Copy Link Print Information

XIAP Antibody (YA5906)

(Synonyms: XIAP; API3; BIRC4; IAP3; E3 ubiquitin-protein ligase XIAP; Baculoviral IAP repeat-containing protein 4; IAP-like protein; ILP; hILP; Inhibitor of apoptosis protein 3; IAP-3; hIAP-3; hIAP3; X-linked inhibitor of apoptosis protein; X-linked I)
Customer Review

Based on 1 Customer Validation

XIAP Antibody (YA5906) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to XIAP.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for XIAP Antibody (YA5906)
  • Experimental Validation Results for XIAP Antibody (YA5906)
  • XIAP Antibody (YA5906) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:2000-1:10000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

XIAP Antibody (YA5906) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to XIAP.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 57 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 57 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for XIAP Antibody (YA5906)
    Western blot analysis of extracts from Hela (lane 2, 20ug), C6 (lane3, 20μg) and Jurkat (lane4, 20μg) using XIAP Antibody (HY-P86214). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for XIAP Antibody (YA5906)
    Western blot analysis of extracts from A549 (lane 1(20μg)) and C6 (lane 2(20μg)) using XIAP Antibody (HY-P86214) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    XIAP is a core inhibitor of apoptosis protein that directly restrains executioner caspase-3 and caspase-7 through BIR2-region mechanisms and inhibits initiator caspase-9 through BIR3 binding[1][2][3]. Mechanistically, its RING domain also supports E3 ubiquitin-ligase activity, linking XIAP biology to protein stability, apoptosis regulation, and non-apoptotic signaling contexts[4]. In innate immune signaling, XIAP ubiquitylates RIPK2 and recruits LUBAC after NOD2 stimulation, thereby enabling NF-κB activation and proinflammatory cytokine secretion[5]. In disease models and patient studies, XIAP deficiency associates with Crohn disease-like inflammatory bowel disease and defective NOD2 function in monocytes[6]. Compared with related cIAP1 and cIAP2 isoforms, XIAP has the clearest biochemical role as a direct caspase inhibitor, whereas cIAP1 and cIAP2 bind but do not efficiently inhibit caspases[7]. For experimental applications, selective XIAP antagonism can block NOD2-mediated inflammatory signaling by disrupting the XIAP-RIPK2 interaction, while broader IAP antagonists are used to test apoptosis sensitization mechanisms involving XIAP and cIAP proteins[8][9].- XIAP directly controls caspase-dependent apoptosis and connects apoptosis biology with inflammatory NOD2 signaling[1][5].- XIAP differs from cIAP1/cIAP2 because it directly inhibits caspase activity[7].- XIAP-RIPK2 antagonism provides a practical model for studying NOD2-driven inflammation[8].

  • Subcellular Localization

    Cytoplasm; Nucleus

  • Expression


    Tissue_specificity:Expressed in colonic crypts (at protein level) (PubMed:30389919) . Ubiquitous, except peripheral blood leukocytes (PubMed:8654366)

  • Subunit

    Monomer, and homodimer. Part of a complex composed of SEPTIN4 isoform ARTS, XIAP and BCL2, within the complex interacts with SEPTIN4 isoform ARTS and BCL2, SEPTIN4 isoform ARTS acts as a scaffold protein and stabilizes the complex (PubMed:29020630). Interacts (via BIR3 domain) with DIABLO/SMAC; the interaction inhibits apoptotic suppressor activity (PubMed:11140637, PubMed:11257230, PubMed:21695558). Interacts with HTRA2/PRSS25; the interaction inhibits apoptotic suppressor activity (PubMed:11604410). Interacts with TAB1/MAP3K7IP1 and AIFM1. Interaction with DIABLO/SMAC hinders binding of TAB1/MAP3K7IP1 and AIFM1. Interacts with TCF25 and COMMD1. Interacts (via BIR3 domain) with SEPTIN4 isoform ARTS (PubMed:15029247, PubMed:21695558). Interacts (via BIR3 domain) with SEPTIN4 (By similarity). Interacts with RIP1, RIP2, RIP3, RIP4, CCS and USP19. Interacts (via BIR 2 domain and BIR 3 domain) with HAX1 (via C-terminus) and this interaction blocks ubiquitination of XIAP/BIRC4. Interacts with the monomeric form of BIRC5/survivin. Interacts with TLE3 and TCF7L2/TCF4. Interacts (via BIR 3 and RING domains) with PDCL3 (PubMed:19012568)

  • SwissProt ID

    P98170

  • Gene ID
    331 [NCBI]
  • Synonyms

    XIAP; API3; BIRC4; IAP3; E3 ubiquitin-protein ligase XIAP; Baculoviral IAP repeat-containing protein 4; IAP-like protein; ILP; hILP; Inhibitor of apoptosis protein 3; IAP-3; hIAP-3; hIAP3; X-linked inhibitor of apoptosis protein; X-linked I

References

[1]. Riedl SJ, et al. Structural basis for the inhibition of caspase-3 by XIAP. Cell. 2001 Mar 9;104(5):791-800. [Content Brief]

[2]. Chai J, et al. Structural basis of caspase-7 inhibition by XIAP. Cell. 2001 Mar 9;104(5):769-80. [Content Brief]

[3]. Shiozaki EN, et al. Mechanism of XIAP-mediated inhibition of caspase-9. Mol Cell. 2003 Feb;11(2):519-27. [Content Brief]

[4]. Galbán S, et al. XIAP as a ubiquitin ligase in cellular signaling. Cell Death Differ. 2010 Jan;17(1):54-60. [Content Brief]

[5]. Damgaard RB, et al. The ubiquitin ligase XIAP recruits LUBAC for NOD2 signaling in inflammation and innate immunity. Mol Cell. 2012 Jun 29;46(6):746-58. [Content Brief]

[6]. Aguilar C, et al. Characterization of Crohn disease in X-linked inhibitor of apoptosis-deficient male patients and female symptomatic carriers. J Allergy Clin Immunol. 2014 Nov;134(5):1131-41.e9. [Content Brief]

[7]. Eckelman BP, et al. The human anti-apoptotic proteins cIAP1 and cIAP2 bind but do not inhibit caspases. J Biol Chem. 2006 Feb 10;281(6):3254-60. [Content Brief]

[8]. Goncharov T, et al. Disruption of XIAP-RIP2 Association Blocks NOD2-Mediated Inflammatory Signaling. Mol Cell. 2018 Feb 15;69(4):551-565.e7. [Content Brief]

[9]. Ndubaku C, et al. Antagonism of c-IAP and XIAP proteins is required for efficient induction of cell death by small-molecule IAP antagonists. ACS Chem Biol. 2009 Jul 17;4(7):557-66. [Content Brief]

View More

XIAP Antibody (YA5906) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2, 20ug), C6 (lane3, 20μg) and Jurkat (lane4, 20μg) using XIAP Antibody (HY-P86214). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A549 (lane 1(20μg)) and C6 (lane 2(20μg)) using XIAP Antibody (HY-P86214) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • XIAP Antibody (YA5906) Product Image
    1/16
  • Experimental Validation Results for XIAP Antibody (YA5906)
  • Experimental Validation Results for XIAP Antibody (YA5906)
  • XIAP Antibody (YA5906) Product Image
  • Experimental Validation Results for XIAP Antibody (YA5906)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2, 20ug), C6 (lane3, 20μg) and Jurkat (lane4, 20μg) using XIAP Antibody (HY-P86214). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for XIAP Antibody (YA5906)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A549 (lane 1(20μg)) and C6 (lane 2(20μg)) using XIAP Antibody (HY-P86214) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for XIAP Antibody (YA5906)
  • Experimental Validation Results for XIAP Antibody (YA5906)
  • XIAP Antibody (YA5906) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2, 20ug), C6 (lane3, 20μg) and Jurkat (lane4, 20μg) using XIAP Antibody (HY-P86214). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • XIAP Antibody (YA5906) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A549 (lane 1(20μg)) and C6 (lane 2(20μg)) using XIAP Antibody (HY-P86214) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • XIAP Antibody (YA5906) Product Image WB
  • XIAP Antibody (YA5906) Product Image WB