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Elongation factor 1 gamma Antibody (YA2004)

(Synonyms: EEF1G; EF1G; Elongation factor 1-gamma; EF-1-gamma; eEF-1B gamma)
Customer Review

Based on 1 Customer Validation

Elongation factor 1 gamma Antibody (YA2004) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Elongation factor 1 gamma.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Liquid in 50 mM Tris-Glycine(pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for Elongation factor 1 gamma Antibody (YA2004)
  • Experimental Validation Results for Elongation factor 1 gamma Antibody (YA2004)
  • Experimental Validation Results for Elongation factor 1 gamma Antibody (YA2004)
  • Elongation factor 1 gamma Antibody (YA2004) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000-1:2000 1:100-1:200 1:50-1:200 1:50 1:50-1:100

Product Details

Description

Elongation factor 1 gamma Antibody (YA2004) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Elongation factor 1 gamma.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 50 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 50 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human EEF1G aa52-98/437.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Liquid in 50 mM Tris-Glycine(pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Elongation factor 1 gamma Antibody (YA2004)
    Western blot analysis of extracts from HEK293T(lane 2(20ug) , HepG2(lane 3(20ug) and NIH/3T3(lane 4(20ug),MCF-7(lane 5(20ug) using Elongation factor 1 gamma Antibody (HY-P82259) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Elongation factor 1 gamma Antibody (YA2004)
    Immunocytochemistry analysis of MCF-7 cells labeling Elongation factor 1 gamma with Elongation factor 1 gamma Antibody (HY-P82259) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Elongation factor 1 gamma Antibody (HY-P82259) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Elongation factor 1 gamma Antibody (YA2004)
    Immunocytochemistry analysis of PANC-1 cells labeling Elongation factor 1 gamma with Elongation factor 1 gamma Antibody (HY-P82259) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Elongation factor 1 gamma Antibody (HY-P82259) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Elongation factor 1 gamma is a Probably plays a role in anchoring the complex to other cellular components

  • Expression


    Tissue_specificity:Highly expressed in pancreatic tumor tissue and to a lesser extent in normal kidney, intestine, pancreas, stomach, lung, brain, spleen and liver

    Induction:Down-regulated in response to enterovirus 71 (EV71) infection

  • Isoforms & Post-Translational Modification

    P26641 has 2 isomers: P26641-1: 50119 Da (predicted); P26641-2: 56150 Da (predicted).

  • Subunit

    EF-1 is composed of four subunits: alpha, beta, delta, and gamma

  • SwissProt ID

    P26641

  • Gene ID
    1937 [NCBI]
  • Synonyms

    EEF1G; EF1G; Elongation factor 1-gamma; EF-1-gamma; eEF-1B gamma

  • Research Field

    Epigenetics and Nuclear Signaling

Elongation factor 1 gamma Antibody (YA2004) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293T(lane 2(20ug) , HepG2(lane 3(20ug) and NIH/3T3(lane 4(20ug),MCF-7(lane 5(20ug) using Elongation factor 1 gamma Antibody (HY-P82259) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling Elongation factor 1 gamma with Elongation factor 1 gamma Antibody (HY-P82259) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Elongation factor 1 gamma Antibody (HY-P82259) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of PANC-1 cells labeling Elongation factor 1 gamma with Elongation factor 1 gamma Antibody (HY-P82259) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Elongation factor 1 gamma Antibody (HY-P82259) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Elongation factor 1 gamma Antibody (YA2004) Product Image
    1/16
  • Experimental Validation Results for Elongation factor 1 gamma Antibody (YA2004)
  • Experimental Validation Results for Elongation factor 1 gamma Antibody (YA2004)
  • Experimental Validation Results for Elongation factor 1 gamma Antibody (YA2004)
  • Elongation factor 1 gamma Antibody (YA2004) Product Image
  • Experimental Validation Results for Elongation factor 1 gamma Antibody (YA2004)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293T(lane 2(20ug) , HepG2(lane 3(20ug) and NIH/3T3(lane 4(20ug),MCF-7(lane 5(20ug) using Elongation factor 1 gamma Antibody (HY-P82259) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Elongation factor 1 gamma Antibody (YA2004)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling Elongation factor 1 gamma with Elongation factor 1 gamma Antibody (HY-P82259) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Elongation factor 1 gamma Antibody (HY-P82259) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Elongation factor 1 gamma Antibody (YA2004)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of PANC-1 cells labeling Elongation factor 1 gamma with Elongation factor 1 gamma Antibody (HY-P82259) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Elongation factor 1 gamma Antibody (HY-P82259) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Elongation factor 1 gamma Antibody (YA2004)
  • Experimental Validation Results for Elongation factor 1 gamma Antibody (YA2004)
  • Experimental Validation Results for Elongation factor 1 gamma Antibody (YA2004)
  • Elongation factor 1 gamma Antibody (YA2004) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293T(lane 2(20ug) , HepG2(lane 3(20ug) and NIH/3T3(lane 4(20ug),MCF-7(lane 5(20ug) using Elongation factor 1 gamma Antibody (HY-P82259) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Elongation factor 1 gamma Antibody (YA2004) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling Elongation factor 1 gamma with Elongation factor 1 gamma Antibody (HY-P82259) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Elongation factor 1 gamma Antibody (HY-P82259) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Elongation factor 1 gamma Antibody (YA2004) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of PANC-1 cells labeling Elongation factor 1 gamma with Elongation factor 1 gamma Antibody (HY-P82259) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Elongation factor 1 gamma Antibody (HY-P82259) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Elongation factor 1 gamma Antibody (YA2004) Product Image WB
  • Elongation factor 1 gamma Antibody (YA2004) Product Image ICC
  • Elongation factor 1 gamma Antibody (YA2004) Product Image ICC