Elongation factor 1 gamma Antibody (YA2004)

Cat. No.: HY-P82259: Data Sheet COA
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Elongation factor 1 gamma Antibody (YA2004) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Elongation factor 1 gamma.

For research use only. We do not sell to patients.

Size	Stock	Quantity
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10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Elongation factor 1 gamma Antibody (YA2004) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation
References

Description

Elongation factor 1 gamma Antibody (YA2004) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Elongation factor 1 gamma.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 50 kDa;

Observed band size: 50 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P26641

Gene ID

1937 [NCBI]

Immunogen

A synthesized peptide derived from human EEF1G aa52-98/437.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-1:200
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IP IP: Immunoprecipitation	1:50
FC FC: Flow Cytometry	1:50-1:100

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Liquid in 50 mM Tris-Glycine(pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

ALL WB ICC

Western blot analysis of extracts from HEK293T(lane 2(20ug) , HepG2(lane 3(20ug) and NIH/3T3(lane 4(20ug),MCF-7(lane 5(20ug) using Elongation factor 1 gamma Antibody (HY-P82259) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Immunocytochemistry analysis of MCF-7 cells labeling Elongation factor 1 gamma with Elongation factor 1 gamma Antibody (HY-P82259) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Elongation factor 1 gamma Antibody (HY-P82259) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of PANC-1 cells labeling Elongation factor 1 gamma with Elongation factor 1 gamma Antibody (HY-P82259) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Elongation factor 1 gamma Antibody (HY-P82259) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：Probably plays a role in anchoring the complex to other cellular components

Expression：
Tissue_specificity:Highly expressed in pancreatic tumor tissue and to a lesser extent in normal kidney, intestine, pancreas, stomach, lung, brain, spleen and liver

Induction:Down-regulated in response to enterovirus 71 (EV71) infection

Isoforms & Post-Translational Modification：P26641 has 2 isomers: P26641-1: 50119 Da (predicted); P26641-2: 56150 Da (predicted).

Subunit：EF-1 is composed of four subunits: alpha, beta, delta, and gamma

Database

Entrez Gene: 1937 Human ; 67160 Mouse ; 293725 Rat

SwissProt: P26641 Human ; Q9D8N0 Mouse ; Q68FR6 Rat

OMIM: 130593 Human

Research Field

Epigenetics and Nuclear Signaling

Synonyms

EEF1G; EF1G; Elongation factor 1-gamma; EF-1-gamma; eEF-1B gamma

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/hct-116-cell.html [Content Brief]

Elongation factor 1 gamma Antibody (YA2004) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Elongation factor 1 gamma Antibody (YA2004)

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Background

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