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EGR1 Antibody (YA467)

(Synonyms: KROX24, ZNF225, EGR1, Early growth response protein 1, EGR-1, AT225, Nerve growth factor-induced protein A, Transcription factor ETR103, Transcription factor Zif268, Zinc finger protein 225, Zinc finger protein Krox-24, NGFI-A)
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Based on 1 Customer Validation

EGR1 Antibody (YA467) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to EGR1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat, Rabbit, Pig

  • Formulation:

    Supplied in PBS (pH7.4),0.1% BSA,40% Glycerol, 0.2% Proclin950.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for EGR1 Antibody (YA467)
  • EGR1 Antibody (YA467) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:1000 1:200-1:1000

Product Details

Description

EGR1 Antibody (YA467) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to EGR1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat, Rabbit, Pig
  • Observed Molecular Weight
    Observed band size: 75 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 58 kDa
Species Reactivity Database

Entrez Gene: 1958 Human

SwissProt: P18146 Human

Immunogen

Synthetic peptide corresponding to Human EGR1.AA range:1-200.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102309

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4),0.1% BSA,40% Glycerol, 0.2% Proclin950.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for EGR1 Antibody (YA467)
    Western blot analysis of extracts from 293T (lane 1) and THP-1 (lane 2) and Jurkat (lane 3) using EGR1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

Background

  • Function

    EGR1 transcriptional regulator. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'(EGR-site) in the promoter region of target genes. Binds double-stranded target DNA, irrespective of the cytosine methylation status. Regulates the transcription of numerous target genes, and thereby plays an important role in regulating the response to growth factors, DNA damage, and ischemia. Plays a role in the regulation of cell survival, proliferation and cell death. Activates expression of p53/TP53 and TGFB1, and thereby helps prevent tumor formation. Required for normal progress through mitosis and normal proliferation of hepatocytes after partial hepatectomy. Mediates responses to ischemia and hypoxia; regulates the expression of proteins such as IL1B and CXCL2 that are involved in inflammatory processes and development of tissue damage after ischemia. Regulates biosynthesis of luteinizing hormone (LHB) in the pituitary. Regulates the amplitude of the expression rhythms of clock genes: BMAL1, PER2 and NR1D1 in the liver via the activation of PER1 (clock repressor) transcription. Regulates the rhythmic expression of core-clock gene BMAL1 in the suprachiasmatic nucleus (SCN)[1][2][3].

  • Subcellular Localization

    Nucleus; Cytoplasm

  • Expression


    Tissue_specificity:Protein levels were detected in neutrophils.

    Induction:By growth factors

  • Subunit

    Interacts with SNAI1 and SP1 upon 12-O-tetradecanoylphorbol-13-acetate (TPA) induction

  • SwissProt ID

    P18146

  • Gene ID
    1958 [NCBI]
  • Synonyms

    KROX24, ZNF225, EGR1, Early growth response protein 1, EGR-1, AT225, Nerve growth factor-induced protein A, Transcription factor ETR103, Transcription factor Zif268, Zinc finger protein 225, Zinc finger protein Krox-24, NGFI-A

  • Research Field

    Signal Transduction

[1]. Hu CT, et al. Snail associates with EGR-1 and SP-1 to upregulate transcriptional activation of p15INK4b. FEBS J. 2010 Mar;277(5):1202-18. [Content Brief]

[2]. Hashimoto H, et al. Wilms tumor protein recognizes 5-carboxylcytosine within a specific DNA sequence. Genes Dev. 2014 Oct 15;28(20):2304-13. [Content Brief]

[3]. Zandarashvili L, et al. Structural impact of complete CpG methylation within target DNA on specific complex formation of the inducible transcription factor Egr-1. FEBS Lett. 2015 Jul 8;589(15):1748-53. [Content Brief]

EGR1 Antibody (YA467) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from 293T (lane 1) and THP-1 (lane 2) and Jurkat (lane 3) using EGR1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • EGR1 Antibody (YA467) Product Image
    1/16
  • Experimental Validation Results for EGR1 Antibody (YA467)
  • EGR1 Antibody (YA467) Product Image
  • Experimental Validation Results for EGR1 Antibody (YA467)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from 293T (lane 1) and THP-1 (lane 2) and Jurkat (lane 3) using EGR1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for EGR1 Antibody (YA467)
  • EGR1 Antibody (YA467) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from 293T (lane 1) and THP-1 (lane 2) and Jurkat (lane 3) using EGR1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • EGR1 Antibody (YA467) Product Image WB