Profilin 1 Antibody (YA1796)(PBS only)

Cat. No.: HY-P82051A: Data Sheet User Guide for Antibodies
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Profilin 1 Antibody (YA1796) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Profilin 1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Profilin 1 Antibody (YA1796) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Profilin 1.

Host

Rabbit

Molecular Weight

Predicted band size: 15 kDa;

Observed band size: 15 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P07737

Gene ID

5216 [NCBI]

Immunogen

A synthetic peptide of human Profilin 1

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IP IP: Immunoprecipitation	1:20

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR

Subcellular Localization：Cytoplasm, cytoskeleton

Expression：
Tissue_specificity:Expressed in epididymis (at protein level)

Subunit：Found in a complex with XPO6, Ran, ACTB and PFN1 (PubMed:14592989). Interacts with ACTB (PubMed:10411937). Interacts with VASP (PubMed:17914456, PubMed:18689676). Interacts with HTT (PubMed:18573880). Interacts with SH3BGRL (PubMed:34331014). Occurs in many kinds of cells as a complex with monomeric actin in a 1:1 ratio (PubMed:17914456, PubMed:18689676). Interacts with ACTMAP (PubMed:36173861)

Research Field

Tags & Cell Markers

Synonyms

Epididymis tissue protein Li 184a; Profilin I

Documentation

User Guide for Antibodies (1077 KB)