NDUFB10 Antibody (YA1330)(PBS only)

Cat. No.: HY-P81585A: Data Sheet User Guide for Antibodies
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NDUFB10 Antibody (YA1330) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NDUFB10.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

NDUFB10 Antibody (YA1330) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NDUFB10.

Host

Rabbit

Molecular Weight

Predicted band size: 21 kDa;

Observed band size: 21 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O96000

Gene ID

4716 [NCBI]

Immunogen

Recombinant protein of human NDUFB10

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:5000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-1:200
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-1:200
FC FC: Flow Cytometry	1:200-1:1000
IP IP: Immunoprecipitation	1:20-1:50

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Accessory subunit that is involved in the functional assembly of the mitochondrial respiratory chain complex I. Complex I has an NADH dehydrogenase activity with ubiquinone as an immediate electron acceptor and mediates the transfer of electrons from NADH to the respiratory chain

Subcellular Localization：Mitochondrion inner membrane; Peripheral membrane protein; Matrix side

Isoforms & Post-Translational Modification：O96000 has 2 isomers: O96000-1: 20777 Da (predicted); O96000-2: 20143 Da (predicted).
The formation of intramolecular disulfide bonds is assisted by CHCHD4 and ensures folding, import into the mitochondrion and is required for the function in mitochondrial respiratory chain complex I assembly

Subunit：Complex I is composed of 45 different subunits (PubMed:12611891, PubMed:27626371, PubMed:28040730). Interacts with CHCHD4; assists NDUFB10 oxidation, folding and import into mitochondrion (PubMed:28040730)

Research Field

Signal Transduction

Synonyms

NDUFB10; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10; Complex I-PDSW; CI-PDSW; NADH-ubiquinone oxidoreductase PDSW subunit

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/hela-cell.html [Content Brief]