EPCAM Antibody (YA3827)(PBS only)

Cat. No.: HY-P84130A: Ficha de datos User Guide for Antibodies
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EPCAM Antibody (YA3827) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to EPCAM.

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Tamaño		Stock
50 μL		Obtener un presupuesto
100 μL		Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Descripciòn

Descripciòn

EPCAM Antibody (YA3827) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to EPCAM.

Host

Mouse

Peso molecular

Predicted band size: 35 kDa;

Observed band size: 35 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P16422

Gene ID

4072 [NCBI]

Immunogen

Purified recombinant fragment of human EPCAM (AA: 24-265) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Pureza	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Background

Function：May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E

Subcellular Localization：Lateral cell membrane; Single-pass type I membrane protein; Cell junction, tight junction

Expression：
Tissue_specificity:This gene is highly and selectively expressed in undifferentiated embryonic stem cells (ESCs) , but not in differentiated ESCs. Once ESCs differentiate, its protein level rapidly declines. This gene is expressed on almost all epithelial cell membranes, but not on mesoderm or neural cell membranes. It has also been found on the surface of adenocarcinomas.

Subunit：Monomer. Interacts with phosphorylated CLDN7

Synonyms

ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1

Documentación

User Guide for Antibodies (1077 KB)