2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. Thymidine Kinase Antibody (YA2195)

Thymidine Kinase Antibody (YA2195)

Cat. No.: HY-P82450
COA
SDS
User Guide for Antibodies Technical Support

Thymidine Kinase Antibody (YA2195) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Thymidine Kinase.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

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제품 설명

Thymidine Kinase Antibody (YA2195) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Thymidine Kinase.
Host

Rabbit
Clonality

Recombinant, Monoclonal
분자량
Predicted band size: 25 kDa;
Observed band size: 25 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

A synthetic peptide of human Thymidine Kinase 1
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IP
IP: Immunoprecipitation
1:20
Sensitivity Endogenous Purity Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
선적

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis of extracts from K562(lane 2(20ug) , Hela(lane 3(20ug) and HEK293(lane 4(20ug) using Thymidine Kinase Antibody (HY-P82450) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background
Function：Cell-cycle-regulated enzyme of importance in nucleotide metabolism (PubMed:9575153). Catalyzes the first enzymatic step in the salvage pathway converting thymidine into thymidine monophosphate (PubMed:22385435). Transcriptional regulation limits expression to the S phase of the cell cycle and transient expression coincides with the oscillation in the intracellular dTTP concentration (Probable). Also important for the activation of anticancer and antiviral nucleoside analog prodrugs such as 1-b-d-arabinofuranosylcytosine (AraC) and 3c-azido-3c-deoxythymidine (AZT) (PubMed:22385435)
Subcellular Localization：Cytoplasm
Subunit：Homotetramer (PubMed:14697231, PubMed:15611477, PubMed:15733844, PubMed:17407781, PubMed:22385435). Tetramerization from dimerization is induced by ATP and increases catalytic efficiency due to a high affinity for thymidine (PubMed:14697231). Tetramerization is inhibited by phosphorylation at Ser-13 (PubMed:14697231). Interacts (via the KEN box) with FZR1 (PubMed:14701726)
RRID
Database

Entrez Gene: 7083 Human

SwissProt: P04183 Human

OMIM: 188300 Human

Research Field

Epigenetics and Nuclear Signaling
Synonyms
EC 2.7.1.21; KITH; TK-1; TK1; Thymidine kinase 1; cytosolic
각종 서류
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Thymidine Kinase Antibody (YA2195) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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상품명:
Thymidine Kinase Antibody (YA2195)
Cat. No.:
HY-P82450
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