species Reactivity Database

Entrez Gene: 7014 Human

SwissProt: Q15554 Human

Immunogen

A synthesized peptide derived from human TRF2 aa50-100.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104660

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Telomeric Repeat Binding Factor 2 Antibody (YA2304)
    Western blot analysis was performed on extracts from Jurkat (lane 1, 20 μg), Daudi (lane 2, 20 μg), 293 (lane 3, 20 μg), and K562 (lane 4, 20 μg) using Telomeric Repeat Binding Factor 2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Telomeric Repeat Binding Factor 2 Antibody (YA2304)
    Immunohistochemical analysis of paraffin-embedded human kidney tissue using Telomeric Repeat Binding Factor 2 Antibody (HY-P82559, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Telomeric Repeat Binding Factor 2 Antibody (YA2304)
    Immunohistochemical analysis of paraffin-embedded human small intestine tissue using Telomeric Repeat Binding Factor 2 Antibody (HY-P82559, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Telomeric Repeat Binding Factor 2 Antibody (YA2304)
    Immunohistochemical analysis of paraffin-embedded human testis tissue using Telomeric Repeat Binding Factor 2 Antibody (HY-P82559, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Telomeric Repeat Binding Factor 2 Antibody (YA2304)
    Immunohistochemical analysis of paraffin-embedded human epididymis tissue using Telomeric Repeat Binding Factor 2 Antibody (HY-P82559, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Telomeric Repeat Binding Factor 2 Antibody (YA2304)
    Immunohistochemical analysis of paraffin-embedded human bronchus tissue using Telomeric Repeat Binding Factor 2 Antibody (HY-P82559, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Telomeric Repeat Binding Factor 2 Antibody (YA2304)
    Immunohistochemical analysis of paraffin-embedded human seminal vesicle tissue using Telomeric Repeat Binding Factor 2 Antibody (HY-P82559, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes (PubMed:15608617, PubMed:16166375, PubMed:20655466, PubMed:28216226, PubMed:9326950, PubMed:9326951, PubMed:9476899). In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo (PubMed:16166375, PubMed:20655466). Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection (PubMed:16166375). Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways (PubMed:16166375). Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair (PubMed:20655466). Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo (PubMed:20655466, PubMed:28216226). Preferentially binds to positive supercoiled DNA (PubMed:15608617, PubMed:20655466). Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology (PubMed:20655466). Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length (By similarity)

  • Subcellular Localization

    Nucleus; Chromosome, telomere

  • Expression


    Tissue_specificity:It is widely distributed. It is highly expressed in the spleen, thymus, prostate, uterus, testes, small intestine, colon, and peripheral blood leukocytes.

  • Isoforms & Post-Translational Modification

    Q15554 has 2 isomers: Q15554-3: 59594 Da (predicted); Q15554-4: 32298 Da (predicted).
    Phosphorylated upon DNA damage, most probably by ATM. Phosphorylated TERF2 is not bound to telomeric DNA, and rapidly localizes to damage sites;Methylated by PRMT1 at multiple arginines within the N-terminal Arg-rich region. Methylation may control association with telomeres

  • Subunit

    Homodimer (PubMed:15608617). Component of the shelterin complex (telosome) composed of TERF1, TERF2, TINF2, TERF2IP/RAP1, ACD and POT1 (PubMed:15316005, PubMed:15383534, PubMed:15608617, PubMed:18202258). Interacts with NBN; interaction takes place with unphosphorylated NBN during G1 phase and prevents to prevent ATM activation and non-homologous end joining repair (PubMed:10888888, PubMed:28216226). Interacts with SLX4/BTBD12 (PubMed:19596235). Interacts with DCLRE1B/Apollo and TERF2IP/RAP1; the interaction is direct (PubMed:16606622, PubMed:16730175, PubMed:16730176, PubMed:18468965, PubMed:18593705, PubMed:20655466)

  • SwissProt ID

    Q15554

  • Gene ID
    7014 [NCBI]
  • Synonyms

    Terf2; TRBF2; TRF2

  • Research Field

    Epigenetics and Nuclear Signaling

Telomeric Repeat Binding Factor 2 Antibody (YA2304) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Jurkat (lane 1, 20 μg), Daudi (lane 2, 20 μg), 293 (lane 3, 20 μg), and K562 (lane 4, 20 μg) using Telomeric Repeat Binding Factor 2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human kidney tissue using Telomeric Repeat Binding Factor 2 Antibody (HY-P82559, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human small intestine tissue using Telomeric Repeat Binding Factor 2 Antibody (HY-P82559, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human testis tissue using Telomeric Repeat Binding Factor 2 Antibody (HY-P82559, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human epididymis tissue using Telomeric Repeat Binding Factor 2 Antibody (HY-P82559, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human bronchus tissue using Telomeric Repeat Binding Factor 2 Antibody (HY-P82559, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human seminal vesicle tissue using Telomeric Repeat Binding Factor 2 Antibody (HY-P82559, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Telomeric Repeat Binding Factor 2 Antibody (YA2304) Product Image
    1/16
  • Experimental Validation Results for Telomeric Repeat Binding Factor 2 Antibody (YA2304)
  • Experimental Validation Results for Telomeric Repeat Binding Factor 2 Antibody (YA2304)
  • Experimental Validation Results for Telomeric Repeat Binding Factor 2 Antibody (YA2304)
  • Experimental Validation Results for Telomeric Repeat Binding Factor 2 Antibody (YA2304)
  • Experimental Validation Results for Telomeric Repeat Binding Factor 2 Antibody (YA2304)
  • Experimental Validation Results for Telomeric Repeat Binding Factor 2 Antibody (YA2304)
  • Experimental Validation Results for Telomeric Repeat Binding Factor 2 Antibody (YA2304)
  • Telomeric Repeat Binding Factor 2 Antibody (YA2304) Product Image
  • Experimental Validation Results for Telomeric Repeat Binding Factor 2 Antibody (YA2304)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Jurkat (lane 1, 20 μg), Daudi (lane 2, 20 μg), 293 (lane 3, 20 μg), and K562 (lane 4, 20 μg) using Telomeric Repeat Binding Factor 2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Telomeric Repeat Binding Factor 2 Antibody (YA2304)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human kidney tissue using Telomeric Repeat Binding Factor 2 Antibody (HY-P82559, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Telomeric Repeat Binding Factor 2 Antibody (YA2304)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human small intestine tissue using Telomeric Repeat Binding Factor 2 Antibody (HY-P82559, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Telomeric Repeat Binding Factor 2 Antibody (YA2304)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human testis tissue using Telomeric Repeat Binding Factor 2 Antibody (HY-P82559, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Telomeric Repeat Binding Factor 2 Antibody (YA2304)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human epididymis tissue using Telomeric Repeat Binding Factor 2 Antibody (HY-P82559, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Telomeric Repeat Binding Factor 2 Antibody (YA2304)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human bronchus tissue using Telomeric Repeat Binding Factor 2 Antibody (HY-P82559, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Telomeric Repeat Binding Factor 2 Antibody (YA2304)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human seminal vesicle tissue using Telomeric Repeat Binding Factor 2 Antibody (HY-P82559, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Telomeric Repeat Binding Factor 2 Antibody (YA2304)
  • Experimental Validation Results for Telomeric Repeat Binding Factor 2 Antibody (YA2304)
  • Experimental Validation Results for Telomeric Repeat Binding Factor 2 Antibody (YA2304)
  • Experimental Validation Results for Telomeric Repeat Binding Factor 2 Antibody (YA2304)
  • Experimental Validation Results for Telomeric Repeat Binding Factor 2 Antibody (YA2304)
  • Experimental Validation Results for Telomeric Repeat Binding Factor 2 Antibody (YA2304)
  • Experimental Validation Results for Telomeric Repeat Binding Factor 2 Antibody (YA2304)
  • Telomeric Repeat Binding Factor 2 Antibody (YA2304) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Jurkat (lane 1, 20 μg), Daudi (lane 2, 20 μg), 293 (lane 3, 20 μg), and K562 (lane 4, 20 μg) using Telomeric Repeat Binding Factor 2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Telomeric Repeat Binding Factor 2 Antibody (YA2304) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using Telomeric Repeat Binding Factor 2 Antibody (HY-P82559, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Telomeric Repeat Binding Factor 2 Antibody (YA2304) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue using Telomeric Repeat Binding Factor 2 Antibody (HY-P82559, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Telomeric Repeat Binding Factor 2 Antibody (YA2304) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human testis tissue using Telomeric Repeat Binding Factor 2 Antibody (HY-P82559, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Telomeric Repeat Binding Factor 2 Antibody (YA2304) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human epididymis tissue using Telomeric Repeat Binding Factor 2 Antibody (HY-P82559, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Telomeric Repeat Binding Factor 2 Antibody (YA2304) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human bronchus tissue using Telomeric Repeat Binding Factor 2 Antibody (HY-P82559, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Telomeric Repeat Binding Factor 2 Antibody (YA2304) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human seminal vesicle tissue using Telomeric Repeat Binding Factor 2 Antibody (HY-P82559, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Telomeric Repeat Binding Factor 2 Antibody (YA2304) Product Image WB
  • Telomeric Repeat Binding Factor 2 Antibody (YA2304) Product Image IHC-P
  • Telomeric Repeat Binding Factor 2 Antibody (YA2304) Product Image IHC-P
  • Telomeric Repeat Binding Factor 2 Antibody (YA2304) Product Image IHC-P
  • Telomeric Repeat Binding Factor 2 Antibody (YA2304) Product Image IHC-P
  • Telomeric Repeat Binding Factor 2 Antibody (YA2304) Product Image IHC-P
  • Telomeric Repeat Binding Factor 2 Antibody (YA2304) Product Image IHC-P