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Thymidine Kinase Antibody (YA2195)

(Synonyms: EC 2.7.1.21; KITH; TK-1; TK1; Thymidine kinase 1; cytosolic)
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Based on 1 Customer Validation

Thymidine Kinase Antibody (YA2195) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Thymidine Kinase.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Thymidine Kinase Antibody (YA2195)
  • Thymidine Kinase Antibody (YA2195) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

Thymidine Kinase Antibody (YA2195) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Thymidine Kinase.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 25 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 25 kDa
Species Reactivity Database

Entrez Gene: 7083 Human

SwissProt: P04183 Human

Immunogen

A synthetic peptide of human Thymidine Kinase 1

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104558

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Thymidine Kinase Antibody (YA2195)
    Western blot analysis of extracts from K562(lane 2(20ug) , Hela(lane 3(20ug) and HEK293(lane 4(20ug) using Thymidine Kinase Antibody (HY-P82450) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Thymidine Kinase is a Cell-cycle-regulated enzyme of importance in nucleotide metabolism. Catalyzes the first enzymatic step in the salvage pathway converting thymidine into thymidine monophosphate. Transcriptional regulation limits expression to the S phase of the cell cycle and transient expression coincides with the oscillation in the intracellular dTTP concentration (Probable). Also important for the activation of anticancer and antiviral nucleoside analog prodrugs such as 1-b-d-arabinofuranosylcytosine (AraC) and 3c-azido-3c-deoxythymidine (AZT)[1][2].

  • Subcellular Localization

    Cytoplasm

  • Subunit

    Homotetramer (PubMed:14697231, PubMed:15611477, PubMed:15733844, PubMed:17407781, PubMed:22385435). Tetramerization from dimerization is induced by ATP and increases catalytic efficiency due to a high affinity for thymidine (PubMed:14697231). Tetramerization is inhibited by phosphorylation at Ser-13 (PubMed:14697231). Interacts (via the KEN box) with FZR1 (PubMed:14701726)

  • SwissProt ID

    P04183

  • Gene ID
    7083 [NCBI]
  • Synonyms

    EC 2.7.1.21; KITH; TK-1; TK1; Thymidine kinase 1; cytosolic

  • Research Field

    Epigenetics and Nuclear Signaling

References

[1]. Chang ZF, et al. Serine 13 is the site of mitotic phosphorylation of human thymidine kinase. J Biol Chem. 1998 May 15;273(20):12095-100. [Content Brief]

[2]. Skovgaard T, et al. Comparative active-site mutation study of human and Caenorhabditis elegans thymidine kinase 1. FEBS J. 2012 May;279(10):1777-87. [Content Brief]

Thymidine Kinase Antibody (YA2195) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562(lane 2(20ug) , Hela(lane 3(20ug) and HEK293(lane 4(20ug) using Thymidine Kinase Antibody (HY-P82450) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Thymidine Kinase Antibody (YA2195) Product Image
    1/16
  • Experimental Validation Results for Thymidine Kinase Antibody (YA2195)
  • Thymidine Kinase Antibody (YA2195) Product Image
  • Experimental Validation Results for Thymidine Kinase Antibody (YA2195)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562(lane 2(20ug) , Hela(lane 3(20ug) and HEK293(lane 4(20ug) using Thymidine Kinase Antibody (HY-P82450) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Thymidine Kinase Antibody (YA2195)
  • Thymidine Kinase Antibody (YA2195) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562(lane 2(20ug) , Hela(lane 3(20ug) and HEK293(lane 4(20ug) using Thymidine Kinase Antibody (HY-P82450) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Thymidine Kinase Antibody (YA2195) Product Image WB