NCoA-3 Antibody (YA4615)(PBS only)

Cat. No.: HY-P84918A: Ficha de datos User Guide for Antibodies
FAQs
Technical Support

NCoA-3 Antibody (YA4615) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NCoA-3.

Para uso exclusivo en investigación. No vendemos a pacientes.

Tamaño		Stock
50 μL		Obtener un presupuesto
100 μL		Obtener un presupuesto

* Seleccione Cantidad antes de agregar artículos.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Descripciòn
Referencias

Descripciòn

NCoA-3 Antibody (YA4615) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NCoA-3.

Host

Mouse

Peso molecular

Predicted band size: 155 kDa;

Observed band size: 150-160 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9Y6Q9

Gene ID

8202 [NCBI]

Immunogen

Purified recombinant fragment of human AIB1 aa 276-398.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Pureza	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Background

Function：Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit

Subcellular Localization：Cytoplasm; Nucleus

Expression：
Tissue_specificity:Widely expressed. Highly expressed in the heart, skeletal muscle, pancreas, and placenta. Lowly expressed in the brain, and extremely lowly expressed in the lungs, liver, and kidneys.

Isoforms & Post-Translational Modification：Q9Y6Q9 has 5 isomers: Q9Y6Q9-1: 155293 Da (predicted); Q9Y6Q9-2: 153797 Da (predicted); Q9Y6Q9-3: 154400 Da (predicted); Q9Y6Q9-4: 147499 Da (predicted); Q9Y6Q9-5: 154892 Da (predicted).
Acetylated by CREBBP. Acetylation occurs in the RID domain, and disrupts the interaction with nuclear receptors and regulates its function;Methylated by CARM1;Phosphorylated by IKK complex. Regulated its function. Phosphorylation at Ser-601 by CK1 promotes coactivator function

Subunit：Present in a complex containing NCOA2, IKKA, IKKB, IKBKG and the histone acetyltransferase protein CREBBP (PubMed:11971985, PubMed:9346901).

Synonyms

AIB; AIB1; ANIB1

Documentación

User Guide for Antibodies (1077 KB)

Referencias

[1]. /biology-dictionary/rcc10rgb-cell.html