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Cullin 5 Antibody (YA2585)

(Synonyms: CUL-5; VACM1; VACM-1)
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Based on 1 Customer Validation

Cullin 5 Antibody (YA2585) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cullin 5.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Cullin 5 Antibody (YA2585)
  • Cullin 5 Antibody (YA2585) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Cullin 5 Antibody (YA2585) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cullin 5.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 91 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 91 kDa
Species Reactivity Database
Immunogen

Recombinant protein of human Cullin 5

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104044

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Cullin 5 Antibody (YA2585)
    Western blot analysis of extracts from C6 (lane 2, 20ug), NIH/3T3 (lane 3, 20ug), Hela (lane 4, 20ug) and PC-3 (lane 5, 20ug) using Cullin 5 Antibody (HY-P82840). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Cullin 5 core component of multiple cullin-5-RING E3 ubiquitin-protein ligase complexes (ECS complexes, also named CRL5 complexes), which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Acts a scaffold protein that contributes to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable SOCS box-containing substrate recognition component. Acts as a key regulator of neuron positioning during cortex development: component of various SOCS-containing ECS complexes, such as the ECS(SOCS7) complex, that regulate reelin signaling by mediating ubiquitination and degradation of DAB1. ECS(SOCS1) seems to direct ubiquitination of JAK2. The ECS(SOCS2) complex mediates the ubiquitination and subsequent proteasomal degradation of phosphorylated EPOR and GHR. The ECS(SPSB3) complex catalyzes ubiquitination of nuclear CGAS. ECS(KLHDC1) complex is part of the DesCEND (destruction via C-end degrons) pathway and mediates ubiquitination and degradation of truncated SELENOS selenoprotein produced by failed UGA/Sec decoding, which ends with a glycine. The ECS(ASB9) complex mediates ubiquitination and degradation of CKB. As part of some ECS complex, promotes 'Lys-11'-linked ubiquitination and degradation of BTRC. As part of a multisubunit ECS complex, polyubiquitinates monoubiquitinated POLR2A. As part of the ECS(RAB40C) complex, mediates ANKRD28 ubiquitination and degradation, thereby inhibiting protein phosphatase 6 (PP6) complex activity and focal adhesion assembly during cell migration. As part of the ECS(RAB40A) complex, mediates RHOU 'Lys-48'-linked ubiquitination and degradation, thus inhibiting focal adhesion disassembly during cell migration. As part of the ECS(RAB40B) complex, mediates LIMA1/EPLIN and RAP2 ubiquitination, thereby regulating actin cytoskeleton dynamics and stress fiber formation during cell migration. May form a cell surface vasopressin receptor; (Microbial infection) Following infection by HIV-1 virus, CUL5 associates with HIV-1 Vif proteins and forms a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex) that catalyzes ubiquitination and degradation of APOBEC3F and APOBEC3G. The complex can also ubiquitinate APOBEC3H to some extent; (Microbial infection) Seems to be involved in proteasomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19].

  • Subcellular Localization

    Nucleus

  • Subunit

    Component of multiple cullin-5-RING E3 ubiquitin-protein ligase complexes (ECS complexes, also named CRL5 complexes) formed of CUL5, Elongin BC (ELOB and ELOC), RNF7/RBX2 and a variable SOCS box domain-containing protein as substrate-specific recognition component (PubMed:11384984, PubMed:15601820, PubMed:16325183, PubMed:21119685, PubMed:27910872, PubMed:32513959, PubMed:33268465, PubMed:35512830, PubMed:26598620, PubMed:33999101, PubMed:35293963). CUL5-containing ECS complexes specifically contain RNF7/RBX2, and not RBX1, as catalytic subunit (PubMed:11384984, PubMed:15601820, PubMed:32513959, PubMed:33268465). Component of the ECS(ASB2) complex with the substrate recognition component ASB2 (PubMed:16325183, PubMed:21119685). Component of the ECS(ASB6) complex with the substrate recognition component ASB6 (PubMed:16325183). Component of the ECS(ASB7) complex with the substrate recognition component ASB7 (PubMed:16325183). Component of the ECS(ASB9) complex with the substrate recognition component ASB9 (PubMed:33268465). Component of the ECS(ASB11) complex with the substrate recognition component ASB11 (PubMed:24337577, PubMed:31387940, PubMed:38574733). Component of the ECS(ASB12) complex with the substrate recognition component ASB12 (PubMed:16325183). Component of the ECS(LRRC41) complex with the substrate recognition component LRRC41 (PubMed:11384984). Component of the ECS(SOCS1) complex with the substrate recognition component SOCS1 (PubMed:11384984). Component of the ECS(SOCS2) complex with the substrate recognition component SOCS2 (PubMed:21980433, PubMed:23897481, PubMed:25505247). Component of the ECS(WSB1) complex with the substrate recognition subunit WSB1 (PubMed:15601820). Component of the ECS(SOCS3) complex with the substrate recognition component SOCS3 (PubMed:15601820). Component of the ECS(SOCS7) complex with the substrate recognition component SOCS7 (By similarity). Component of the ECS(SPSB1) complex with the substrate recognition component SPSB1 (PubMed:15601820). Component of the ECS(SPSB3) complex with the substrate recognition component SPSB3 (PubMed:38418882). Component of the ECS(SPSB2) complex with the substrate recognition component SPSB2 (PubMed:15601820). Component of the ECS(SPSB4) complex with the substrate recognition component SPSB4 (PubMed:15601820). Component of the ECS(RAB40) complex with the substrate recognition subunit RAB40A, RAB40B or RAB40C (PubMed:15601820, PubMed:35512830, PubMed:26598620, PubMed:33999101, PubMed:35293963). Component of the ECS(KLHDC1) complex with the substrate recognition component KLHDC1 (PubMed:32200094). Component of the ECS(PCMTD1) complex with the substrate recognition subunit PCMTD1 (PubMed:35486881). May also form complexes containing RBX1 and ELOA or VHL; additional evidence is however required to confirm this result in vivo (PubMed:10230407, PubMed:11384984, PubMed:18805092, PubMed:19920177). Interacts (when neddylated) with ARIH2; leading to activate the E3 ligase activity of ARIH2 (PubMed:24076655, PubMed:33268465, PubMed:34518685). Interacts with ERCC6; the interaction is induced by DNA damaging agents or inhibitors of RNA polymerase II elongation (PubMed:28292928). Interacts with ELOA (via the BC-box) (PubMed:28292928). Interacts (unneddylated form) with DCUN1D1, DCUN1D2, DCUN1D3, DCUN1D4 and DCUN1D5; these interactions promote the cullin neddylation (PubMed:23201271, PubMed:26906416)

  • SwissProt ID

    Q93034

  • Gene ID
    8065 [NCBI]
  • Synonyms

    CUL-5; VACM1; VACM-1

  • Research Field

    Cell Biology

Cullin 5 Antibody (YA2585) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from C6 (lane 2, 20ug), NIH/3T3 (lane 3, 20ug), Hela (lane 4, 20ug) and PC-3 (lane 5, 20ug) using Cullin 5 Antibody (HY-P82840). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Cullin 5 Antibody (YA2585) Product Image
    1/16
  • Experimental Validation Results for Cullin 5 Antibody (YA2585)
  • Cullin 5 Antibody (YA2585) Product Image
  • Experimental Validation Results for Cullin 5 Antibody (YA2585)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from C6 (lane 2, 20ug), NIH/3T3 (lane 3, 20ug), Hela (lane 4, 20ug) and PC-3 (lane 5, 20ug) using Cullin 5 Antibody (HY-P82840). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Cullin 5 Antibody (YA2585)
  • Cullin 5 Antibody (YA2585) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from C6 (lane 2, 20ug), NIH/3T3 (lane 3, 20ug), Hela (lane 4, 20ug) and PC-3 (lane 5, 20ug) using Cullin 5 Antibody (HY-P82840). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Cullin 5 Antibody (YA2585) Product Image WB