ATP6V1H Antibody
(Synonyms: CGI-11, ATP6V1H, V-type proton ATPase subunit H, V-ATPase subunit H, Nef-binding protein 1, Protein VMA13 homolog, V-ATPase 50/57 kDa subunits, Vacuolar proton pump subunit H, Vacuolar proton pump subunit SFD, NBP1)ATP6V1H Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to ATP6V1H.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 |
Product Details
ATP6V1H Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to ATP6V1H.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 55 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 55 kDa
Synthetic peptide corresponding to the center region of human ATP6V1H.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
ATP6V1H is a Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it promotes acidification of the extracellular environment (By similarity). The V-ATPase complex also acts as an activator for mTORC1 on lysosomal membrane by promoting the guanine nucleotide exchange factor (GEF) of the Ragulator complex, thereby enabling mTORC1 recruitment. Subunit H is essential for V-ATPase activity, but not for the assembly of the complex (By similarity). Involved in the endocytosis mediated by clathrin-coated pits, required for the formation of endosomes[1][2][3].
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Subcellular Localization
Cytoplasmic vesicle, clathrin-coated vesicle membrane
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Expression
Tissue_Specificity: Widely expressed. -
Isoforms & Post-Translational Modification
ATP6V1H has 2 isoforms, Q9UI12-1: amino acid length is 483, molecular weight is 55883 Da (predicted); Q9UI12-2: amino acid length is 465, molecular weight is 54151 Da (predicted).
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Subunit
V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex.
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SwissProt ID
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Synonyms
CGI-11, ATP6V1H, V-type proton ATPase subunit H, V-ATPase subunit H, Nef-binding protein 1, Protein VMA13 homolog, V-ATPase 50/57 kDa subunits, Vacuolar proton pump subunit H, Vacuolar proton pump subunit SFD, NBP1
Documentation
[1]. Wang L, et al. Structures of a Complete Human V-ATPase Reveal Mechanisms of Its Assembly. Mol Cell. 2020 Nov 5;80(3):501-511.e3. [Content Brief]
[2]. Zoncu R, et al. mTORC1 senses lysosomal amino acids through an inside-out mechanism that requires the vacuolar H(+)-ATPase. Science. 2011 Nov 4;334(6056):678-83. [Content Brief]
[3]. Geyer M, et al. Subunit H of the V-ATPase binds to the medium chain of adaptor protein complex 2 and connects Nef to the endocytic machinery. J Biol Chem. 2002 Aug 9;277(32):28521-9. [Content Brief]
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